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Selenium yeast for feeds and preparation method thereof

A technology for selenium yeast and feed, which is applied to the field of selenium yeast for feed and its preparation, can solve the problems of lack of large-scale production, unrealized production, lack of optimal conditions for selenium-enriched fermentation, etc., and achieves low cost and avoids the use of elemental selenium. The effect of generating and improving the conversion rate of selenium

Active Publication Date: 2012-12-12
INNER MONGOLIA YOURAN ANIMAL HUSBANDRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation of selenium yeast in my country is still in the laboratory research stage, and the actual production has not been realized. The relevant research still lacks the exploration of the optimal conditions for selenium-enriched fermentation, and does not have the guiding significance for large-scale production.

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  • Selenium yeast for feeds and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] Prepare the selenium yeast of the present embodiment according to the following method, see also figure 1 The flow shown:

[0036] Primary enrichment culture: Pick five rings of brewer's yeast on the inclined surface of the test tube and inoculate them in 300ml wort enrichment medium (the wort enrichment medium consists of 130g wort plus 1000ml distilled water, and the sugar content is adjusted to 8 waves Lin, adjust the pH to 6.0, sterilize at 121°C for 20 minutes, measure 300ml) in an Erlenmeyer flask (500ml capacity of the Erlenmeyer flask), plug the bottle stopper, and wrap it with a parafilm, at 30°C, 120 Shake for 24 hours under the condition of rev / min for primary enrichment culture;

[0037] Secondary enrichment culture: 50kg secondary seed culture medium (by 8 weight portions of corn flour, 2 weight portions of bran, nutrient salt (made by mixing potassium dihydrogen phosphate, ammonium sulfate, glucose according to 1.5:5:1) ) 0.75 parts by weight, add 39.25 ...

Embodiment 2

[0041] Prepare the selenium yeast of the present embodiment according to the following method:

[0042] Primary enrichment culture: Inoculate the brewer's yeast on the inclined surface of the test tube with a ratio of about 60ml per ring into the wort enrichment medium (add 7.5 times the volume of distilled water from the wort, adjust the sugar content to 6 Pauline, adjust the pH to 6.5, sterilized at 120°C for 25 minutes) in a Erlenmeyer flask, stoppered, wrapped with a parafilm, and shaken at 30°C and 120 rpm for 24 hours for primary enrichment culture ;

[0043] Secondary enrichment culture: 100kg secondary seed culture medium (by 18 weight portions of corn flour, 4 weight portions of bran, nutrient salt (made by mixing potassium dihydrogen phosphate, ammonium sulfate, glucose according to 1.5:5:1) ) 1.5 parts by weight, add 76.5 parts by weight of water and mix evenly, adjust the pH to 4.5, and sterilize at 121°C for 30 minutes to make) heat to 60°C, add 8U of α-amylase w...

Embodiment 3

[0047] Prepare the selenium yeast of the present embodiment according to the following method:

[0048] Primary enrichment culture: Inoculate the brewer's yeast on the inclined surface of the test tube in the ratio of 50ml per ring into the wort enrichment medium (add 8 times the volume of distilled water from the wort, adjust the sugar content to 10 Pauline, and adjust the pH to 6.0, made by sterilizing at 121°C for 20 minutes), stopper the bottle, wrap it with a parafilm, shake it at 30°C and 150 rpm for 28 hours for primary enrichment culture;

[0049] Secondary enrichment culture: 50kg secondary seed medium (by 15 parts by weight of corn flour, 5 parts by weight of bran, nutrient salt (made by mixing potassium dihydrogen phosphate, ammonium sulfate, glucose according to 1.5:5:1) ) 1.5 parts by weight, add 78.5 parts by weight of water and mix evenly, adjust the pH to 4.0, and sterilize at 121°C for 20 minutes to make) heat to 55°C, add 8U of α-amylase with enzyme activity ...

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Abstract

The invention relates to selenium yeast for feeds and a preparation method thereof. The preparation method of the selenium yeast for feeds comprises the following steps of: mixing beer yeast subjected to secondary liquid culture with a feed fermentation substrate and water in a weight ratio of (1-2) to (10-20) to (5-10); fermenting at 30-45 DEG C for 36-78h; and then, drying and crushing the fermented materials to obtain a finished selenium yeast product for feeds in the invention. The method provided by the invention is simple and easy to produce in batch. The selenium yeast prepared by utilizing the method of the invention contains organic selenium up to 500-1000 mg / kg, and the selenium product provided by the invention contains live yeast bacteria up to 2 billion / g and crude protein upto 35 percent, is safe and non-toxic and has less investment, rapid effect and high benefits.

Description

technical field [0001] The invention relates to a selenium yeast for feed and a preparation method thereof, in particular to a method for preparing selenium yeast for feed by selecting high-quality yeast strains and inorganic selenium sources and adopting liquid and solid-state combined fermentation technology, and the method according to the method Prepare the resulting feed with selenium yeast. Background technique [0002] The trace element selenium has the functions of participating in glutathione peroxidase, protecting human and animal organisms from free radicals and radiation damage, maintaining cellular respiration, stimulating immune responses, protecting the liver, and inhibiting cancer cells. In the production of traditional feed and feed additives, inorganic selenium—sodium selenite has been widely used as a supplement of trace element selenium. However, sodium selenite is highly toxic, and improper use or management will cause serious adverse consequences. Ther...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/16C12N1/18C12R1/865A23K10/16
Inventor 宋丽华韩吉雨孙晓智岳丽莎耿跃王福慧岳彦
Owner INNER MONGOLIA YOURAN ANIMAL HUSBANDRY CO LTD
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