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Leech freeze-dried powder injection solution for injection and preparation method thereof

A technology for freeze-dried powder injection and water for injection, which is applied in freeze-dried delivery, medical preparations containing active ingredients, powder delivery, etc., can solve the problems of short stabilization time, restricted use, etc. Avoid easily degradable effects

Inactive Publication Date: 2011-08-03
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a hospital preparation, leech injection has been used in many large hospitals for many years, but because its active ingredients are peptides, the stability time in water is short, and it needs to be prepared temporarily before use, which greatly limits its clinical use

Method used

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  • Leech freeze-dried powder injection solution for injection and preparation method thereof
  • Leech freeze-dried powder injection solution for injection and preparation method thereof
  • Leech freeze-dried powder injection solution for injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A leech freeze-dried powder for injection, the preparation of which comprises the following steps:

[0020] (1) Preparation of leech polypeptide powder:

[0021] Take 2.0 kg of dried medicinal materials of leeches, grind them into powder, put the powder in a container with a cover, soak and extract with 20 times the volume of 40% acetone at 0-4°C for 4 times, combine the extracts, recover the acetone, and reduce it at 40°C. Concentrate under pressure to a relative density of 1.05. Add ethanol to the concentrated solution until the final concentration of ethanol is 60%, stir well, stand overnight at 0-4°C, filter, discard the precipitate, concentrate the mother liquor at 40°C under reduced pressure to a relative density of 1.05, and add ethanol again to make The final concentration of ethanol is 85%, stir well, let stand overnight at 0-4°C, and filter. The precipitate was collected and vacuum-dried until the precipitate was free of ethanol and water to obtain 100.5 g o...

Embodiment 2

[0027] A leech freeze-dried powder for injection, the preparation of which comprises the following steps:

[0028] (1) Preparation of leech polypeptide powder:

[0029] Take 2.3 kg of dried Japanese medicine leech, grind it into powder, put the powder in a container with a cover, soak and extract with 30 times the volume of 50% ethanol at 20°C for 4 times, combine the extracts, and concentrate under reduced pressure at 40°C to The relative density is 1.08. Add ethanol to the concentrated solution until the final concentration of ethanol is 60%, stir well, let stand overnight at 20°C, filter, discard the precipitate, concentrate the mother liquor at 40°C under reduced pressure to a relative density of 1.08, add ethanol again, and make the concentration of ethanol The final concentration is 85%, stir well, let stand overnight at 20°C, and filter. The precipitate was collected and vacuum-dried until the precipitate was free of ethanol and water to obtain 108.5 grams of leech po...

Embodiment 3

[0035] A leech freeze-dried powder for injection, the preparation of which comprises the following steps:

[0036] (1) Preparation of leech polypeptide powder:

[0037] Take 2.2 kg of dry medicinal materials of willow leaf leech, grind it into powder, put the powder in a container with a cover, use 40 times the volume of 30% acetone, extract by leakage at 20°C, combine the extracts, concentrate under reduced pressure at 40°C to relative density to 1.10, add ethanol until the final concentration of ethanol is 60%, stir well, stand overnight at 20°C, filter, discard the precipitate, concentrate the mother liquor at 40°C under reduced pressure to a relative density of 1.10, add ethanol again to make the final concentration of ethanol The concentration is 85%, stir well, stand at 20°C overnight, and filter. The precipitate was collected and vacuum-dried until the precipitate was free of ethanol and water to obtain 105.5 grams of leech polypeptide powder.

[0038] (2) Preparation...

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Abstract

The invention discloses leech freeze-dried powder injection solution for injection and a preparation method thereof. A leech medicinal material recorded in Chinese pharmacopeia is used as a raw material, and the preparation method comprises the following steps of: crushing the leech medicinal material; performing soaking extraction and seeping extraction by using 0 to 80 percent acetone / acetone aqueous solution or ethanol / ethanol aqueous solution in an amount which is 1 to 100 times the raw materials at the temperature of between 0 and 80 DEG C; concentrating extracting solution under reduced pressure; precipitating with 60 percent ethanol to remove impurities; concentrating mother liquor under reduced pressure; precipitating with 85 percent ethanol; precipitating and performing vacuum drying to obtain leech polypeptide powder; and compounding the leech polypeptide powder, a freeze-dried powder proppant and an osmotic pressure regulator to obtain the powder injection solution by a freezing-drying technology. The preparation is mainly used for preventing and treating cardiovascular and cerebrovascular diseases. Compared with hospital preparations of leech injection solution and market-sold formula solid preparations, the powder injection solution has the advantages of obvious activity, high stability, long validity and the like, and is convenient to store and transport.

Description

technical field [0001] The invention relates to a leech freeze-dried powder injection for injection, and also relates to a preparation method of the powder injection. Background technique [0002] Cardiovascular and cerebrovascular diseases are one of the main diseases that endanger human health, accounting for one-third of the global death factors, and there is an increasing trend. At present, my country is gradually entering an aging society, and the demand for anticoagulant drugs is increasing. However, both traditional urea kinase and heparin have the disadvantage of bleeding after administration, which limits their clinical application. [0003] Leeches, first recorded in "Shen Nong's Materia Medica", the 2010 edition of the "Pharmacopoeia of the People's Republic of China", the leech medicinal materials recorded are the dried bodies of leech Whitmania pigra, Japanese medical leech Hirudo nipponica and willow leaf leech Whitmania acranulatc. It tastes bitter and has the...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K35/62A61K9/19A61P7/02
Inventor 钟山杨得坡
Owner SUN YAT SEN UNIV
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