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Method for screening degumming strain

A screening method and strain technology, applied in the field of microorganisms, can solve the problems of slow degumming speed, damage to the quality of hemp fiber, and low degumming rate of strains, and achieve the effects of improving efficiency, fast degumming speed, and high degumming rate

Inactive Publication Date: 2011-11-23
湖南润久科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can screen all kinds of strains that use hemp as nutrition, including strains that can decompose pectin, hemicellulose and cellulose, but the strains that decompose cellulose in hemp degumming will seriously damage hemp fibers quality, so not all the strains obtained by this method are suitable for biological degumming
In addition, the strains screened by these screening methods have low degumming rate and slow degumming speed, which restricts their application in biological degumming

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Utilize the screening method of the present invention to screen degummed bacterial strains

[0025] (1) Enrichment culture:

[0026] Add 1 g of the sample containing Pectin Bacillus into a 200 ml Erlenmeyer flask filled with 20 ml of sterile water, add sterile glass beads, and vibrate for 20 minutes in a shaker at 150 rpm. Take 10ml of the shaken solution, add it into 100ml enrichment medium, and place it in a constant temperature shaker at 35°C with a rotation speed of 170rpm for 48h. Enrichment medium: 5% ramie, 1% peptone, 0.3% beef extract, 0.5% NaCl, and the rest is water.

[0027] Preparation of enrichment medium: Cut 5g of raw ramie into small pieces about 1cm long, add water and heat on the electric stove, after boiling, switch to low heat and boil, when the ramie in the cup becomes soft and the solution turns brown, Pull out the ramie, then add 0.3g beef extract, 1g peptone, 0.5g NaCl, and make the solution volume up to 100ml. This ratio is refe...

Embodiment 2

[0037] Embodiment 2: Utilize the screening method of the present invention to screen the degummed bacterial strain

[0038] (1) Enrichment culture:

[0039] Add 1 g of samples containing Enterobacter aerogenes and Enterobacter agglomerates into a 200ml Erlenmeyer flask filled with 20ml sterile water, add sterile glass beads, and shake in a shaker at 150rpm for 10min. Take 10ml of the shaken solution, add it into 100ml enrichment medium, and place it in a constant temperature shaker at 35°C with a rotation speed of 170rpm for 24h. Enrichment medium: 3% ramie, 0.5% peptone, 0.1% beef extract, 0.3% NaCl, and the rest is water.

[0040] Preparation of enrichment medium: cut 3g of raw ramie into small pieces about 1cm long, add water and heat on the electric stove, after boiling, switch to low heat and boil, when the ramie in the cup becomes soft and the solution turns brown, Pull out the ramie, then add 0.1g beef extract, 0.5g peptone, 0.3g NaCl, and make the solution volume up ...

Embodiment 3

[0050] Embodiment 3: Utilize the screening method of the present invention to screen the degummed bacterial strain

[0051] (1) Enrichment culture:

[0052] Add 1 g of samples containing Bacillus subtilis, Bacillus polymyxa and Bacillus licheniformis into a 200ml Erlenmeyer flask filled with 20ml sterile water, add sterile glass beads, and vibrate in a shaker at 150rpm for 15min. Aspirate 10ml of the shaken solution, add it into 100ml enrichment medium, and place it in a constant temperature shaker at 35°C with a rotation speed of 170rpm for 36h. Enrichment medium: 10% ramie, 1.5% peptone, 0.5% beef extract, 1.0% NaCl, and the rest is water.

[0053] Preparation of the enrichment medium: cut 10g of raw ramie into small pieces about 1cm long, add water and heat it on the electric stove, after boiling, switch to low heat and boil, when the ramie in the cup becomes soft and the solution turns brown, Pull out the ramie, then add 0.5g beef extract, 1.5g peptone, and 1.0g NaCl, an...

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Abstract

The invention relates to the field of microorganisms, and particularly discloses a method for screening a degumming strain. The screening method comprises the following steps of: inoculating a sample containing pectinatus, bacillus or enterobacteria into an enriched medium for culturing; coating the cultured culture onto a primary screening culture medium for culturing; purifying a colony which produces a hydrolyzed circle on the primary screening culture medium; and inoculating the purified strain onto a secondary screening culture medium, culturing for 15-24 hours and testing the degumming rate of the strain, wherein a strain with the degumming rate over 80 percent is a screened target strain. In the method for screening the degumming strain, ramie juice is taken as a unique organic nutritional ingredient for screening, a strain for decomposing the gum ingredient of ramie can be obtained quickly and repeatedly, and the cellulose fiber ingredient of ramie is not damaged; and moreover, the strain has high degumming rate, high degumming speed and industrial application.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for screening degummed bacterial strains. Background technique [0002] Ramie, also known as white-leaf ramie, is a perennial herbaceous plant. It is a unique crop in China mainly used for textiles. It has long single fibers, high strength, fast moisture absorption and dissipation, and good thermal conductivity. After degumming, it is white and mercerized. Pure spinning can also be blended with cotton, silk, wool, chemical fiber, etc. It is an important textile fiber crop. [0003] Ramie contains a lot of impurities, mainly polysaccharide colloidal substances. Before spinning, most of the colloids in the bast are required to be removed, and the single fibers of ramie are separated from each other. This process is called degumming. The traditional degumming process adopts the chemical degumming method, that is, the degumming method of caustic soda cooking. This method has s...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 杨政曾晓希仓道平
Owner 湖南润久科技有限公司
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