Use of cathepsin L inhibitor in preparation of radiotherapeutic sensitization drug

A cathepsin and inhibitor technology, applied in the field of radiotherapy sensitizing drugs, can solve the problem of radiosensitization effect of cathepsin L inhibitor, etc., and achieve the effect of overcoming the radioresistance of tumor cells

Inactive Publication Date: 2012-04-25
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This technology allows researchers to study how cathepsins work together during cancer treatment without causing harm or killing normal tissues around them. It also shows potential benefits over existing treatments such as chemotherapies (chemo).

Problems solved by technology

This patents discuss how cancer develops from brain stem cells due to their genetic instability or abnormal functioning during normal development processes like embryogenesis. These issues include increased risk of developing resistances towards these agents, leading to reduced efficacy against them.

Method used

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  • Use of cathepsin L inhibitor in preparation of radiotherapeutic sensitization drug
  • Use of cathepsin L inhibitor in preparation of radiotherapeutic sensitization drug
  • Use of cathepsin L inhibitor in preparation of radiotherapeutic sensitization drug

Examples

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Embodiment 1

[0043] (1) Establish an in vitro culture system of human glioma stem cells and an orthotopic xenograft model in nude mice. The orthotopic tumor transplantation model in nude mice is a routine technique in the laboratory. Glioma stem cells in the logarithmic growth phase are taken for use. Take a 5-week-old nude mouse for anesthesia, cut the skin of the head, and use a bone drill to place it on the skull of the nude mouse Drill the hole, fix the nude mouse with a mouse stereotaxic instrument, and slowly inject the cells into the nude mouse brain with a micro-injection pump, the cell volume is 1×10 6 , slowly pull out the needle, and suture the wound.

[0044] (2) Immunofluorescence and Western blot were used to observe the changes in the expression of Cathepsin L in glioma stem cells after X-ray treatment:

[0045] Immunofluorescence method treatment steps: take logarithmic growth phase glioma stem cells, inoculate them in 6-well cell culture plates, and give 6Gy X-ray treatme...

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Abstract

The invention belongs to the field of radiotherapeutic sensitization drugs and particularly discloses novel use of a cathepsin L inhibitor in the preparation of a radiotherapeutic sensitization drug. The invention firstly proves that the activity of cathepsin L is related to the radioresistant mechanism of glioma stem cells and is an important regulating factor in the mechanism for restoring and periodically regulating the damage to the DNA of the glioma stem cells caused by radiation, and the cathepsin L inhibitor can effectively overcome the phenomenon of radioresistance of tumor cells, so that an experimental basis is provided for proving that the cathepsin L is a new acting target position in the radiotherapy of glioma. The invention simultaneously provides the novel use of the cathepsin L inhibitor in the preparation of the radiotherapeutic sensitization drug, wherein the cathepsin L inhibitor is selected from Z-phenylalanine-tyrosine tert-butyl-nitrous ketone or cathepsin siRNA.

Description

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Claims

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Application Information

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Owner SUZHOU UNIV
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