Application of formononetin in preparing of medicament for restricting angiogenesis

A technology of formononetin and angiogenesis, which is applied in the direction of drug combination, cardiovascular system diseases, anti-tumor drugs, etc., to achieve the effect of inhibiting proliferation

Inactive Publication Date: 2011-05-11
SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Abstract
  • Description
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  • Application of formononetin in preparing of medicament for restricting angiogenesis
  • Application of formononetin in preparing of medicament for restricting angiogenesis
  • Application of formononetin in preparing of medicament for restricting angiogenesis

Examples

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Example Embodiment

[0032] Example 1 Formononetin inhibits the proliferation of human umbilical vein endothelial cells (HUVEC)

[0033] The CCK-8 method was used to detect the proliferation of HUVEC. CCK-8 reagent contains WST-8(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfonic acid benzene) -2H-tetrazole monosodium salt), which is reduced by dehydrogenase in the mitochondria of the cell under the action of the electron carrier 1-Methoxy PMS (1-methoxy-5-methylphenazine dimethyl sulfate) to have Highly water-soluble yellow formazan (Formazan) product. The amount of formazan produced is directly proportional to the number of living cells. The light absorption value measured at 450 nm with an enzyme-linked immunoassay can indirectly reflect the number of living cells. This method can be used to evaluate the effects of drugs on the proliferation of HUVCE.

[0034] Method: HUVEC cells were seeded and cultured in 96-well plates. After the cells have grown to 80% confluence, add formonon...

Example Embodiment

[0038] Example 2 Formononetin inhibits migration of human umbilical vein endothelial cells (HUVEC)

[0039] Transwell method detects HUVEC migration. Transwell is an experimental technology. The main material of this technology is the Transwell chamber. The bottom layer of the chamber has a permeable membrane (polycarbonate membrane is commonly used, which has pores). Micropores with a size of 0.1-12.0 μm. In the experiment, the cells are placed on the chamber. Under the action of the chemokine or growth factor contained in the chamber, the cells penetrate the polycarbonate membrane for co-culture, cell chemotaxis, and cell Research on migration and cell invasion.

[0040] Method: Add 600 μL of VEGF containing 20 ng / ml to the lower chamber 165 HUVEC culture medium. Add HUVEC containing different concentrations of formononetin into the upper chamber (cell density of 2×10 5 / ml), placed in CO 2 Culture in a cell incubator for 8 hours. The group without formononetin was used as co...

Example Embodiment

[0042] Example 3 Formononetin inhibits HUVEC tubule formation experiment

[0043] Matrigel glue was used to detect the tubule formation of HUVEC. Artificial basement membrane Matrigel glue is a basement membrane component extracted from mouse EHS sarcoma. It can spontaneously form a gel-like artificial basement membrane at 37 ℃, which has the biological function of natural basement membrane. Human umbilical vein endothelial cells can be adhered to Matrigel glue into tubes, which can be used to study the effect of drugs on the formation of endothelial cells.

[0044] Method: Take 50 μl of Matrigel glue solution and add it to a pre-cooled 96-well culture plate, then place it in a 37 ℃ incubator and incubate for 1 h to solidify the glue. The HUVEC containing different concentrations of formononetin (cell density of 1×10 5 / ml) were added to the 96-well plate covered with Matrigel and placed in CO 2 Culture in a cell incubator for 10 h. The group without formononetin was used as co...

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Abstract

The invention relates to application of formononetin in preparing of a medicament for restricting angiogenesis. The invention has the advantages that: the new application of the formononetin is provided, the fact that the formononetin can effectively restrict the propagation and the migration of endothelial cells in veins and canaliculization is found first, and firstly found in the invention, the formononetin has an anti-angiogenesis activity and can be used as an angiogenesis depressor. The invention firstly finds that the formononetin has the anti-angiogenesis action and can be applied to the preparation of the angiogenesis medicament as an angiogenesis depressor so as to treat angiogenesis-dependent and angiogenesis-associated diseases, such as tumors, arthritis, psoriasis, eye diseases, atherosclerosis, and the like.

Description

technical field [0001] The invention relates to the application of a Chinese herbal medicine monomer, in particular, the application of a Chinese herbal medicine monomer formononetin in the preparation of an angiogenesis-inhibiting drug. Background technique [0002] Angiogenesis is the process by which new blood vessels develop from existing capillaries. Angiogenesis is a complex process involving a variety of cells and molecules, mainly including the following processes: vascular basement membrane degradation in the activation phase; activation, proliferation, and migration of vascular endothelial cells; and reconstruction of new blood vessels and vascular networks. [0003] Blood vessels are an important way to transport oxygen, nutrients and metabolites, and it is difficult for tissues or organs without vascular support to survive. [0004] Neovascularization occurs under both physiological and pathological conditions. Physiological angiogenesis mainly occurs during em...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P35/00A61P35/02A61P17/06A61P19/02A61P9/10A61P27/02
Inventor 赵梅陈红专方超陆琴白帆王超於得红
Owner SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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