Preparation method for cerebroprotein hydrolysate in piracetam cerebroprotein hydrolysate tablets

A cerebroprotein hydrolysate and proteolytic enzyme technology, applied in the field of biological preparations, can solve problems such as adverse reactions and inconvenience of injections, and achieve the effect of improving energy metabolism

Inactive Publication Date: 2011-09-21
HONGMEI PHARMA CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to some inconvenience, the application of injections also has many adverse reactions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 10Kg of fresh and healthy pig brain, add 10L of water for injection, use a high-pressure homogenizer pump (GRB1500-4S, Shanghai Donghua high-pressure homogenate), freeze at -35°C, thaw at 15°C after freezing, and heat Keep at 80°C for 15 minutes, then cool down naturally to room temperature, centrifuge in a high-speed refrigerated centrifuge at 4°C at a speed of 10,000 rpm for 30 minutes, then separate the supernatant and precipitate, and set aside the supernatant;

[0036] Add 0.5% trypsin [Trypsin, 1:250, Amresco 0458] of the weight of the precipitate to the precipitate, perform enzymatic hydrolysis at 37°C for 4 hours, and control the pH value of the enzymatic hydrolysis to 8.0, then add the aforementioned supernatant, and adjust the pH value to 3.5, heat to 80°C, keep it for 15 minutes, cool down to room temperature naturally, centrifuge in a low-speed refrigerated centrifuge at 4,000 rpm for 25 minutes at 4°C, then filter the supernatant, and adjust the pH of t...

Embodiment 2

[0038] Take 10Kg of fresh and healthy dog ​​brain, add 10L of water for injection, homogenate under high pressure, freeze at -35°C, thaw at 20°C after freezing, heat to 85°C, keep for 15 minutes, then cool down to room temperature naturally, Centrifuge in a high-speed refrigerated centrifuge at a speed of 10,000 rpm for 25 minutes at ℃, then separate the supernatant and the precipitate, and the supernatant is set aside;

[0039] Add 0.3% trypsin [Trypsin, 1:250, Amresco 0458] of precipitation weight to the precipitation, enzymolysis at 40 ℃ for 4 hours, the enzymolysis pH value is controlled at 8.0, then add the aforementioned supernatant, adjust the pH value to 3.0, heat to 85°C, keep it for 20 minutes, cool down to room temperature naturally, centrifuge in a low-speed refrigerated centrifuge at a speed of 3500 rpm at 2°C for 30 minutes, then filter the supernatant, and adjust the pH of the filtrate to 7.0, heated to 85°C, kept for 30 minutes, cooled down to room temperature ...

Embodiment 3

[0041] Take 10Kg of fresh and healthy bovine brain, add 15L of water for injection, homogenate under high pressure, freeze at -30°C, thaw at 30°C after freezing, heat to 90°C, keep for 30 minutes, then cool down to room temperature naturally, Centrifuge in a high-speed refrigerated centrifuge at a speed of 11,000 rpm for 20 minutes at ℃, then separate the supernatant and the precipitate, and the supernatant is set aside;

[0042] Add 0.1% trypsin [Trypsin, 1:250, Amresco 0458] of the weight of the precipitate to the precipitate, perform enzymatic hydrolysis at 45°C for 6 hours, and control the pH value of the enzymatic hydrolysis to 7.5, then add the aforementioned supernatant, and adjust the pH value to 3.5, heat to 90°C, keep it for 25 minutes, cool down to room temperature naturally, centrifuge in a low-speed refrigerated centrifuge at a speed of 3500 rpm at 1°C for 25 minutes, then filter the supernatant, and adjust the pH of the filtrate to 7.5, heat to 90°C, keep it for ...

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Abstract

The invention provides a preparation method for cerebroprotein hydrolysate in piracetam cerebroprotein hydrolysate tablets. The cerebroprotein hydrolysate is extracted from brain tissues of mammals except for human being and contains specific polypeptide and various amino acids including free lysine and free glutamic acid, and molecular weight of all compositions is below 5,000-10,000 daltons. The weight proportion of polypeptide to free lysine to free glutamic acid in the cerebroprotein hydrolysate is (1-200):(0.1-100):(0.1-80). According to the invention, the pharmaceutical composition contains active substances capable of treating cranial vascular disease and cerebral metabolism disorder sequela caused thereby, the active substances can regulate and improve cerebral metabolism. After determination, the content of amino nitrogen in the cerebroprotein hydrolysate achieves 40-50 percent of total nitrogen content, so that the cerebroprotein hydrolysate can be used for preparing compound piracetam cerebroprotein hydrolysate tablets.

Description

technical field [0001] The present invention relates to a preparation method of cerebroprotein hydrolyzate in piracetam cerebroprotein hydrolyzate sheet, in particular, relates to a kind of cerebroprotein hydrolyzate extracted from the brain tissue of mammals other than human, containing specific polypeptide and including free The brain protein hydrolyzate of multiple amino acids including lysine and free glutamic acid belongs to the field of biological preparations. Background technique [0002] The content of free amino acids in brain tissue is highest with glutamic acid, followed by taurine, and then aspartic acid. Glutamate is the main excitatory neurotransmitter in the brain. Brain injury can lead to an ion imbalance in brain tissue, triggering an excitotoxic cascade involving glutamate and other excitatory amino acids, and leading to neuronal death in brain tissue surrounding the initial site of injury. Lysine is one of the eight essential amino acids. It plays an im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/30A61K38/02A61P9/10A61P25/00A61P25/28A61P25/18
Inventor 郭智华
Owner HONGMEI PHARMA CHINA
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