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Polypeptide vaccine and preparation method thereof

A polypeptide vaccine and polypeptide solution technology, which is applied in the direction of pharmaceutical formula, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve problems such as inflammation or allergic reactions, and affect the specificity of antigenic peptides, achieving significant Effects of immune activity, guaranteed quality, and low production cost

Inactive Publication Date: 2013-03-13
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many technical problems in this type of antigenic peptide-carrier protein compound vaccine. For example, there is carrier protein in the antigenic peptide-carrier protein compound vaccine, and the carrier protein is usually a heterogeneous protein, which easily causes inflammation or allergic reaction when inoculated. Can affect the specificity of the antigenic peptide
Due to these side effects, the antigenic peptide-carrier protein compound vaccine has not been widely applied in the market

Method used

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  • Polypeptide vaccine and preparation method thereof
  • Polypeptide vaccine and preparation method thereof
  • Polypeptide vaccine and preparation method thereof

Examples

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preparation example Construction

[0032] The preparation method of the above-mentioned polypeptide vaccine comprises the following steps:

[0033] 1) Dissolving the immunoactive peptide to obtain a peptide solution for later use;

[0034] 2) Dissolving the non-protein carrier to obtain a non-protein carrier solution, set aside;

[0035] 3) Add coupling agent to the polypeptide solution or non-protein carrier solution, and mix well;

[0036] 4) Mix the non-protein carrier solution and the polypeptide solution evenly, stir and react, so that the polypeptide is coupled to the non-protein carrier;

[0037] 5) After the reaction is complete, remove the coupling agent that did not participate in the reaction, and purify the coupled polypeptide;

[0038] 6) Adding a medically acceptable adjuvant to the coupled polypeptide to obtain a polypeptide vaccine.

[0039] Preferably, the mass mixing ratio of the polypeptide to the non-protein carrier is 1-100:1.

[0040] Preferably, the mass mixing ratio of the non-protei...

Embodiment 1

[0044] Polypeptide vaccine for porcine blue ear disease and preparation method thereof

[0045] 1. Peptide screening and synthesis

[0046] 1) Screening of peptides

[0047] Predict the epitope of the structural glycoprotein of porcine blue ear virus (PRRSV) by computer analysis software, determine the amino acid sequence of the natural antigen, and then search for the epitopes targeted by the peptide, and select the primary polypeptide sequence.

[0048] The reaction between different polypeptides and porcine PRRS virus antiserum was detected by enzyme-linked method (ELISA), and finally the B cell epitope polypeptides P1~P4 with good reactogenicity and immunogenicity were selected, and their sequences are as follows:

[0049] P1: SHIQLIYNLNC (SEQ ID NO: 1)

[0050] P2: SHIQLIYNLTLCEPL (SEQ ID NO: 2)

[0051] P3: VEKGFKVVFGNVSGIVA (SEQ ID NO: 3)

[0052] P4: RKVYVDIKHQFICADH (SEQ ID NO: 4)

[0053] Using polypeptide to stimulate porcine T cells (IFNγ / ELISPOT) method, ...

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Abstract

The invention discloses a polypeptide vaccine. An active component of the polypeptide vaccine is a coupled polypeptide which is obtained by coupling an immunoactive polypeptide to a non-protein carrier. The polypeptide vaccine of the present invention not only has substantial immunocompetence and stability, but also allows toxic and side effects of inflammation or anaphylactic reactions caused bytraditional polypeptide-carrier protein vaccines to be avoided, so the security and the specificity of the polypeptide vaccine are substantially improved. The invention also provides a preparation method of the polypeptide vaccine, and the preparation method has the advantages of simplicity and easy implement, low production cost, quality guarantee, and easy scale production

Description

technical field [0001] The invention relates to a polypeptide vaccine and a preparation method thereof. Background technique [0002] Peptide vaccines are vaccines prepared by chemical synthesis techniques according to the amino acid sequence of a known or predicted epitope in the pathogen antigen gene. As a part of the complete virus, the polypeptide antigen does not have the risk of infectious diseases, and can be synthesized and produced in large quantities, which has become one of the important ways of vaccine development. [0003] However, because the molecular weight of the antigenic polypeptide is too small, it is not easy to generate an immune response, and it is often necessary to be connected to a carrier protein to enhance the immune effect. Mainly use glutaraldehyde and other linking molecular methods to connect effective antigenic peptides to carrier proteins keyhole limpet hemocyanin (KLH), human serum albumin (HSA), ovalbumin (OVA), bovine serum albumin (BSA)...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K47/48
Inventor 李其昌拓荒王贵平林旭埜李嘉爱穆光慧陈善真
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST