Application of arachidonic acid as xanthine oxidase inhibitor and in preparation of medicines for treating hyperuricemia or gout

Abstract

The invention relates to application of arachidonic acid as a xanthine oxidase inhibitor and in the preparation of medicines for treating hyperuricemia or gout. Xanthine oxidase is a key enzyme for the generation and the development of the hyperuricemia or the gout, most substrates of the xanthine oxidase are cyclic small molecules, and the arachidonic acid is long chain polyunsaturated fatty acid; a three dimensional structure of the xanthine oxidase is downloaded from a protein data bank (PDB) of a website; a three dimensional structure of the arachidonic acid is downloaded from a pubchem; molecular docking between the arachidonic acid and the xanthine oxidase is performed by iGEMDOCK software and docking energy is computed; and in-vitro enzyme activity tests prove that the arachidonic acid has an inhibition effect on the xanthine oxidase. By the application, another important physiological function of the arachidonic acid is discovered, and a wider way is provided for screening the xanthine oxidase inhibitor from a natural product to treat the hyperuricemia or the gout simultaneously.

Description

technical field [0001] The invention belongs to the field of natural product xanthine oxidase inhibitors, in particular to the use of arachidonic acid as a xanthine oxidase inhibitor and its application in the preparation of medicines for treating hyperuricemia or gout. Background technique [0002] Arachidonic acid (AA), whose system name is 5,8,11,14-trans-eicosatetraenoic acid, is an important polyunsaturated fatty acid of the omega-6 series in the human body. Since arachidonic acid is many eicosan derivatives such as prostaglandin E 2 , prostacyclin, thromboxane A2 and leukotrienes and other substances precursors, therefore, arachidonic acid has many important health functions, such as intelligence and brain, improve visual acuity, esterify cholesterol, inhibit platelet aggregation, Increase blood vessel elasticity, reduce blood viscosity, regulate blood cell function, regulate blood lipid and blood sugar, protect skin, anti-inflammation, etc. As a natural component of...

AI Technical Summary

Problems solved by technology

[0005] Xanthine oxidase is a non-specific enzyme, and many compounds can be used as its substrates, among which hypoxanthine and xanthine are the best substrates, and their structures are very different from arachidonic acid, which is long-chain aliphatic hydrocarbons, and most of the substrates of xanthine oxidase are small molecules containing benzene rings, which is why no studies have considered the inhibitory effect of arachidonic acid on xanthine oxidase

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