Method for detecting plasma concentration of ciclosporin A by adopting competition method

A technology of blood drug concentration and detection ring, applied in the field of detection of cyclosporine A, can solve the problems of large individual differences in pharmacokinetic parameters, narrow effective therapeutic concentration range, long medication time, etc., and achieves good application prospects and fast measurement speed. , the effect of less sample consumption

Inactive Publication Date: 2011-06-15
SHANGHAI GENEXT MEDICAL TECH
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Problems solved by technology

Cyclosporine A is incompletely absorbed orally, takes a long time to take medication, has large individual differences in pharmacokinetic parameters, and has a narrow range of effective therapeutic concentrations

Method used

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Embodiment 2

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Abstract

The invention relates to a method for detecting plasma concentration of ciclosporin A by adopting a competition method, which comprises the steps of: coupling the ciclosporin A on the surface of a magnetic bead, adding a plasma sample after anticoagulation and dilution in a sample hole, adding a ciclosporin A monoclonal antibody marked by fluorescein, eluting the mixture, detecting the fluorescence intensity under excitation of exciting light, and obtaining the plasma concentration of ciclosporin A. The method for detecting plasma concentration of ciclosporin A is simple to operate, has high detection speed, uses less samples and has low cost, higher sensitivity and wider linear range compared with an ELISA (Enzyme-Linked Immuno Sorbent Assay) method, and good application prospect.

Description

A method for detecting plasma concentration of cyclosporin A by using competitive method technical field The invention belongs to the field of detection of cyclosporine A, in particular to a method for detecting blood drug concentration of cyclosporine A by using a competition method. Background technique Cyclosporin A (cyclosprine, CsA) is a cyclic polypeptide composed of 11 amino acids, which is an active metabolite of a fungus in soil. In 1978, CsA was first used in clinical kidney transplantation in the United Kingdom. Since then, CsA has been used in transplantation of liver, heart, lung, pancreas, bone marrow and other organs. Satisfactory results have been achieved, and the survival rate of patients has been significantly improved. Cyclosporin A entered my country in 1984, and a triple immunosuppressive regimen of "cyclosporin A + azathioprine + hormone" was formed, which greatly improved the survival rate of transplantation, and also greatly reduced the incidence o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/543
Inventor 孙庆莉
Owner SHANGHAI GENEXT MEDICAL TECH
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