Yeast and method for producing erythritol by multistage yeast fermentation

An erythritol, multi-stage technology, applied in the field of high-yielding erythritol-producing yeasts, can solve the problems of long fermentation cycle, many extraction and separation steps, inhibition, etc., and achieve low cost, high yield and improved yield Effect

Inactive Publication Date: 2012-09-19
NANJING XINBAI PHARMA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the problems of long fermentation period, multiple extraction and separation steps, and high-concentration product substrate inhibition in the production of erythritol by fermentation at home and abroad, if a systemic multi-stage reaction and The basic theory of separation and coupling, especially in-depth research on the separation and extraction of products, will be of great significance

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Yeast and method for producing erythritol by multistage yeast fermentation
  • Yeast and method for producing erythritol by multistage yeast fermentation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0042] ①Cultivation of seeds

[0043] Pick 1 yeast Saccharomyces cerevisiae NJWGYH30566 (CGMCCNo.4349) colony from the PDA plate into a 500mL Erlenmeyer flask filled with 25mL of seed medium, and cultivate it in a shaking table with a temperature of 25°C, a pH of 5.5, and a rotation speed of 150rpm for 40h. Obtain seed culture fluid;

[0044] The composition of the above seed medium is (g / L): 150 glucose, 10 yeast extract, and 3 corn steep liquor.

[0045] ② Preparation before inoculation

[0046] Clean the fermenter, assemble the thermometer, pH meter (corrected) and foam sensor, dissolve the fermentation medium in water, stir evenly, pour it into the fermenter, put in cooling water, sterilize, and correct the dissolved oxygen before sterilization DO is 0%, connect the air filter, and connect other ancillary equipment after cooling.

[0047] The composition of its fermentation medium is (g / L): glucose 200, yeast extract 20, corn steep liquor 10, MgSO 4 ·7H 2 O0.3, MnCl ...

example 2

[0056] ①Cultivation of seeds

[0057] Pick 1 yeast Saccharomyces cerevisiae NJWGYH30566 (CGMCCNo.4349) colony from the PDA plate, put it in a 500mL Erlenmeyer flask filled with 40mL of seed medium, and cultivate it in a shaker with a temperature of 30°C, a pH of 6.5, and a rotation speed of 180rpm for 36h. Obtain seed culture fluid;

[0058] The composition of the above seed medium is (g / L): 200 glucose, 16 yeast extract, and 4 corn steep liquor.

[0059] ② Preparation before inoculation

[0060] Clean the fermenter, assemble the thermometer, pH meter (corrected) and foam sensor, dissolve the fermentation medium in water, stir evenly, pour it into the fermenter, put in cooling water, sterilize, and correct the dissolved oxygen before sterilization DO is 0%, connect the air filter, and connect other ancillary equipment after cooling.

[0061] The composition of its fermentation medium is (g / L): glucose 250, yeast extract 25, corn steep liquor 15, MgSO 4 ·7H 2 O0.4, MnCl 2...

example 3

[0070] ①Cultivation of seeds

[0071] Pick 2 yeast Saccharomyces cerevisiae NJWGYH30566 (CGMCCNo.4349) colonies from the PDA plate and put them in a 500mL Erlenmeyer flask filled with 50mL of seed medium, and cultivate them in a shaker with a temperature of 35°C, a pH of 7.5, and a rotation speed of 200rpm for 30h. Obtain seed culture fluid;

[0072] The composition of the above-mentioned seed medium is (g / L): 250 glucose, 20 yeast extract, and 5 corn steep liquor.

[0073] ② Preparation before inoculation

[0074] Clean the fermenter, assemble the thermometer, pH meter (corrected) and foam sensor, dissolve the fermentation medium in water, stir evenly, pour it into the fermenter, put in cooling water, sterilize, and correct the dissolved oxygen before sterilization DO is 0%, connect the air filter, and connect other ancillary equipment after cooling.

[0075] The composition of its fermentation medium is (g / L): glucose 300, yeast extract 30, corn steep liquor 20, MgSO 4 ·...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a yeast, which is named as saccharomyces cerevisiae according to its classification. The Latin name of the strain is Saccharomyces cerevisiae; microbe by reference is NJWGYH30566; the preservation date is Nov. 15, 2010; and the collection center registration number is CGMCC No. 4349. The invention also provides a method for producing erythritol by multistage yeast fermentation, comprising seed culturing, pre-inoculation preparation and inoculation, multistage dissolved oxygen controlling, and fed-batch fermentation phase. The output of the primary metabolite erythritolis obviously raised by multistage fermentation. The method provided by the invention has advantages of low cost, simple operation and high output of erythritol in the fermentation broth, and is in favor of industrial production.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a multi-stage fermentation production method of yeast Saccharomyces cerevisiae NJWGYH30566, which produces high-yield erythritol. Background technique [0002] Erythritol (Erythritol) is 1,2,3,4-butanetetraol, the molecular formula is C 4 h 10 o 4 , is widely distributed in nature, and is contained in lichens, seaweeds, mushrooms and various plant fruits. Erythritol is a new type of nutritional sweetener, which is characterized by good thermal stability, low hygroscopicity and low freezing point. It is widely used in food, medicine, cosmetics, chemical industry and other fields. Its production methods mainly include chemical method and microbial fermentation method. The chemical method is to use periodate method to generate double whole starch from starch, and then hydrogenate and crack it into erythritol and other derivatives. The process is long ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12P19/02C12R1/865
Inventor 杨文革胡永红李玲
Owner NANJING XINBAI PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap