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Jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and preparation method thereof

A kind of seed soaking agent, the technology of jatropha, applied in the field of jatropha germination-promoting and stress-resistant seed soaking agent and its preparation

Inactive Publication Date: 2012-01-25
YUNNAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The existing seed soaking agent technology is mainly used in crops rice, wheat and sugar beet, etc., but there are fewer application reports in woody plants. growth and development has not been reported

Method used

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  • Jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and preparation method thereof
  • Jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and preparation method thereof
  • Jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The raw material formula of jatropha seed soaking agent is: gibberellin 10mg / L; calcium chloride 5mmol / L; betaine 15mmol / L; trace elements: KI 0.78mg / L, MnSO 4 18.6mg / L, ZnSO 4 7.9mg / L, Na 2 MoO 4 0.12mg / L, CuSO 4 0.023mg / L, CoCl 2 0.023mg / L, the rest is triple distilled water.

[0021] Preparation method: ① Weigh calcium chloride and dissolve it in triple distilled water; ② Weigh betaine and dissolve it in triple distilled water; ③ Add trace elements (make mother liquor); ④ Dissolve gibberellin in a small amount of absolute ethanol first, Mix with the previous part; ⑤Finally, dilute to the required volume with triple distilled water.

[0022] Effect:

[0023] Under the treatment of this seed soaking agent, the germination of Jatropha seeds was improved no matter under normal conditions, cold (18 ℃) stress, or drought (5% polyethylene glycol 6000) stress, such as figure 1 As shown, under normal germination, cold or drought treatment conditions, its germinat...

Embodiment 2

[0025] The raw material formula of jatropha seed soaking agent is: gibberellin 5mg / L; calcium chloride 10mmol / L; betaine 10mmol / L; trace elements: KI 0.83mg / L, MnSO 4 22.3mg / L, ZnSO 4 8.6mg / L, Na 2 MoO 4 0.25mg / L, CuSO 4 0.025mg / L, CoCl 2 0.025mg / L, the rest is triple distilled water.

[0026] Preparation method: ① Weigh calcium chloride and dissolve it in triple distilled water; ② Weigh betaine and dissolve it in triple distilled water; ③ Add trace elements (make mother liquor); ④ Dissolve gibberellin in a small amount of absolute ethanol first, Mix with the previous part; ⑤Finally, dilute to the required volume with triple distilled water.

[0027] Effect:

[0028] Under the treatment of Jatropha Jatropha seedlings under cold (2℃, treatment for 5 d) stress, the electrolyte leakage rate in roots and leaves increased and the accumulation of MDA content was alleviated, and the electrolytes in roots and leaves The leakage rate decreased by 31.5% and 30.1% respectivel...

Embodiment 3

[0030] The raw material formula of jatropha seed soaking agent is: gibberellin 10mg / L; calcium chloride 20mmol / L; betaine 20mmol / L; trace elements: KI 0.85mg / L, MnSO 4 25.3mg / L, ZnSO 4 8.8mg / L, Na 2 MoO 4 0.28mg / L, CuSO 4 0.027mg / L, CoCl 2 0.027mg / L, the rest is triple distilled water (distilled three times).

[0031] Preparation method: ① Weigh calcium chloride and dissolve it in triple distilled water; ② Weigh betaine and dissolve it in triple distilled water; ③ Add trace elements (make mother liquor); ④ Dissolve gibberellin in a small amount of absolute ethanol first, Mix with the previous part; ⑤Finally, dilute to the required volume with triple distilled water.

[0032] Effect:

[0033]Under the treatment of the seed soaking agent, the simulated drought stress of 25% polyethylene glycol 6000 (PEG6000) was relieved for 5 days, and the electrolyte leakage rate in the roots and leaves of Jatropha Jatropha seedlings increased and the content of malondialdehyde (MDA...

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Abstract

The invention relates to a jatropha curcas L. germination-accelerating stress tolerance seed soaking agent and a preparation method thereof. In a formula, each liter of jatropha curcas L. germination-accelerating stress tolerance seed soaking agent comprises the following raw materials: 5 to 15 mg of gibberellin, 5 to 20 mmol of calcium chloride, 10 to 20 mmol of betaine, and 0.78 to 0.85 mg of KI, 18.6 to 25.3 mg of MnSO4, 7.9 to 8.8 mg of ZnSO4, 0.12 to 0.28 mg of Na2MoO4, 0.023 to 0.027 mg of CuSO4 and 0.023 to 0.027 mg of CoCl2 which serve as trace elements and the balance of triple-distilled water. By the seed soaking agent, the germination rate of jatropha curcas L. seeds which are normally germinated and are processed at a low temperature under the drought condition can be improved, and the resistance capacity of jatropha curcas L. seedlings on low temperature stress and drought stress can be improved.

Description

technical field [0001] The invention relates to the technical field of plant cultivation, in particular to a jatropha seed soaking agent for promoting germination and stress resistance and a preparation method thereof. Background technique [0002] Jatropha ( Jatropha curcas L.) is a deciduous shrub or small tree of the family Euphorbiaceae (Euphorbiaceae) Jatropha Linn (Jatropha Linn), also known as Jatropha curcas, tung tree, etc. Dry and hot valley areas with little rainfall. Jatropha seeds have high oil content and can be used as raw materials for biodiesel, bioenergy development, biomedicine and pesticides, organic compound fertilizers, industrial raw materials, etc. plant. [0003] Jatropha Jatropha is native to tropical and subtropical regions and has weak cold resistance. The suitable annual average temperature for growth is 19.3-27.2 ℃, and the lowest monthly average temperature is higher than 10.5 ℃. Low temperature is the main limiting factor affecting the su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/20A01N59/16A01N59/12A01N59/08A01N43/12A01N37/44A01P21/00
Inventor 龚明代勋许锁链李忠光
Owner YUNNAN NORMAL UNIV
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