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Preparation method for momordin

A technology of Kochia saponins and Kochia chinensis, which is applied in the field of medicine, can solve the problems of organic solvent toxicity residue and high cost, and achieve the effect of short extraction time, low cost and large processing capacity

Inactive Publication Date: 2012-03-14
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, most of the purification methods of Kochia saponins use alcohol extraction and n-butanol extraction, which has the disadvantages of high cost and toxic residual organic solvents.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take 1kg of crushed skin into 50 destinations and input it from one end of the extraction tube, add 8L of pH 10 sodium bicarbonate aqueous solution from the other end of the extraction tube as the extraction solvent, start the spiral stirring and ultrasonic generator, the ultrasonic power is 30kHz, after 0.5 hours of extraction, the liquid residue is separated. The separated Kochia scoparia residue is subjected to a second ultrasonic extraction. The two extracts are combined and passed through a D61 macroporous cationic resin column. The effluent is then passed through AB-8 macroporous adsorption Resin column, adsorption 3 times, first eluted with 20% ethanol, and then eluted with 80% ethanol on the resin column, collect the eluate, concentrate the eluate to a relative density of 1.1-1.2, add 1% of medicinal activated carbon , Heated to reflux for 0.5 hours, filtered while hot, the decolorizing solution was decompressed to recover ethanol to obtain an extract, washed with...

Embodiment 2

[0020] Take 8kg crushed into 50 destination skins and input it from one end of the extraction tube, add 48L of pH 9 sodium carbonate aqueous solution from the other end of the extraction tube as the extraction solvent, start the spiral stirring and ultrasonic generator, the ultrasonic power is 40kHz After 2 hours of extraction, the liquid residue is separated, the separated Kochia scoparia residue is subjected to a second ultrasonic extraction, and the two extracts are combined and passed through a D61 macroporous cationic resin column. The effluent is then passed through a HZ806 macroporous adsorption resin column. Adsorb 3 times, first eluting with 20% ethanol, then eluting the resin column with 40% ethanol, collect the eluate, concentrate the eluate to a relative density of 1.1-1.2, add 0.8% medicinal activated carbon, and heat to reflux After 1 hour, filter while hot, and recover the ethanol from the decolorizing solution under reduced pressure to obtain the extract. After w...

Embodiment 3

[0022] Take 18kg of crushed skin into 50 destinations and input it from one end of the extraction tube, add 90L of potassium hydroxide aqueous solution with pH 11 as the extraction solvent from the feeding device at the other end of the extraction tube, start the spiral stirring and ultrasonic generator, the ultrasonic power is 28kHz, after 1.5 hours of extraction, the liquid residue is separated. The separated Kochia scoparia residue is subjected to a second ultrasonic extraction. The two extracts are combined and passed through a D61 macroporous cationic resin column. The effluent is then passed through D101-I macroporous adsorption Resin column, adsorb twice, first eluting with 20% ethanol, then eluting the resin column with 70% ethanol, collect the eluate, concentrate the eluate to a relative density of 1.1-1.2, add 0.6% of medicinal activated carbon , Heated to reflux for 0.5 hours, filtered while hot, the decolorizing liquid was decompressed to recover ethanol to obtain an...

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PUM

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Abstract

The invention relates to the technical field of medicines, and specifically to a preparation method for momordin. According to the invention, continuous countercurrent ultrasonic extraction is employed to prepare momordin; the medicinal material of broom cypress fruit is crushed to 20 to 100 meshes, the cavitation effect of ultrasonic wave is utilized for extraction so as to prepare a liquid extract of momordin, the liquid extract passes through an ion exchange column and a macroporous adsorption resin column, medicinal active carbon is added into the liquid extract for decolouring, decolored liquid is condensed to obtain an extract, and the extract is rinsed with acetone and is subjected to pressure spray drying to obtain high purity momordin. The preparation method for momordin provided in the invention has the advantages of a high extraction rate, great treating capacity, clean and health production process, energy conservation, continuous operation and capability of producing high purity momordin.

Description

Technical field: [0001] The invention belongs to the technical field of medicine and relates to a method for preparing Kochia saponins from Kochia scoparia. Background technique: [0002] Kochia scoparia is the mature fruit of Kochia scoparia (L.) Schard. Chenopodium scoparium L., also known as scoparium scoparium. It has the functions of clearing heat and dampness, dispelling wind and relieving itching, diuresis and leaching. [0003] Kochia scoparia fruits mainly contain triterpenoids and their glycosides, alkaloids, flavonoids and fatty oils. Triterpene saponins include oleanolic acid, cochinchinensis Ic (also known as cochinchinensis saponins Ic), cochinchinensis Protosides II c, etc., among which Kochia saponins Ic (momordin Ic) is the main effective ingredient for its anti-itch, anti-inflammatory and anti-allergic effects. [0004] Pharmacological experiments show that Kochia saponins have antibacterial effects. The 70% alcohol extract can inhibit inflammation and type I, III,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/70A61P17/04A61P29/00A61P37/08A61P31/04A61K131/00
Inventor 李法庆郭琴刘东锋
Owner 苏州宝泽堂医药科技有限公司
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