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Method for endowing collagen with lasting antibacterial function by using outer wall quaternized cucurbituril

A technology of quaternization and cucurbituril is applied in the preparation methods of peptides, chemical instruments and methods, botanical equipment and methods, etc., and can solve the problems of easy migration, destruction of triple helix structure, and decreased antibacterial and antiseptic function of collagen, etc. achieve the effect of retaining biological activity

Active Publication Date: 2022-01-25
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Directly adding antibacterial agents can effectively prevent the growth of collagen bacteria, but there is no binding force between the externally added antibacterial agents and collagen, which is prone to migration, resulting in a decrease in the antibacterial and antiseptic function of collagen
Covalently grafting antibacterial agents onto the surface of collagen can solve the long-term antibacterial problem, but most of the chemical reactions are too violent, which may destroy the triple helix structure that determines the biological activity of collagen, reducing its practical application value

Method used

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  • Method for endowing collagen with lasting antibacterial function by using outer wall quaternized cucurbituril
  • Method for endowing collagen with lasting antibacterial function by using outer wall quaternized cucurbituril

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 1. Disperse 1 part of cucurbit [7] urea and 3 parts of potassium persulfate in 30 parts of distilled water, react for 6 hours under the protection of nitrogen at 60°C, cool and filter after the reaction, dry the filtrate in vacuum, and then dissolve the obtained solid in 1 part of dimethyl sulfoxide, then filtered, 50 parts of acetone was added to the filtrate to obtain a precipitate, and finally the precipitate was washed with acetone and dried to a constant weight to obtain the first-step synthetic product;

[0023] 2. Dissolve 1 part of the first step synthesis product in 10 parts of dimethyl sulfoxide, then add 2 parts of sodium hydride, react at room temperature for 1 hour, then add 1 part of chlorinated choline chloride to the above solution, and stir at room temperature After 12 hours, after the reaction was over, the precipitate was collected and vacuum-dried to constant weight to obtain the target product;

[0024] 3. Dissolve 3 parts of the above target produc...

Embodiment 2

[0027] 1. Disperse 2 parts of cucurbit [7] urea and 3 parts of potassium persulfate in 60 parts of distilled water, react for 8 hours under the protection of nitrogen at 80°C, cool and filter after the reaction, dry the filtrate in vacuum, and dissolve the obtained solid in 2 parts of dimethyl sulfoxide, then filtered, 75 parts of acetone was added to the filtrate to obtain a precipitate, and finally the precipitate was washed with acetone and dried to a constant weight to obtain the first-step synthetic product;

[0028] 2. Dissolve 1 part of the first step synthesis product in 10 parts of dimethyl sulfoxide, then add 2 parts of sodium hydride, react at room temperature for 1 hour, then add 1 part of bromobromocholine to the above solution, and stir at room temperature After 12 hours, after the reaction was over, the precipitate was collected and vacuum-dried to constant weight to obtain the target product;

[0029] 3. Dissolve 3 parts of the above target product in 40 parts ...

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Abstract

The invention discloses a method for endowing collagen with a lasting antibacterial function by using supramolecular chemistry. The method comprises the following steps of firstly preparing cucurbit [7] uril of which the outer wall contains a plurality of short-chain quaternary ammonium groups, then introducing quaternary ammonium groups into the surface of collagen by utilizing the supramolecular inclusion effect of the cucurbit [7] uril on phenylalanine and tyrosine residues, and controlling the number of the quaternary ammonium groups covalently grafted on the outer wall of each cucurbit [7] uril and the length of a covalent connecting arm, and on the premise of not destroying the three-strand helical structure of the natural collagen, enadowing the natural collage with the long-acting antibacterial function.

Description

technical field [0001] The invention relates to a method for endowing collagen with a durable antibacterial function by using quaternized cucurbituril on the outer wall, and belongs to the field of biomass materials. Background technique [0002] Collagen has become one of the most critical raw materials in the biomedical material industry due to its good low immunogenicity, biodegradability, hemostasis and repair functions. However, collagen is highly susceptible to bacterial erosion due to its nutrient-rich biomass material. Studies have shown that bacterial metabolism will produce a variety of proteases, thereby destroying the chemical and spatial structure of collagen, reducing its biological activities such as blood coagulation, promoting cell adhesion and proliferation. In addition, microbial metabolites accumulated in collagen devices contain a variety of harmful substances, which pose a threat to the health of users. Therefore, antibacterial and anticorrosion is a ...

Claims

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Application Information

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IPC IPC(8): C07K14/78C07K1/107C08H1/00A01N43/90A01N33/12A01P1/00
CPCC07K14/78C08H1/00A01N43/90A01N33/12Y02A50/30
Inventor 陈意侯德隆吴骁洋颜俊范浩军王忠辉曾琦
Owner SICHUAN UNIV
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