Phytoene dehydrogenase gene and application thereof

Abstract

The invention relates to a phytoene dehydrogenase gene, in particular to a phytoene dehydrogenase gene for efficiently synthesizing lycopene and application thereof, belonging to the field of gene engineering. The nucleotide sequence of the phytoene dehydrogenase gene is shown by SEQ ID No.1. The amino acid sequence of the phytoene dehydrogenase coded with the gene is shown by SEQ ID No.2. The phytoene dehydrogenase gene provided by the invention can be used for efficiently producing lycopene. The examination results in that the yield of lycopene in a fermentation liquor is 0.269 mg / L and thecontent of lycopene in the dry weight of bacteria is 0.256 mg / g and accounts for 73.5% the total content of carotenoid; the phytoene dehydrogenase recombinase catalysate of rhodobacter azotoformans uses lycopene as the main pigment component and provides a new enzyme source for producing lycopene.

Description

field of invention

[0001] The invention relates to a phytoene dehydrogenase gene, in particular to a phytoene dehydrogenase gene for efficiently synthesizing lycopene and its application, belonging to the technical field of genetic engineering. technical background

[0002] Lycopene is currently the strongest antioxidant found in nature. It can efficiently quench singlet oxygen and scavenge free radicals. Its singlet oxygen quenching rate constant is 100 times that of vitamin E. Therefore, lycopene has important physiological functions , such as anti-cancer, anti-aging, improving immunity, etc.

[0003] At present, commercial lycopene is mainly obtained from tomatoes. This method requires a large amount of tomatoes to ensure the continuity and low cost of industrial production. Moreover, tomatoes are inconvenient to transport, and their acquisition is greatly affected by climatic conditions. Due to the deterioration of the environment and the improvement of people's health ...

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