Extraction and biological activity modification method for salicornia polysaccharide
A technology of active modification and samphire, applied in fermentation and other directions, can solve the problem of lack of research on biomodification technology of samphire polysaccharide molecules, and achieve the effect of increasing farmers' income
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Embodiment 1
[0010] The purification of embodiment 1 salicornia polysaccharide
[0011] Studies have shown that the TCA with a concentration of 2-6% is used to remove proteins, and the ethanol solution with a concentration of 50-85% is used to precipitate polysaccharides; under these conditions, the yield of polysaccharides can reach 3-4.5%, and the purity can reach 50-60%. Trichloroacetic acid combined with microfiltration (0.1 μm) technology can make the protein clearance rate reach 80-85%, which is 50-60% higher than that of trichloroacetic acid only under the same conditions, and the polysaccharide loss rate is only 4-6%. Through the screening of the pore size of the ultrafiltration membrane, membranes with molecular weight cut-offs of 30kDa and 1kDa were selected. The process parameters in the ultrafiltration process are determined, respectively, the operating pressure is 8-12psi, the temperature is 30-45°C, and the time is 1-2.5h. The application of ultrafiltration technology will r...
Embodiment 2
[0012] The biological activity research of embodiment 2 polysaccharide
[0013] Animal experiments were used to evaluate the effect of Salicornia polysaccharide on enhancing immune function. According to 0.4ml / 20g BW -1 The amount of intragastric administration to mice 75mg mL -1 , 50mg mL -1 and 25mg mL -1 Three doses of salicornia polysaccharide solution were carried out continuously for 30 days. The body weight, relative organ weight, delayed type hypersensitivity (DTH), serum lectin anti-volume and phagocytosis of each experimental group were measured. The results showed that at 75mg mL -1 and 50mg mL -1 Under the dosage of , the phagocytosis index, phagocytosis rate and delayed type hypersensitivity (DTH), anti-volume number of mice are compared with control, difference is very significant (P-1 Under the dosage of , mice only had significant differences (P<0.05) compared with controls in non-specific immunity and humoral immunity (that is, in phagocytosis index, pha...
Embodiment 3
[0015] The biomodification of embodiment 3 polysaccharides
[0016] The results showed that lipase could not only efficiently catalyze the acylation reaction of salicornia polysaccharide in non-aqueous medium and many special reaction systems, but also had high stability and selectivity. Enzymes from different sources can catalyze the regioselective acylation of hydroxyl groups at different sites in polysaccharide compounds; the same enzyme exhibits different selectivities in different reaction media.
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