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Incident differential interference phase contrast microscope

A phase-contrast microscope and differential interference technology, which is applied in the field of epi-type differential interference phase-contrast microscopy, can solve the problems of complex microscope structure, DIC prism design, complex processing and detection, etc., and achieve simple structure, uniform field of view, and convenient operation. Effect

Active Publication Date: 2011-03-30
GUANGZHOU LISS OPTICAL INSTR
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Problems solved by technology

In the existing epi-type differential interference contrast microscope, a differential interference contrast prism device is added to the polarization observation system. The illumination and imaging systems share the same DIC prism. New requirements are put forward for the design of objective lenses with different magnifications. Special objective lenses for differential interference phase contrast observation need to be configured, which makes the structure of the microscope complicated. At the same time, the design, processing and detection of DIC prisms are relatively complicated.

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Embodiment Construction

[0025] like figure 1 As mentioned above, the epi-differential interference contrast microscope of the present invention includes a binocular observation mirror 1, a photographic camera output port 2, an epi-illuminator 3, a converter 4, an epi-differential interference contrast component 5, an objective lens 6, The object platform 7 , the coarse and fine focus adjustment mechanism 8 , the Abbe condenser lens group 9 and the light collector 10 .

[0026] Among them, the binocular observation lens 1 adopts a hinged structure and is equipped with a wide-angle plan eyepiece. The unilateral diopter can be adjusted within the range of -5 to +5 diopter. The eyepiece pupil distance can be adjusted arbitrarily between 53mm and 75mm. The inclination angle of the tube is 30 degrees, and it can be adapted to eyepieces whose field of view does not exceed Ф22mm.

[0027] Photographic camera output port 2 is an optical output terminal used for microscope image acquisition. With a special ph...

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Abstract

The invention belongs to the technical field of microscopes, in particular to an incident differential interference phase contrast microscope. The microscope comprises an observation mirror, an incident illuminator and a differential interference phase contrast component, wherein the differential interference phase contrast component comprises a differential interference prism, an adjusting rod for adjusting the forward and backward movement of the differential interference prism, and an adjusting screw for adjusting the vertical height of the differential interference prism. The incident differential interference phase contrast microscope has the functions of upright observation and polarization observation of a bright field, can perform differential interference phase contrast observation, has a simple structure, is convenient to operate, can acquire micro-images with a uniform field and obvious contrast, can be applied to the fields of modern electronic industry, material scientific research and manufacturing, and the like, and meets the requirement of nondestructive optical detection.

Description

technical field [0001] The invention belongs to the technical field of microscopes, and in particular relates to an epi-type differential interference phase contrast microscope, which enables the observed microscopic images to have obvious contrast contrast and presents images with strong stereoscopic effect, so as to improve the microscopic observation. resolution. Background technique [0002] Differential interference contrast microscopy technology was proposed by Nomarski in 1952 on the basis of phase contrast microscopy observation technology, using a transmission microscopy observation system, including polarizer, DIC prism, DIC slider and analyzer (Analyzer) four key optical components. Science and technology workers such as Xu Yuguang, Shanghai Institute of Optics and Fine Mechanics of the Chinese Academy of Sciences have carried out technical research on differential interference contrast microscopy, and in July 1985, they applied to the State Intellectual Property...

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Application Information

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IPC IPC(8): G02B21/14G02B21/18
Inventor 李弥高黄文勇黄秋翼胡丽娟
Owner GUANGZHOU LISS OPTICAL INSTR
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