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Molecular target for diagnosing and treating nasopharyngeal cancer related with Epstein-Barr virus (EBV) infection and application thereof

A molecular target, nasopharyngeal cancer technology, applied in the field of molecular targets, can solve the problem of little understanding of miRNA

Inactive Publication Date: 2011-09-07
CENT SOUTH UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Regarding the relationship between EBV, miRNA and related tumors, there is no research report to confirm it, and the miRNAs directly affected by EBV are not known so far
The role of Hsa-miR203 in NPC and whether it is related to the role of EBV and the mechanism have not been reported

Method used

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  • Molecular target for diagnosing and treating nasopharyngeal cancer related with Epstein-Barr virus (EBV) infection and application thereof
  • Molecular target for diagnosing and treating nasopharyngeal cancer related with Epstein-Barr virus (EBV) infection and application thereof
  • Molecular target for diagnosing and treating nasopharyngeal cancer related with Epstein-Barr virus (EBV) infection and application thereof

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Embodiment Construction

[0026] The following in conjunction with specific embodiments is intended to further illustrate the present invention, rather than limit the present invention.

[0027] (1) Perform microRNA chip screening on the 293-EBV cell line with stable expression of EBV constructed earlier and its control group, and determine the target: hsa-miR203; the sequence is shown in sequence 1 in the sequence list, and the sequence can be seen in miRBase: the microRNA database; http: / / www.mirbase.org / .

[0028](2) 293-EBV cells stably infected by EBV (i.e. HEK293 stably transfected EBV virus genome cells), 293-blank control cell line, 293 cell line (i.e. HEK293: human embryonic kidney epithelial cells), Lm cell line (293- EBV cell line cultured at low density (EBV lost cell clones) were cultured in DMEM medium with 10% fetal bovine serum. Four lines of EBV immortalized lymphoblastoid cells [E33, E20, E18, E14], Raji cells (EBV positive lymphoma cells), B95-8 cells (EBV positive marmoset lymphocy...

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Abstract

The invention discloses a molecular target hsa-miR203 for diagnosing and treating nasopharyngeal cancer related with Epstein-Barr virus (EBV) infection and application thereof. The microRNA can accurately reflect the conditions of retention and loss of EBV viruses in cells and tissues, and further can be used for diagnosing the nasopharyngeal cancer related with the EBV infection; and meanwhile, target genes E2F3, CCNG1 and the like controlled by the hsa-miR203 obviously inhibit the proliferation and conversion capacities of the cells, so the hsa-miR203 can be used for treating the nasopharyngeal cancer related with the EBV infection. Therefore, the hsa-miR203 has important scientific research theory and clinical application value, and provides new clue and proof for diagnosis, treatment or prognosis of EBV related tumors.

Description

technical field [0001] The invention belongs to the technical field of molecular targets, and relates to molecular targets for diagnosis and treatment of nasopharyngeal carcinoma related to EBV infection and applications thereof. Background technique [0002] According to the International Agency for Research on Cancer's classification criteria for carcinogens, Epstein-Barr virus is listed in the first group of carcinogens. EBV is closely related to the high incidence of nasopharyngeal carcinoma (NPC) in southern China. In NPC tissues, almost all poorly differentiated tumor cells contained EBV genomes. The existence of EBV genome plays an important role in the occurrence and development of related tumors. Some scholars believe that the environment of tumor tissue, such as the virus in it, will cause abnormal changes in the expression profile of tumor cell miRNA, which can be used to explain the important reason why some viruses are highly related to tumors. Regarding the ...

Claims

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Application Information

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IPC IPC(8): C12N15/113C12Q1/70C12Q1/68A61K48/00A61P31/22A61P35/00C12R1/93
Inventor 李桂源俞海波卢建红赵莲李小玲唐海林喻正源李夏雨
Owner CENT SOUTH UNIV
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