Method for direct induction of bulb culture medium by isolated culture of garlic (Allium sativum L.) rachis

An in vitro culture, inflorescence rachis technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of difficult field growing season, low transplant survival rate, connection restriction, etc., and achieve strong axillary bud germination. Potential effect

Inactive Publication Date: 2012-06-20
DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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Problems solved by technology

[0003] The object of the present invention is to solve the problems of difficult domestication of garlic test-tube seedlings, low transplanting survival rate, and difficulty in connecting with field growing seasons, and provides a direct bulb induction medium for in vitro culture of garlic inflorescence axes. The specific technical scheme is as follows:

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  • Method for direct induction of bulb culture medium by isolated culture of garlic (Allium sativum L.) rachis

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specific Embodiment approach 1

[0008] Specific embodiments 1. In this embodiment, every liter of culture medium is synthesized by weight by 1 / 2MS8921.73mg, 90000mg sucrose and the balance is distilled water, and the pH value of the culture medium is 5.8~6.0;

[0009] The composition of described 1 / 2MS consists of potassium nitrate 950mg, ammonium nitrate 825mg, magnesium sulfate 185mg, calcium chloride 220mg, potassium dihydrogen phosphate 85mg, inositol 50mg, boric acid 6.2mg, manganese sulfate 22.3mg, potassium iodide 0.83mg by weight , zinc sulfate 8.6mg, sodium molybdate 0.25mg, glycine 2.0mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, thiamine hydrochloride 0.4mg, pyridoxine hydrochloride 0.5mg, niacin 0.5mg, ferrous sulfate 27.8 mg, EDTA disodium 37.3mg and agar powder 6500mg.

specific Embodiment approach 2

[0010] Specific embodiment two, this embodiment adopts the following test materials and methods: the garlic sprouts of Heilongjiang Province Suiling garlic are collected in the field, after the garlic sprouts are sterilized on the surface, the inflorescence rachis is stripped on the ultra-clean bench and inoculated on the induction medium, and then placed 25~28 ℃, light intensity is 2000LX, light time is 12 hours / every day, after 15 days, count the induction rate of bulb (induce the inflorescence axis number of bulb / the inflorescence axis number of inoculation×100%), the total induction rate of bulb ( The total number of induced bulbs / the total number of inoculated rachis×100%).

[0011] The induction medium is based on 1 / 2MS medium composition, the pH value is 5.8-6.0, and the plant hormone 6-BA (0-2.0) mg / L, NAA (0-0.1) mg / L and 30000-90000 mg / L sucrose concentration, a total of 9 groups of treatments were established.

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Abstract

A method for direct induction of bulb culture medium by isolated culture of garlic (Allium sativum L.) rachis belongs to the technical field of garlic tissue culture and solves the problems that garlic seedlings in test tubes are difficult to domesticate, low in transplant survival rate and difficult to be linked and restricted with field growth seasons. Tissue medium per liter is composed of 1 / 2 MS 8921.73mg, cane sugar 90000mg and the balance distilled water, and the pH value of the culture medium is 5.8-6.0. The method has the advantages that induction rate of bulbs in vitro is 96.8%, and total induction rate of bulbs is 800%. By the method, the problem that test-tube seedlings as connection to field proliferation are difficult in domestication and low in survival rate is solved, induction rate of bulbs in vitro is increased, large-scale production of bulbs in vitro is facilitated, and theoretical basis and key techniques are provided for factory production of bulblet of garlic in vitro.

Description

technical field [0001] The invention relates to the technical field of garlic tissue culture. In particular, it relates to a medium for in vitro culture of garlic inflorescence axis to directly induce bulbs. Background technique [0002] Garlic (Allium sativum L) belongs to the genus Allium in the family Liliaceae. It is an asexually propagated plant. Due to the asexual reproduction in successive years, a large number of viruses accumulate. The main manifestations of garlic infection virus disease are that the garlic heads are getting smaller year by year, the yield is low, and the quality is poor. Generally, the yield reduction reaches 30%. % to 50%, seriously restricting the development of the garlic industry. There have been a lot of studies on the cultivation of virus-free garlic seedlings using shoot tip in vitro culture. The breeding of virus-free seedlings is mostly based on test-tube seedlings as the interface with field multiplication. It is difficult to domesticat...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 宋淑敏刘伟伟潘静张正海王云云高宇
Owner DAQING BRANCH OF HEILONGJIANG ACAD OF SCI
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