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Application of gene EIN3 and gene EIL1 in promoting growth of plant root hair

A plant, root hair technology, applied in the field of genetic engineering

Inactive Publication Date: 2011-10-19
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first green revolution proposed in the middle of the last century, a large amount of pesticides and chemical fertilizers were used in agricultural production. Although the yield of crops was improved, it brought very serious problems

Method used

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  • Application of gene EIN3 and gene EIL1 in promoting growth of plant root hair
  • Application of gene EIN3 and gene EIL1 in promoting growth of plant root hair
  • Application of gene EIN3 and gene EIL1 in promoting growth of plant root hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 EIN3 and EIL1 are important transcription factors regulating root hair growth

[0017] CTR1 is a negative regulator in the ethylene signaling pathway. When there is no ethylene, CTR1 shuts down the downstream ethylene response. After Arabidopsis senses the ethylene signal, it will turn off the downstream inhibitory function of CTR1. EIN2 is a positive regulator in the ethylene signaling pathway, which can promote the accumulation of transcription factors EIN3 and EIL1, which transcribe various ethylene-responsive genes. The ethylene-insensitive mutants ein2-5 and ein3 eil1, the constitutive mutant ctr1-1, and the hypersensitive mutant EIN3ox were sown on MS (purchased from PTL) and ACC (ethylene synthesis precursor, 10 μM) medium, respectively. After imbibition at 4°C for 3 days, place them in a long-day greenhouse at 22°C and culture them vertically for 6 days to observe the phenotype. It was found that the mutant ctr1-1, which constitutively turns on the et...

Embodiment 2

[0018] Example 2 EIN3 and EIL1 integrate internal and external signals that stimulate root hair growth

[0019] Iron deficiency is known to induce root hair growth, and treatment with the hormone ABA, which mimics drought stress, can also promote root hair development. A. Sow ein3 eil1 on MS medium, culture vertically under light for 3 days after imbibition, then transfer the seedlings to MS medium supplemented with 3 μM ABA, and continue to culture under light for 3 days to observe the phenotype. B. Sow the same seeds on MS' medium (5mM KNO 3 , 2mM MgSO 4 , 2mM Ca(NO 3 ) 2 , 2.5mM K 2 PO 4 , 70 μM H 3 BO 3 , 14 μM MnCl 2 , 1 μM ZnSO 4 , 0.5 μM CuSO 4 , 10 uM NaCl, 0.2 μM NaCl 2 MoO 4 , 40 μM FeEDTA, 0.8% agar, pH=5.7), the same method was cultivated for 3 days and then transferred to the iron-deficiency medium (40 μM FeEDTA was not added to the MS' medium, and 100 μM 5,6-diphenyl-3-( 2-pyridyl)-1,2,4-triazine-4,4'-disulfonic acid disodium salt) was incubated unde...

Embodiment 3

[0020] Example 3 The accumulation of EIN3 protein directly leads to vigorous growth of root hairs

[0021] Construct β-estrogen-induced clones expressing EIN3 and transform them into ein3 eil1 ebf1 ebf2 quadruple mutants. Since there is no EBF1 or EBF2 that degrades EIN3 and EIL1 proteins, β-estrogen-induced exogenous EIN3 protein can be expressed in plants Stable existence. Transgenic homozygous seeds were sown on MS medium, grown under light for 3 days, then transferred to MS medium supplemented with β-estrogen (10 μM), and cultured vertically under light for another 3 days to observe the phenotype. In the control experiment without adding estrogen induction, that is, without EIN3 and EIL1 proteins, it is difficult to observe root hairs; while adding β-estrogen to induce EIN3 gene expression and the translated protein product exists stably, you can see the roots of plant seedlings A lot of root hairs grow out. This shows that as long as the accumulation of EIN3 in the plan...

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Abstract

The invention provides an application of gene EIN3 and gene EIL1 in promoting the growth of plant root hair. The application is that the gene EIN3 and the gene EIL1 are expressed in the plant through a genetic engineering method. Through carrying out a molecular biological mechanism research on the gene EIN3 and the gene EIL1 in the mode plant Arabidopis thaliana, the nutrition absorption efficiency of real crops is improved by over expressing the gene EIN3 and the gene EIL1 in the plant, and the adverse stress resisting capacity of the crops is enhanced, so yield increase in the agriculture is achieved.

Description

technical field [0001] The invention belongs to the field of genetic engineering, and in particular relates to the application of genes EIN3 and EIL1 in promoting the development of plant root hairs. Background technique [0002] With the growth of population, how to increase crop yield to maintain human survival is an urgent problem to be solved. The first green revolution proposed in the middle of the last century used a large amount of pesticides and chemical fertilizers in agricultural production. Although the yield of crops was improved, it brought very serious problems. Excessive use of chemical fertilizers and pesticides not only causes a lot of waste, but also proves to be very harmful to human health. More importantly, excessive chemical fertilizers and pesticides pollute the surface and groundwater, and will decompose a large amount of greenhouse gases, which has caused worldwide Widespread concern. The second green revolution proposed in response to this problem...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29A01H5/00
Inventor 郭红卫朱自强冯莹
Owner PEKING UNIV
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