Method for identifying drought resistance of tobacco at seedling stage
A technology of drought resistance and tobacco, applied in the field of identification of plant drought resistance, can solve the problems of immature technical system, no method patent, time-consuming and labor-consuming, etc., and achieve the effect of drought resistance evaluation
Inactive Publication Date: 2015-07-29
GUIZHOU TOBACCO SCI INST
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Problems solved by technology
Most of the reports on tobacco drought resistance research use potted drought treatment, which has a long cycle, time-consuming and labor-intensive, and the technical system is immature
Tobacco drought resistance identification technology has been immature, and the main reason why it is difficult to apply is that there has been no suitable drought-resistant control variety in tobacco. Without comparison, it is difficult to evaluate and identify the drought resistance of general varieties; and there is no relevant data on the degree of drought suitable for tobacco drought resistance identification.
[0003] The technical solutions related to crop drought resistance in the Chinese patent database include No. 200410061011.7 "Improving Plant Drought Resistance Using Rice Drought-Inducible Gene Promoter LEAP", No. 200710178721.1 "A Method for Identification of Drought Resistance", No. 200810036342.3 "Using Mouse Nitric Oxide Synthesis Enzyme gene improves drought resistance of plants", No. 200910272694.3 "Application of MAPKKK family gene DSM1 gene in controlling drought resistance of rice", etc., but so far, no method patent for identifying drought resistance of tobacco has been found
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[0017] Example Study on the Drought Resistance Identification of 12 Tobacco Varieties
[0018] (1) Take 12 tobacco varieties plus 2 control varieties, adopt floating seedling cultivation, seedling cultivation management is carried out as usual, 70 plants (half a floating tray) for each variety, set 3 repetitions; prepare for drought when the seedling age is about 60 days Processing; (2) all seedling-raising floating trays are taken out from the seedling-raising pool and put on a shelf (cut off all water sources), so that the environmental conditions such as light and ventilation of all varieties are kept consistent. The temperature is controlled at 30 degrees in the greenhouse, and not lower than 20 degrees at night (that is, the first level of drought treatment / moderate drought). (3) To observe the drought condition of the tobacco seedlings, it was found that the medium-resistant control (Majiang Dahonghua) wilted and could not recover naturally on the 4th day. All species w...
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The invention discloses a method for identifying the drought resistance of tobacco at the seedling stage, which is carried out in a greenhouse. The method comprises the following steps: (1) variety selection; (2) seedling cultivation; (3) drought treatment; (4) restoration of water supply; ( 5) Re-drought treatment; (6) Restoring water supply again; (7) Identification, to determine the drought resistance of tobacco varieties. The method for identifying the drought resistance of tobacco at the seedling stage of the present invention adopts the drought treatment on the floating tray for seedling cultivation to realize the rapid identification of the drought resistance of tobacco varieties at the seedling stage, and establishes a systematic drought-resistant appraisal technology system for tobacco seedling stage for the first time; through the two-stage drought method, it can Clearly distinguish the drought resistance characteristics of different tobacco varieties, and use the screened medium and high resistance control varieties, so that the drought resistance evaluation of tobacco has a relative standard. This technical system can realize the drought resistance evaluation of a large number of tobacco varieties and germplasm resources.
Description
technical field [0001] The invention relates to a method for identifying drought resistance of plants, in particular to a method for identifying drought resistance of tobacco. Background technique [0002] Drought resistance identification technology has been studied more in other crops such as wheat and corn. There are also reports of identification at the seedling stage in corn, etc. Due to different crops, identification methods vary greatly. Most of the reports on tobacco drought resistance research use potted drought treatment, which has a long cycle, time-consuming and labor-intensive, and the technical system is immature. Tobacco drought resistance identification technology has been immature, and the main reason why it is difficult to apply is that there has been no suitable drought-resistant control variety in tobacco. Without comparison, it is difficult to evaluate and identify the drought resistance of general varieties; and there is no relevant data on the degre...
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IPC IPC(8): A01G1/00A01G7/06
Inventor 王仁刚任学良李莉张吉顺罗正友
Owner GUIZHOU TOBACCO SCI INST