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Preparation method for total saponins of pulsatilla chinensis

A technology of Pulsatilla grandis and total saponins, which is applied in skin care preparations, medical preparations containing active ingredients, and pharmaceutical formulas, can solve the problem of high impurities in Pulsatilla grandis total saponins, and achieve low impurity content, low cost, and simple operation Effect

Inactive Publication Date: 2011-11-09
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing Pulsatilla saponins extraction technology mainly adopts methods such as alcohol extraction and macroporous resin column chromatography, such as the literature "Separation and Purification of Puls

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Grind 1kg of Pulsatilla root into coarse powder, use 10L of 70% (v / v) methanol to extract 3 times by microwave, combine the extracts, add diatomaceous earth with a total liquid volume of 5%, stir, filter, and concentrate the filtrate under reduced pressure at 55°C to 1 / 4 of the total volume to obtain the extraction concentrate; add an equal volume of water-saturated n-butanol for extraction, separate the n-butanol layer, recover n-butanol (which can be used for extraction again), and extract 4 times to obtain n-butanol Alcohol extract 350g; Dissolve the n-butanol extract in water and pass through a 500g polyamide resin column, wash with water until the sugar reaction is negative, then elute with 60% ethanol, collect the eluate, and pass the eluate again A neutral alumina column was used to collect the infusion solution, and the ethanol was recovered under reduced pressure to obtain an extract, which was vacuum-dried to obtain 101 g of total saponins of Pulsatilla pulsati...

Embodiment 2

[0030] Grind 2kg of Pulsatilla root into a coarse powder, use 30L of 80% (v / v) methanol microwave extraction twice, combine the extracts, add diatomaceous earth with a total liquid volume of 4%, stir, filter, and concentrate the filtrate at 55°C under reduced pressure to 1 / 4 of the total volume to obtain the extraction concentrate; add an equal volume of water-saturated n-butanol for extraction, separate the n-butanol layer, recover n-butanol (which can be used for extraction again), and extract 4 times to obtain n-butanol Alcohol extract 720g; Dissolve n-butanol extract in water and pass through a column equipped with 800g polyamide resin, wash with water until the sugar reaction is negative, then elute with 50% ethanol, collect the eluate, and pass the eluate through again A neutral alumina column was used to collect the infusion solution, and the ethanol was recovered under reduced pressure to obtain an extract, which was vacuum-dried to obtain 198 g of total saponins of Pul...

Embodiment 3

[0032] Grind 5 kg of Pulsatilla root into coarse powder, use 25 L of 75% (v / v) methanol for microwave extraction three times, combine the extracts, add diatomaceous earth with a total liquid volume of 8%, stir, filter, and concentrate the filtrate at 55°C under reduced pressure to 1 / 4 of the total volume to obtain the extraction concentrate; add an equal volume of water-saturated n-butanol for extraction, separate the n-butanol layer, recover n-butanol (which can be used for extraction again), and extract 4 times to obtain n-butanol Alcohol extract 1.7kg; after dissolving the n-butanol extract in water, pass through a 2500g polyamide resin column, wash with water until the sugar reaction is negative, then elute with 70% ethanol, collect the eluate, and then wash the eluate again Pass through the neutral alumina column, collect the injection liquid, recover ethanol under reduced pressure, obtain the extractum, and vacuum dry to obtain 454g of total saponins of Pulsatilla pulsati...

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Abstract

The invention discloses a preparation method for total saponins of pulsatilla chinensis, belonging to the technical field of natural medicine extracts. The preparation method comprises the following steps of: (1) crushing pulsatilla chinensis root into crude powder and microwave extracting with water or methanol to obtain an extract solution; (2) adding diatomite and stirring and filtering, decompressing and condensing filtrate to obtain the extracted concentrate of the pulsatilla chinensis; (3) extracting the concentrate with water-saturated normal butanol and recovering the normal butanol to obtain a normal butanol extract; (4) enabling the normal butanol extract to pass through a polyamide resin column and collecting an elution with deionized water and hydrous alcohol as eluent; and (5) enabling the eluent to pass through a neutral alumina column, decompressing and recovering ethanol to obtain extract, and vacuum drying to obtain the total saponins of pulsatilla chinensis. Compared with the prior art, the realized preparation method disclosed by the invention has the advantages of low cost, low impurity content, less pollution to the environment in production process and the like.

Description

Technical field: [0001] The invention belongs to the technical field of extraction of natural medicines, and relates to a preparation method of total saponins of Pulsatilla pulsatillae. Background technique: [0002] Pulsatilla, also known as old man's flower, Pulsatilla chinensis, is the dry root of the perennial herb Pulsatilla Chinensis (Bge.) Regel. It has the effects of clearing away heat and detoxifying, cooling blood and stopping dysentery. It is clinically used for amoebic dysentery, bacillary dysentery and vaginal Trichomoniasis and other diseases. Distributed in Northeast China, North China, Northwest China, East China, North Korea and Siberia, Russia. It was first recorded in "Shen Nong's Materia Medica". The active ingredients of Pulsatilla are mainly saponins, including Pulsatilla saponins A, B, C, D, saponin 1, saponin 2, betulinic acid, betulinic acid-3-O-α-L arabinoside, 3-oxo Betulinic acid, carotin, protopulsatilla, pulsatilla, pulsatilla, pulsatilla, et...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K8/97A61P35/00A61P31/04A61Q19/08A61K125/00A61K8/9789
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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