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Rice rhizosphere burkholderia and application thereof in prevention and control of rice sheath blight disease

A technology for rice sheath blight and Burkholderia, which is applied in the application, bacteria, fungicides and other directions to achieve the effects of low protease activity, high safety in use, and good inhibitory effect.

Inactive Publication Date: 2012-08-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the use of Burkholderia cepacia strains to control rice sheath blight has certain potential risks, and it is necessary to find a safer and more efficient Burkholderia strain for the control of rice sheath blight

Method used

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  • Rice rhizosphere burkholderia and application thereof in prevention and control of rice sheath blight disease
  • Rice rhizosphere burkholderia and application thereof in prevention and control of rice sheath blight disease
  • Rice rhizosphere burkholderia and application thereof in prevention and control of rice sheath blight disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Isolation and Purification of Bacterial Strains

[0018] The 10-20cm surface soil around the rice rhizosphere was collected from a paddy field in Hangzhou City, Zhejiang Province, put into a sterile polyethylene plastic bag, and sealed. Weigh 10g of soil, add it into a triangular flask with 90ml sterile water to prepare a suspension, shake it at 180r / min for 30min, then draw 5ml from the resulting suspension and add it to a triangular flask with 45ml sterile water, and gradually dilute to Until the 5th triangular flask, get 10 -1 、10 -2 、10 -3 、10 -4 and 10 -5 diluent. Take 100μl 10 -4 and 10 -5 The dilutions of the above-mentioned nutrient agar medium were spread on the nutrient agar medium, and each dilution was repeated 3 times. After being cultured in a 30°C constant temperature incubator for 48 hours, a single colony with different cultural characteristics was picked and purified as the test strain. . Through the inhibition zone experiment of the...

Embodiment 2

[0020] Example 2 Identification of fatty acids

[0021] The specific test method is as follows:

[0022] 1. Place the strain at 28°C and culture it on trypticase soybean agar medium (containing: tryptone 15g, soybean peptone 5g, sodium chloride 5g, agar 15g, water 1000ml) for 24h;

[0023] 2. Use the MIDI fatty acid identification system to determine the fatty acid composition of the above strains;

[0024] 3. According to the comparison of the determined fatty acid composition of the strain and the MIDI identification database TSBA50 version 5.00, the strain was identified.

[0025] According to the instruction manual of the microbial fatty acid identification system, a similarity value of 0.6 or higher indicates a good match, while a similarity value of 0.4-0.6 indicates that the two are not the same species. Compared with the species in the aerobic bacteria database in the microbial fatty acid identification system, it was found that the similarity value of this strain to...

Embodiment 3

[0026] Example 3 Biolog Carbon Source Utilization Identification

[0027] The biolog method was used to detect the utilization rate of the carbon source by the strain, and the specific method was as follows: the strain was placed in the tryptic casein soybean agar culture solution, cultured at 28°C for 24 hours, and the strain was detected by the Biolog Gram-negative plate The utilization rate of carbon source was compared with the Biolog identification database GN 4.01.

[0028] The results showed that the strain could utilize lactose and melibiose, and the strain was identified as Burkholderia cepacia, with a similarity index of 0.65.

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Abstract

The invention discloses burkholderia for preventing and controlling rice sheath blight disease, which was identified and named burkholderia R456 and collected in China Center for Type Culture Collection (CCTCC) on 20th August, 2010, the CCTCC is located in Wuhan University, Luojia Mountain, Wuhan and the collection number is CCTCC No.M2010207. The burkholderia R456 in the invention has a relatively strong suppression effect on the growth of rhizoctonia solani under in-vitro, greenhouse and field conditions and has good prevention and control effect on rice sheath blight disease. Compared withonion burkholderia, the burkholderia R456 is not harmful to the plants and is very safe to be used as a biocontrol strain for preventing and controlling rice sheath blight disease, and thus, it is proved that the rhizosphere bacteria can be used as the biocontro strain for preventing and controlling rice sheath blight disease.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to Burkholderia oryzae rhizosphere and its application in preventing and treating rice sheath blight. Background technique [0002] Rice is an important food crop, many diseases can lead to serious reduction in its yield. Among them, rice sheath blight caused by Rhizoctonia solani is a devastating disease that commonly occurs in rice-producing areas in the world, and constitutes the four major diseases of rice together with rice blast, bacterial blight and thin strip disease. Rice sheath blight, also known as "cloud wilt", mostly occurs under high temperature and high humidity conditions. According to statistics, the current incidence area of ​​rice sheath blight in China has reached 13.3333 million hm 2 More than 40% of the total rice planting area. The stalks and leaf sheaths of the affected rice plants are dry to rotten, unable to head, or there are more grains in the head, the seed setti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
Inventor 李斌石雨吴志毅刘宝平陶中云谢关林
Owner ZHEJIANG UNIV
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