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Corynebacterium pekinense and application thereof

A technology of Corynebacterium beijingensis and seeds, applied in the field of microorganisms, can solve the problem of low L-lysine yield and so on

Active Publication Date: 2012-03-21
新疆梅花氨基酸有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of L-lysine is inseparable from the type of microorganisms used. Corynebacterium Beijing is one of the commonly used strains in L-lysine fermentation, but its L-lysine production is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Screening of Corynebacterium Beijing MHZ-0910 according to the present invention

[0021] Inoculate the starting strain - wild-type Corynebacterium Beijing CICC 21721 in LB liquid medium at 30°C for 16 hours to obtain a bacterial suspension in the logarithmic growth phase, and then spread the bacterial suspension on an LB solid plate, and apply an appropriate amount of Put the N-methyl-N'nitro-N-nitrosoguanidine solid particles in the center of the LB solid plate, place it in a 30°C incubator for 16 hours, and then scrape off the N-methyl-N'nitro-N - There is a weakly growing bacterial lawn around the nitrosoguanidine particles. Suspend in an appropriate amount of sterile normal saline, spread the normal saline with bacterial lawn suspended on an LB solid plate containing 0.5 mg / L, and continue to cultivate at 30°C for 2 According to the characteristics of colony shape and color, a single colony was picked and named MHZ-0910.

[0022] The formula of LB liqui...

Embodiment 2

[0025] Embodiment 2: L-lysine production method and L-lysine fermentation comparison test of the present invention

[0026] 1. Fermentation method

[0027] Corynebacterium Beijing MHZ-0910 described in the present invention was inoculated in the seed medium described below to multiply, and cultured with shaking at 30°C for 16 hours, and the resulting culture was inoculated into the fermentation medium described below, and the culture was mixed with The volume ratio of the fermentation medium is 1:10 (those skilled in the art can adjust appropriately according to the amount of the fermentation medium in actual production, this ratio is only a preferred ratio), shake culture at 30°C until L-lysine is no longer produced (Generally about 72 hours). During shaking culture in the fermentation medium, maintain the pH value in the fermentation medium between 6.5-7.4.

[0028] Seed Medium:

[0029] 20g / L glucose, 5g / L ammonium sulfate, 1g / L potassium dihydrogen phosphate, 30g / L corn...

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Abstract

The invention relates to the field of microorganisms and discloses a corynebacterium pekinense, wherein the collection number thereof is CGMCC No.5361. The corynebacterium pekinense with the collection number of CGMCC No.5361 takes a wild corynebacterium pekinense with the collection number of CICC No.21721 as a parent strain, and is prepared by performing chemical mutagenesis and screening through a culture medium containing neomycin; and the capability of generating L-lysine through fermentation is obviously higher than that of the wild corynebacterium pekinense, and the yield of the L-lysine is increased by 14.7 percent, so that the corynebacterium pekinense with the collection number of CGMCC No.5361 is widely applied to the fermentation of the L-lysine.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a Corynebacterium beijingensis and its application. Background technique [0002] L-Lysine, scientifically named 2,6-diaminocaproic acid, is a basic amino acid among the 20 common amino acids that make up proteins, and is one of the essential amino acids for the human body. [0003] Protein is the main component of human cells. After entering the human body, protein in food is first decomposed into amino acids after digestion, and then the human body uses these amino acids to synthesize new human proteins, such as immune antibodies, digestive enzymes, plasma proteins, growth hormones, etc. It is a synthetic human protein. Among the various amino acids in the synthesis of proteins, L-lysine is the most important one. Without it, other amino acids will be restricted or not utilized. Scientists call it the first essential amino acid for the human body. In recent years, scientists hav...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P13/08C12R1/15
Inventor 周希贵高云飞李岩胡征宇何君
Owner 新疆梅花氨基酸有限责任公司
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