Culture system of function strengthened microbe and directional concentration method

A culture system and microbial technology, applied in the field of enrichment and cultivation of nitrogen-removing microorganisms using a continuous culture system, can solve the problems of inability to obtain functionally enhanced microbial communities, bacterial enrichment culture, limited substrates, etc., and achieve strong tolerance and adaptability, increase the enrichment rate, and easy operation

Active Publication Date: 2012-05-16
CHINA PETROLEUM & CHEM CORP +1
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Problems solved by technology

[0008] In order to overcome the shortcomings of poor function, limited substrates, time-consuming and inability to obtain function-enhanced microbial communities in the application of purely cultured microorganisms in engineering systems, solve the problem of enrichment and cultivation of bacteria in the short-cut nitrification and denitrification process , the present invention proposes a continuous operation system that can efficiently cultivate and collect denitrification microorganisms

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  • Culture system of function strengthened microbe and directional concentration method
  • Culture system of function strengthened microbe and directional concentration method
  • Culture system of function strengthened microbe and directional concentration method

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Embodiment 1

[0023] The nitrous bacteria were cultivated in a continuous feeding full back-mixed reactor at a temperature of 25°C, pH 7.8, dissolved oxygen 1.2mg L -1 Under the conditions of nitrous acid bacteria culture, the initial concentration of ammonia nitrogen in the culture solution is 400mg / L, the concentration of COD is lower than 80mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2- 、K + , Ca 2+ and other metal ions and phosphate ions; denitrifying bacteria are cultivated in a continuous stirred tank reactor at a temperature of 25°C, a pH of 8.0 to 8.5, and a dissolved oxygen of less than 0.2mg L -1Under the conditions of denitrification bacteria culture, the initial concentration of nitrous nitrogen in the culture solution is 380mg / L, the concentration of COD is 1050mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ metal ions and phosphate ions. The directional enrichment of function-enhanced microorganisms...

Embodiment 2

[0025] Using an internal circulation bioreactor at a temperature of 30°C, pH 8.0, dissolved oxygen 0.5mg L -1 The nitrous bacteria are cultivated under certain conditions, the initial ammonia nitrogen concentration of the culture solution is 800mg / L, the COD concentration is lower than 150mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ and other metal ions and phosphate ions; use a continuous stirred tank reactor at a temperature of 30°C, a pH of 8.0-9.0, and a dissolved oxygen of less than 0.5mg L -1 The denitrifying bacteria are cultivated under certain conditions, the initial nitrite nitrogen concentration of the culture medium is 780mg / L, the COD concentration is 2050mg / L, and the culture medium also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ metal ions and phosphate ions. Continuous moving bed filter device is used for directional enrichment of function-enhanced microorganisms. The average growth rate of the two ...

Embodiment 3

[0027] Use a spiral bioreactor at a temperature of 35°C, pH 7.5, dissolved oxygen 2.0mg L -1 The nitrous bacteria are cultivated under certain conditions, the initial ammonia nitrogen concentration of the culture solution is 1200mg / L, the COD concentration is lower than 250mg / L, and the culture solution also contains a small amount of Fe 2+ , Mg 2+ 、K + , Ca 2+ and other metal ions and phosphate ions; use a continuous stirred tank reactor at a temperature of 35°C, a pH of 8.0, and a dissolved oxygen of less than 0.1mg·L -1 The denitrifying bacteria were cultivated under certain conditions; the initial nitrite nitrogen concentration of the culture solution was 1100mg / L, the COD concentration was 3050mg / L, and the culture solution also contained a small amount of Fe 2+ , Mg 2- 、K + , Ca 2+ metal ions and phosphate ions. The directional enrichment of function-enhanced microorganisms was carried out by using a continuous moving bed filter device. After culturing for 15 day...

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Abstract

The invention discloses a culture system of function strengthened microbe and a directional concentration method. The culture system comprises a nitrous acid bacterium culture bioreactor, a moving bed adsorption filtering apparatus coupled with the nitrous acid bacterium culture bioreactor, a denitrifying bacterium culture bioreactor and a moving bed adsorption filtering apparatus coupled with the denitrifying bacterium culture bioreactor; the moving bed adsorption filtering apparatuses recover microbial thalli brought out by corresponding bioreactors, and the recovered microbial thalli can be recycled to corresponding bioreactor or be discharged for sale or applied to a correlative sewage disposal system according to requirements. Compared with a prior art, the method of the invention can concentrate two thalli at one time and enhance microbial cultivation quality while reducing production energy consumption. The invention also has advantages of convenient operation, low energy consumption, saved cost and momentarily elution and collection of thalli, etc.

Description

technical field [0001] The invention belongs to the technical field of sewage treatment biology, and in particular relates to a continuous culture system for denitrification microorganisms, in particular to a method for enriching and cultivating denitrification microorganisms using a continuous culture system. Background technique [0002] Traditional secondary biological treatment processes in sewage treatment plants are usually not designed for the removal of high-concentration ammonia nitrogen pollutants, so the removal efficiency of ammonia nitrogen is low. New biological denitrification processes developed in recent years, such as anaerobic ammonium oxidation, short-cut nitrification and denitrification, and simultaneous nitrification and denitrification, are all good ways to solve the problem of ammonia nitrogen pollution. Due to the slow growth of microorganisms that degrade ammonia nitrogen, maintaining a certain amount of biomass is one of the key links in the indus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/06
Inventor 高会杰李志瑞孙丹凤韩建华赵景霞回军
Owner CHINA PETROLEUM & CHEM CORP
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