Fluorescent quantitative PCR kit for quickly detecting human enterovirus 71
A technology of fluorescence quantification and enterovirus, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., which can solve the problems of false positives, low sensitivity, and inability to accurately quantify false positives.
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[0037] Further description will be given below in conjunction with specific embodiments.
[0038] It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of protection of the present invention.
[0039] Concrete experimental conditions and methods are not indicated in the following examples, usually according to conventional conditions such as: editors such as J. Sambrook, Science Press, 1992, Molecular Cloning Experiment Guide (Second Edition); D.L. Spector etc., Science Publishing House, 2001, Cell Experiment Guide; Lu Hongsheng, Science Press, 1982, or follow the conditions suggested by the manufacturer.
[0040] One, about the embodiment of preparation and detection method
[0041] ① Preparation of RNA extraction solution
[0042] That is TRIZOL nucleic acid extraction reagent.
[0043] ②Preparation of fluorescent quantitative PCR reaction solution
[0044] a. Composition of fluorescent PCR 1...
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