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Root specific promoter promoted aquaporins gene ZMPIP2a expression vector and construction method thereof

A technology for specific promoters and channel proteins, which is used in the introduction of foreign genetic material using vectors, recombinant DNA technology, etc.

Inactive Publication Date: 2013-04-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the plant functional genes such as hormone synthesis, metabolism-related genes, plant growth and development regulation genes are expressed in the above-ground and underground parts of plants, and the simple transgenic or gene silencing methods can only regulate the genes in plants as a whole. expression, cannot specifically regulate the function and development of plant roots

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  • Root specific promoter promoted aquaporins gene ZMPIP2a expression vector and construction method thereof
  • Root specific promoter promoted aquaporins gene ZMPIP2a expression vector and construction method thereof
  • Root specific promoter promoted aquaporins gene ZMPIP2a expression vector and construction method thereof

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Embodiment 1

[0050] A root-specific promoter drives the aquaporin gene ZMPIP2a Expression vector, the starting vector is pGreen0229 plasmid vector, and the root-specific promoter drives the aquaporin gene ZMPIP2a The promoter of the expression vector is the rice root-specific promoter gene EXP18-D , the rice root-specific promoter gene EXP18-D The downstream contains the aquaporin gene ZMPIP2a .

[0051] The root-specific promoter drives the aquaporin gene ZMPIP2a The construction method of expression vector comprises the following steps:

[0052] Ⅰ. Construction of UBQI-Nos-35Sbar-PGreen0229 plasmid vector:

[0053] A, the gene 35Sbar is connected to the PGreen0229 plasmid vector to obtain the 35Sbar-PGreen0229 plasmid vector;

[0054] a. The puc57-35Sbar plasmid was digested with SacI endonuclease to obtain the gene 35Sbar with a SacI restriction site;

[0055] The sequence of the gene 35Sbar with a SacI restriction site is SEQ ID No: 1 in the sequence table; the gene 35Sbar was...

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Abstract

The invention belongs to the field of plant gene engineering and particularly relates to a root specific promoter promoted aquaporins gene ZMPIP2a expression vector and a construction method thereof. The starting vector of the root specific promoter promoted aquaporins gene ZMPIP2a expression vector is PGreen0229 plasmid vector and comprises a rice specific promoter gene EXP18-D and the ZMPIP2a. In the invention, as the expression vector adopts the rice specific promoter gene EXP18-D as a promoter to drive the aquaporins gene ZMPIP2a to express, the expression vector has the potential of specifically changing root form of plants and growing corresponding drought-resistance plants. The survival rate of plants with the promoter is 60 to 70 percent higher than that of reference wild plants under the same drought condition.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering, in particular to a root-specific promoter driving aquaporin gene ZMPIP2a Expression vector and construction method. Background technique [0002] The promoter is the most important factor in the regulation of gene expression, which basically determines whether, when and where a gene is expressed. According to the mode of action and function, promoters can be roughly divided into three categories: constitutive promoters, specific promoters and inducible promoters. At present, the regulatory mechanism of tissue-specific promoters has entered the stage of in-depth research. In addition to the basic structure, such promoters usually have some sequences that regulate specific expression. These sequences provide binding sites for transcription factors. interaction to regulate gene expression. Driven by tissue-specific promoters, gene expression usually only occurs in specific tissues or orga...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/82C12N15/66
Inventor 贾文锁梁莉艳赵艳侠李自超李金杰李冰冰
Owner CHINA AGRI UNIV