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Filamentous fungus for efficiently degrading lignocellulose in forest litter

A technology of lignocellulose and filamentous fungi, applied in the direction of fungi, microorganism-based methods, microorganisms, etc., can solve the problems of low forestry production level, achieve the goal of improving forest nutritional status, promoting material circulation, and improving forestry production level Effect

Inactive Publication Date: 2012-12-19
HEILONGJIANG UNIV
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Problems solved by technology

[0004] The present invention provides a filamentous fungus that efficiently degrades lignocellulose in forest litter to solve the problem that current forestry production completely relies on microorganisms existing in the natural environment and the level of forestry production is low.

Method used

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  • Filamentous fungus for efficiently degrading lignocellulose in forest litter
  • Filamentous fungus for efficiently degrading lignocellulose in forest litter

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specific Embodiment approach 1

[0012] Specific embodiment 1: In this embodiment, the filamentous fungus that efficiently degrades lignocellulose in forest litter is Penicillium sp. HDSF3, which is deposited in the General Microbiology Center of the China Microbial Species Collection Management Committee, and the preservation number is CGMCC No. 5029.

[0013] In this embodiment, Penicillium HDSF3 was selected from the Korean pine forest of Fenglin National Nature Reserve in Heilongjiang Province. It was morphologically identified as Penicillium sp.

[0014] In this embodiment, litters are collected at multiple points in the red pine forest in the Fenglin National Nature Reserve of Heilongjiang Province according to the zigzag route. Using the section sampling method, sampling from top to bottom according to the color, structure, decomposition degree, etc. of the litter, put them in polyethylene bags and number them, and store them at 4℃; then, the fungus was separated by the dilution plate method. The filament...

specific Embodiment approach 2

[0025] Embodiment 2: In this embodiment, Penicillium HDSF3 in the first embodiment is fermented and cultured in a fermentor (the culture medium and culture conditions of the fermentation culture in this embodiment are the same as those in the pure fermentation of Penicillium HDSF3 in the first embodiment), Make a concentration of 10 3 CFU / mL Penicillium HDSF3 bacterial suspension, and then diluted with water according to the ratio of 1:10 to make Penicillium HDSF3 spray liquid, sprayed on the sterilized Korean pine needles (60℃ ethylene oxide gas) according to the ratio of 1mL / g Sterilize in medium for 3h), and then put the red pine needles inoculated with Penicillium HDSF3 in a decomposing bag with a mesh diameter of 0.5 mm. Potted 1-year-old Korean pine seedlings were used as test materials, and turf soil, vermiculite and sand (volume ratio 5:3:2) were used as culture substrates, and the decomposition bag was spread flat on the culture substrate. The experiment was divided in...

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Abstract

The invention discloses a filamentous fungus for efficiently degrading lignocellulose in forest litter and relates to a microorganism for degrading the lignocellulose in the forest litter. The invention solves the problems that microorganisms existing in the natural environment are completely depended in the current forest production and the level of the forest production is lower. The filamentous fungus for efficiently degrading the lignocellulose in the forest litter is Penicillium sp. HDSF3 and is collected in China General Microbiological Culture Collection Center, and the collection number is CGMCC No. 5029.

Description

Technical field [0001] The invention relates to a microorganism that degrades lignocellulose in forest litter. Background technique [0002] The decomposition of litter is an important part of the chemical cycle of forest ecosystems, and it affects the productivity of forest ecosystems. The decomposition of forest litter can increase the nutrient content of the surface soil and improve the chemical composition of the soil, which is beneficial to the growth of plants. [0003] At present, the decomposition of forest litter still depends on the microorganisms in the natural environment. Because the types and quantity of microorganisms in the natural environment are affected by many factors, there is a large fluctuation, so the decomposition level of forest litter varies greatly. . In order to improve forestry production levels, improve forest nutrition, and promote material circulation, there is an urgent need for a microorganism that can efficiently degrade forest litter. Summary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/80
Inventor 宋福强孟剑侠
Owner HEILONGJIANG UNIV
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