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Application of leonurine in preparation of medicines used for preventing and treating parkinson disease

A technology for motherwort and Parkinson's disease, which can be applied in drug combinations and nervous system diseases, etc., and can solve problems such as unreported effects

Inactive Publication Date: 2012-05-30
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of leonurine on chronic neurodegenerative diseases has not been reported

Method used

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  • Application of leonurine in preparation of medicines used for preventing and treating parkinson disease
  • Application of leonurine in preparation of medicines used for preventing and treating parkinson disease
  • Application of leonurine in preparation of medicines used for preventing and treating parkinson disease

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Effect test

Embodiment 1

[0023] Example 1 Cell experiment

[0024] In the present invention, the classic neurotoxin 6-OHDA is used to incubate the human nerve cell line SH-SY5Y cells for 24 hours, and the concentration of 8CM198 is selected to be 10-7-10-5mM according to the results of cell survival rate detected by MTT. The treatment drugs were pretreated for 2 hours. Moreover, taking vitamin E as a positive control drug, it was shown that vitamin E 10-5mM had similar effects to SCM19810-6mM and 10-5mM. After clarifying the cytoprotective effect of SCM198, continue to detect the level of LDH in extracellular fluid by colorimetry to evaluate the degree of cell damage; detect SOD activity and ROS generation by colorimetry and fluorescence analysis methods, and evaluate the effect of SCM198 on the antioxidant system; Microscopic analysis of changes in mitochondrial membrane potential to evaluate whether SCM198 has a stabilizing effect on the stability of mitochondrial function; use Hoechst staining, RT...

Embodiment 2

[0025] Embodiment 2 overall experiment

[0026] The present invention adopts the method of intrastriatal injection of 6-OHDA recognized by the international academic circle to prepare the PD rat model. The drug was given by intragastric administration, 3 days in advance, and lasted until 4 weeks after the model was established, and the rotation behavior of the rats induced by apomorphine was counted. The experimental data were analyzed by one-way analysis of variance, and the results showed that SCM198 significantly reduced the number of rotations in PD rats, indicating that SCM198 alleviated the nerve damage caused by 6-OHDA.

[0027] In summary, SCM198 has a protective effect on both the overall PD and the cell model induced by 6-OHDA, and can be used to prepare drugs for the prevention and treatment of PD.

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Abstract

The invention belongs to the traditional Chinese medicine pharmacy field, and relates to an application of leonurine SCM198 in preparation of medicines used for preventing and treating parkinson disease. According to the invention, neurotoxin 6-OHDA induces damage of nerve cells in rats, the result shows that leonurine SCM198 is capable of improving behavior changes of PD model rats, increasing the survival rate of damaged nerve cells, raising antioxidant enzyme superoxide dismutase activity, minimizing excessive generation of active oxygen species, stabilizing mitochondrial membrane potential, minimizing apoptotic cells and regulating the change level of Bax and Bcl2 gene and protein. The result proves that the leonurine SCM198 has protection effect on a PD model, and can be used for preparing medicines for preventing and treating PD.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy. The invention relates to the new medicinal use of motherwortine SCM198, and specifically relates to the application of motherwortine SCM198 in the preparation of a pharmaceutical composition for preventing and treating Parkinson's disease (Parkinson's disease, PD). Background technique [0002] Parkinson's disease is a common chronic neurodegenerative disease, with a prevalence rate of 1.67% among people over 65 years old in my country, and it tends to increase year by year. In advanced cases, the whole body is often stiff and unable to take care of themselves, which is one of the common disabilities in middle-aged and elderly people. At present, dopamine-mimetic drugs are the leading drugs in clinical treatment, which can only relieve symptoms in the early stage of the disease, and will cause severe side effects such as symptom fluctuations and movement disorders. A large n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/235A61P25/16
Inventor 朱依谆史雪茹
Owner FUDAN UNIV
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