Preparation method for total saponins of pulsatilla chinensis

Abstract

The invention discloses a preparation method for total saponins of pulsatilla chinensis, belonging to the technical field of natural medicine extracts. The preparation method comprises the following steps of: (1) crushing pulsatilla chinensis root into crude powder and microwave extracting with water or methanol to obtain an extract solution; (2) adding diatomite and stirring and filtering, decompressing and condensing filtrate to obtain the extracted concentrate of the pulsatilla chinensis; (3) extracting the concentrate with water-saturated normal butanol and recovering the normal butanol to obtain a normal butanol extract; (4) enabling the normal butanol extract to pass through a polyamide resin column and collecting an elution with deionized water and hydrous alcohol as eluent; and (5) enabling the eluent to pass through a neutral alumina column, decompressing and recovering ethanol to obtain extract, and vacuum drying to obtain the total saponins of pulsatilla chinensis. Compared with the prior art, the realized preparation method disclosed by the invention has the advantages of low cost, low impurity content, less pollution to the environment in production process and the like.

Description

Technical field:

[0001] The invention belongs to the technical field of extraction of natural medicines, and relates to a preparation method of total saponins of Pulsatilla pulsatillae. Background technique:

[0002] Pulsatilla, also known as old man's flower, Pulsatilla chinensis, is the dry root of the perennial herb Pulsatilla Chinensis (Bge.) Regel. It has the effects of clearing away heat and detoxifying, cooling blood and stopping dysentery. It is clinically used for amoebic dysentery, bacillary dysentery and vaginal Trichomoniasis and other diseases. Distributed in Northeast China, North China, Northwest China, East China, North Korea and Siberia, Russia. It was first recorded in "Shen Nong's Materia Medica". The active ingredients of Pulsatilla are mainly saponins, including Pulsatilla saponins A, B, C, D, saponin 1, saponin 2, betulinic acid, betulinic acid-3-O-α-L arabinoside, 3-oxo Betulinic acid, carotin, protopulsatilla, pulsatilla, pulsatilla, pulsatilla, et...