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Prodenia litura genetically engineered virus NO.2 and construction method thereof

A technology of genetic engineering and Spodoptera litura is applied in the field of microorganisms to achieve the effects of low dose effect, less resistance to pesticides, and fast insecticidal speed

Inactive Publication Date: 2013-05-15
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there have been no reports on the successful construction of the genetically engineered virus of Spodoptera litura nucleopolyhedron at home and abroad

Method used

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  • Prodenia litura genetically engineered virus NO.2 and construction method thereof
  • Prodenia litura genetically engineered virus NO.2 and construction method thereof
  • Prodenia litura genetically engineered virus NO.2 and construction method thereof

Examples

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Embodiment 1

[0025] The present invention, through the method of genetic engineering, has strong virulence wild virus SpltMNPV ( Spodoptera litur a nucleopolyhedrovirus , GenBank accession number: NC-011616 ) on the basis of deletion and recombination, a genetically engineered virus of Spodoptera litura was provided, and a recombinant Spodoptera litura nuclear polyhedrosis virus was constructed, i.e. Spodoptera litura genetically engineered virus No. 2 (SpltMNPV -△ egt - BmK IT 1- egfp ) CCTCC NO: V201028.

[0026] See attached figure 1 , it is a kind of Spodoptera litura genetically engineered virus provided in this embodiment, namely the structural representation of No. 2 Spodoptera litura genetically engineered virus; Among the figure, egt is the ecdysone uridine diphosphate glucosyltransferase gene; P pH is the polyhedrin gene promoter; BmK IT 1 is the East Asian pincer venom gene; egfp is the enhanced green fluorescent protein gene; SignalP for SpltMNPV F prote...

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Abstract

The invention relates to the field of a microorganism technology, in particular relating to a prodenia litura genetically engineered virus which can prevent farming and forestry pests such as prodenia litura. The virus is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: V201028. In the invention, a genetic engineering method is employed to delete and restructure a wild type virus. The egt gene, namely the ecdysone uridine diphosphate glucosyltransferase gene is deleted from the genome of the virus; a BmKTal gene controlled by a wild type virus polyhedron gene (ph) promoter and a marking gene-enhanced green fluorescent protein (egfp) gene controlled by the wild type virus polyhedron gene (ph) promoter are inserted into the locus at which the egtgene is deleted. The virus provided by the invention has the advantages of faster pest killing speed than that of the wild type virus, less dosage effect, better field application effect and the like.

Description

technical field [0001] The present invention relates to the technical field of microbes, in particular to a genetically engineered virus of Spodoptera litura which can prevent and control agricultural and forestry pests such as Spodoptera litura, i.e. "Spodoptera litura Genetic Engineering Virus No. 2" (SpltMNPV-△ egt - BmK IT 1- egfp ), the taxonomic name is "Spodoptera litura nuclear polyhedrosis virus genetic engineering strain No. 2" (Genetically modified Spodoptera litur a nucleopolyhedrovirus No.2). The virus was preserved by the China Center for Type Culture Collection (CCTCC), the preservation date is December 15, 2010, and the preservation number is CCTCC NO: V201028. Background technique [0002] Spodoptera litura is one of the main pests of crops, and it is distributed in various provinces and regions in China. The insectivorous nature is extremely wide, and the host plants known to be harmful are more than 290 kinds in 99 families. In recent years, with t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/85A01P7/04C12R1/93
Inventor 朱江浦冠勤郭大磊王文兵汤欣欣孙兴鲁秦启联
Owner SUZHOU UNIV
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