Extraction and biological activity modification method for salicornia polysaccharide

A technology of active modification and samphire, applied in fermentation and other directions, can solve the problem of lack of research on biomodification technology of samphire polysaccharide molecules, and achieve the effect of increasing farmers' income

Inactive Publication Date: 2011-09-07
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no complete set of high-efficiency separation and purification technology and method of Salicornia polysaccharide in China, and there is a lack of research on molecular biomodification technology of Salicornia polysaccharide. Therefore, how to transform the deep-processed products of Salicornia to high value-added comprehensive utilization is a key issue. The Important Direction of Salicornia Vegetable Industry Development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] The purification of embodiment 1 salicornia polysaccharide

[0011] Studies have shown that the TCA with a concentration of 2-6% is used to remove proteins, and the ethanol solution with a concentration of 50-85% is used to precipitate polysaccharides; under these conditions, the yield of polysaccharides can reach 3-4.5%, and the purity can reach 50-60%. Trichloroacetic acid combined with microfiltration (0.1 μm) technology can make the protein clearance rate reach 80-85%, which is 50-60% higher than that of trichloroacetic acid only under the same conditions, and the polysaccharide loss rate is only 4-6%. Through the screening of the pore size of the ultrafiltration membrane, membranes with molecular weight cut-offs of 30kDa and 1kDa were selected. The process parameters in the ultrafiltration process are determined, respectively, the operating pressure is 8-12psi, the temperature is 30-45°C, and the time is 1-2.5h. The application of ultrafiltration technology will r...

Embodiment 2

[0012] The biological activity research of embodiment 2 polysaccharide

[0013] Animal experiments were used to evaluate the effect of Salicornia polysaccharide on enhancing immune function. According to 0.4ml / 20g BW -1 The amount of intragastric administration to mice 75mg mL -1 , 50mg mL -1 and 25mg mL -1 Three doses of salicornia polysaccharide solution were carried out continuously for 30 days. The body weight, relative organ weight, delayed type hypersensitivity (DTH), serum lectin anti-volume and phagocytosis of each experimental group were measured. The results showed that at 75mg mL -1 and 50mg mL -1 Under the dosage of , the phagocytosis index, phagocytosis rate and delayed type hypersensitivity (DTH), anti-volume number of mice are compared with control, difference is very significant (P-1 Under the dosage of , mice only had significant differences (P<0.05) compared with controls in non-specific immunity and humoral immunity (that is, in phagocytosis index, pha...

Embodiment 3

[0015] The biomodification of embodiment 3 polysaccharides

[0016] The results showed that lipase could not only efficiently catalyze the acylation reaction of salicornia polysaccharide in non-aqueous medium and many special reaction systems, but also had high stability and selectivity. Enzymes from different sources can catalyze the regioselective acylation of hydroxyl groups at different sites in polysaccharide compounds; the same enzyme exhibits different selectivities in different reaction media.

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Abstract

The invention relates to an extraction separation and activity modification method of salicornia polysaccharide. The salicornia described in the invention comprises fresh plant and young plant stems, and the method comprises the following steps of: pulping or drying and pulverizing the salicornia, extracting the salicornia polysaccharide by an ultrasonic-assisted enzyme method; separating and purifying the salicornia polysaccharide by a membrane separation technology; analyzing the molecular weight and dispersion degree of the polysaccharide by gel filtration chromatography (GFC); determining the composition and proportion of monosaccharide by a gas chromatogram (GC) method; and modifying the polysaccharide by enzyme controlled degradation and enzymatic acylation. The invention lays a foundation for preparing high-activity salicornia polysaccharide and related products thereof.

Description

technical field [0001] The invention relates to the application of salicornia in the preparation of bioactive substances. Background technique [0002] Polysaccharides are biologically active substances that have been hotly studied in recent years. They can significantly regulate the immune function of the body and inhibit tumor cells. At the same time, they have no toxic and side effects on normal cells. In recent years, the development and utilization of coastal tidal flats, salt wastelands and seawater resources has become a major issue facing human existence, and has attracted widespread attention worldwide. As a result, saline soil agriculture and seawater irrigation agriculture have gradually emerged worldwide. Salicornia is a typical product of saline soil agriculture. Our country is very rich in salicornia resources. Salicornia polysaccharides are the most promising physiologically active substances in Salicornia. Salicornia polysaccharides have a unique ability to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C12S3/02C12P19/04
Inventor 余晓红邵荣许琦陈洪兴张春银许伟
Owner YANCHENG INST OF TECH
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