Fluorescent quantitative PCR kit for quickly detecting human enterovirus 71

Abstract

The invention discloses a fluorescent quantitative polymerase chain reaction (PCR) kit for quickly detecting human enterovirus 71, relates to a technology for detecting gene of pathogen causing various diseases, and is suitable for qualitative and quantitative detection of the human enterovirus 71. The kit comprises RNA extracting solution, fluorescent quantitative PCR reaction solution, an EV71 standard positive template PMD18-T-EV71 and a negative quality control standard, wherein the fluorescent quantitative PCR reaction solution contains a human enterovirus 71 specific primer pairs and a fluorescent probe. The kit is accurate in quantity, high in detection speed, and high in specificity and sensitivity, can identify and detect the human enterovirus 71, and is simple in using steps and high in repeatability. The kit can quickly perform qualitative and quantitative detection on the human enterovirus 71, and can replace the traditional culture method.

Description

technical field [0001] The invention relates to a gene detection technology for pathogens causing various human diseases, in particular to a rapid detection human enterovirus 71 fluorescent quantitative PCR kit, which is suitable for qualitative and quantitative detection of human enterovirus 71. Background technique [0002] Human enterovirus 71 (EV71) belongs to the genus Enterovirus of the family Picornaradae, and viruses of the same genus include poliovirus, coxsackievirus and echovirus. This type of virus spreads through the blood circulation after invading and multiplying in the digestive tract tissues of the human body, and eventually causes various clinical symptoms. EV71 was first isolated from the cerebrospinal fluid of a 9-month-old encephalitis child in California by Schmidt et al. in 1969, serotyped in 1972, and first reported in 1974. Since the report, the virus has caused more than a dozen outbreaks and epidemics around the world, and thus has received widesp...

AI Technical Summary

Problems solved by technology

[0005] The traditional culture method and serological method in clinical detection have the disadvantages of low sensitivity, poor specificity, false positive, time-consuming and labor-intensive; although the conventional PCR method has the advantages of simplicity, rapidity and sensitivity, it has the disadvantages of inaccurate quantification and PCR post-processing. False positives caused by pollution; therefore, it is necessary to develop an accurate, sensitive, rapid and pollution-free clinical testing method

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