Immunochromatography rapid kit and production method thereof

A technology of immunochromatographic test strips and analytical membranes, which can be used in analytical materials, biological tests, material inspection products, etc., and can solve problems such as intra-batch precision not higher than 15%.

Active Publication Date: 2011-07-06
BEIJING HOTGEN BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The development of immunochromatographic test strips for rapid detection of AFP and CRP by applying up-transfer luminescence immunoassay technology has clinical application value in the field of point-of-care rapid detection (POCT), but the intra-assay precision (CV) should not be higher than 15% to meet Clinical use needs

Method used

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  • Immunochromatography rapid kit and production method thereof
  • Immunochromatography rapid kit and production method thereof
  • Immunochromatography rapid kit and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: The difference between the drying method and the freeze-drying method in the preparation process of the immunochromatographic test strip based on the up-transition luminescence technology and its influence on CV:

[0039] A) contrast drying method and freeze-drying method difference (such as image 3 shown)

[0040] Preparation of freeze-dried binding pad: UCP particles were purchased from Shanghai Kerun Optoelectronics Technology Co., Ltd., glass fiber and cellulose film were purchased from Millipore, plastic cards and absorbent pads were purchased from Shanghai Jinbiao Biotechnology Co., Ltd., plastic cards were purchased from Shenzhen City Jincanhua Industrial Co., Ltd.

[0041] a) Centrifuge 50ml of UCP marker (UCP-biomolecule) suspension at 12000rpm at 4°C for 30min, and discard the supernatant;

[0042] b) Add 5ml of preservation solution (0.15M phosphate buffer solution with pH=7.2, containing 2wt% BSA and 0.05wt% NaN) to the UCP precipitate 3 ), pi...

Embodiment 2

[0058] Embodiment 2: Principle of AFP-UPT immunochromatography test strip and method for preparing AFP-UPT immunochromatography test strip by freeze-drying process

[0059] The principle of the test strip: AFP-UPT rapid detection test strip adopts double-antibody sandwich immunochromatography method to quantitatively detect the content of alpha-fetoprotein in the sample. The test area (T) on the membrane is pre-coated with anti-α-fetoprotein monoclonal antibody, the quality control area (C) is pre-coated with goat anti-mouse IgG, and another strain of anti-α-fetoprotein monoclonal antibody labeled with UCP particles on the binding pad. During the reaction, the alpha-fetoprotein in the sample reacts with the two antibodies to form an anti-alpha-fetoprotein antibody-alpha-fetoprotein antigen-UCP-labeled anti-alpha-fetoprotein antibody complex in the T area. UCP particles emit visible light under infrared light excitation. The intensity of the emitted light is directly proportion...

Embodiment 3

[0086] Embodiment 3. adopt the composition of the AFP-UPT kit that lyophilization method is produced:

[0087] 1. The AFP-UPT immunochromatographic test strip prepared in Example 2

[0088] 2. Sample diluent

[0089] 3. Sample tube

[0090] 4. Instruction manual

[0091] The composition of the sample diluent in the AFP-UPT kit is as follows: 0.15M phosphate buffer solution with pH=7.2, containing 1% v / v Tween 20.

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Abstract

The invention relates to a production method of immunochromatography test paper, and alpha-fetoprotein fast quantitative immunochromatography test paper and C reactive protein fast quantitative immunochromatography test paper both prepared by using the production method. The immunochromatography test paper uses UCP (Up-Converting Phosphor) as biomarker. The structure consists mainly of: a sample cushion, a binding cushion or a bonder dilution cushion, a cellulose nitrate film, a water absorption cushion and a plastic card. The invention relates to the method for the industrial production of such up-converting immunochromatography test paper and the using method thereof. By using the up-converting immunochromatography test paper, the content of a detected material can be determined rapidly, conveniently and quantitatively, and support can be offered to the prevention, diagnosis and treatment of disease accurately.

Description

technical field [0001] The invention relates to an immunochromatographic test strip, a preparation method thereof and a kit containing the test strip. Specifically, the present invention relates to an immunochromatographic test strip based on upconversion luminescence technology, a production method for producing the immunochromatographic test strip by lyophilization, and an immunochromatographic test strip comprising the described immunochromatographic test strip. Detection kit. The present invention also relates to an immunochromatographic test strip and a test kit for detecting alpha-fetoprotein and C-reactive protein produced by the above-mentioned production method. Background technique [0002] Chinese patent application 200410034104.0 describes a substance called an upconverting luminescent material (UCP) and an immunochromatographic test strip using the material as a marker. [0003] Although up-converting luminescent materials have many advantages such as high sen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/52G01N33/531G01N33/68
Inventor 林长青杨瑞馥周蕾李华柏
Owner BEIJING HOTGEN BIOTECH CO LTD
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