Culture system of function strengthened microbe and directional concentration method
A culture system and microbial technology, applied in the field of enrichment and cultivation of nitrogen-removing microorganisms using a continuous culture system, can solve the problems of inability to obtain functionally enhanced microbial communities, bacterial enrichment culture, limited substrates, etc., and achieve strong tolerance and adaptability, increase the enrichment rate, and easy operation
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Embodiment 1
[0023] The nitrous bacteria were cultivated in a continuous feeding full back-mixed reactor at a temperature of 25°C, pH 7.8, dissolved oxygen 1.2mg L -1 Under the conditions of nitrous acid bacteria culture, the initial concentration of ammonia nitrogen in the culture solution is 400mg / L, the concentration of COD is lower than 80mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2- 、K + , Ca 2+ and other metal ions and phosphate ions; denitrifying bacteria are cultivated in a continuous stirred tank reactor at a temperature of 25°C, a pH of 8.0 to 8.5, and a dissolved oxygen of less than 0.2mg L -1Under the conditions of denitrification bacteria culture, the initial concentration of nitrous nitrogen in the culture solution is 380mg / L, the concentration of COD is 1050mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ metal ions and phosphate ions. The directional enrichment of function-enhanced microorganisms...
Embodiment 2
[0025] Using an internal circulation bioreactor at a temperature of 30°C, pH 8.0, dissolved oxygen 0.5mg L -1 The nitrous bacteria are cultivated under certain conditions, the initial ammonia nitrogen concentration of the culture solution is 800mg / L, the COD concentration is lower than 150mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ and other metal ions and phosphate ions; use a continuous stirred tank reactor at a temperature of 30°C, a pH of 8.0-9.0, and a dissolved oxygen of less than 0.5mg L -1 The denitrifying bacteria are cultivated under certain conditions, the initial nitrite nitrogen concentration of the culture medium is 780mg / L, the COD concentration is 2050mg / L, and the culture medium also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ metal ions and phosphate ions. Continuous moving bed filter device is used for directional enrichment of function-enhanced microorganisms. The average growth rate of the two ...
Embodiment 3
[0027] Use a spiral bioreactor at a temperature of 35°C, pH 7.5, dissolved oxygen 2.0mg L -1 The nitrous bacteria are cultivated under certain conditions, the initial ammonia nitrogen concentration of the culture solution is 1200mg / L, the COD concentration is lower than 250mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ and other metal ions and phosphate ions; use a continuous stirred tank reactor at a temperature of 35°C, a pH of 8.0, and a dissolved oxygen of less than 0.1mg·L -1 The denitrifying bacteria were cultivated under certain conditions; the initial nitrite nitrogen concentration of the culture solution was 1100mg / L, the COD concentration was 3050mg / L, and the culture solution also contained a small amount of Fe 2+ , Mg 2- 、K + , Ca 2+ metal ions and phosphate ions. The directional enrichment of function-enhanced microorganisms was carried out by using a continuous moving bed filter device. After culturing for 15 day...
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