Method for rapidly separating quercetin from flos albiziae

A technology of quercetin and albizia julibrissin, which is applied in the field of preparation of traditional Chinese medicine chemical monomers, can solve the problems of difficult to obtain high-purity monomers, normal phase chromatography tailing, long separation time, etc., and achieve high academic value, solvent The effect of less consumption and less toxicity of solvents

Active Publication Date: 2014-07-16
CHENGDU PUSH BIOLOGICAL TECH
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AI Technical Summary

Problems solved by technology

[0006] Quercitrin is a flavonol glycoside compound with poor solubility in conventional reagents and severe tailing in conventional normal phase chromatography. Therefore, the separation time is long and it is difficult to obtain its high-purity monomer
At present, there are few domestic literature reports on the separation and purification of quercetin, and it is the first time that high-efficiency preparative liquid chromatography is used for the separation and purification of quercetin monomer.

Method used

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  • Method for rapidly separating quercetin from flos albiziae
  • Method for rapidly separating quercetin from flos albiziae
  • Method for rapidly separating quercetin from flos albiziae

Examples

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Embodiment 1

[0023] A method for rapidly isolating quercetin from Albizia Julibrissin, characterized in that it is carried out according to the following process steps:

[0024] A. Raw material extraction: put 20kg of Albizia Julibrissin into the extraction tank, add 180L of ethanol solution with a volume fraction of 80%, reflux extraction for 3 times, each time for 2 hours, combine the extracts, concentrate and recover ethanol, and cool the aqueous solution to room temperature;

[0025] B. Macroporous resin enrichment: the extract cooled to room temperature is adsorbed through the activated D101 macroporous adsorption resin column at a speed of 4BV. The volume of the resin bed is about 8L, and then 25L of ethanol solution with a volume fraction of 60% Elute the impurities, then elute the target substance with 20L ethanol solution with a volume fraction of 80%, collect the eluate, concentrate it to about 2L, let it stand for crystallization, filter, collect the crystals, and obtain the crud...

Embodiment 2

[0030] A method for rapidly isolating quercetin from Albizia Julibrissin, characterized in that it is carried out according to the following process steps:

[0031] A. Raw material extraction: put 50kg of Albizia Julibrissin into the extraction tank, add 400L of ethanol solution with a volume fraction of 70%, reflux extraction for 3 times, each time for 2 hours, combine the extracts, concentrate and recover ethanol, and cool the aqueous solution to room temperature;

[0032] B. Macroporous resin enrichment: Adsorb the cooled extract through an activated AB-8 macroporous adsorption resin column at a rate of 5BV. The volume of the resin bed is about 20L, and then use 80L of ethanol solution with a volume fraction of 50%. Elute the impurities, then elute the target substance with 70L ethanol solution with a volume fraction of 75%, collect the eluate, concentrate it to about 7L, let it stand for crystallization, filter, collect the crystals, and obtain the crude quercitrin;

[003...

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Abstract

The invention discloses a method for rapidly separating quercetin from Albizia Julibrissin, belonging to the technical field of traditional Chinese medicines. The method is carried out according to the following process steps: A. Raw material extraction: reflux extraction with 60-80% ethanol solution for 3 times, each time for 2 hours; B. Macroporous resin enrichment: pass through the activated macroporous resin at a speed of 2-5BV Porous adsorption resin column for adsorption, macroporous adsorption resin is selected from D101 type or AB-8 type; C. Efficient preparation of liquid phase separation: the chromatographic column packing is C18 packing, and the mobile phase is methanol solution with a volume fraction of 30-40%. Wavelength 254nm; D. Product recovery: The precipitated crystals were dried at 60-80°C for 6-8 hours to obtain quercitrin monomer with a purity of more than 99%. The method of the invention is simple and fast, has less solvent consumption, large output, high yield, economical and environmental protection, is suitable for industrial production, and has high economic value and academic value.

Description

technical field [0001] The invention relates to a method for preparing a chemical monomer of traditional Chinese medicine, which belongs to the technical field of traditional Chinese medicine. Specifically, the invention relates to a method for rapidly separating high-purity quercitrin monomer from the traditional Chinese medicinal material Albizia Julibrissin. Background technique [0002] Albizia julibrissin belongs to the leguminosae (Leguminosae) genus ( Albizia ) plant Albizia juliensis ( Albizia julibrissin Durazz.) dry inflorescences or flower buds, the inflorescences are commonly called "Albizia julibrissin", and the flower buds are commonly called "Albizia julibrissin rice". Nature and flavor are sweet, flat, GUIXIN, Liver Channel. It has the effect of relieving depression and calming the nerves. For restlessness, depression and insomnia. It is recorded in the "Chinese Pharmacopoeia" of the Ministry of History. [0003] Quercitrin (Quercitrin), that is, querc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 夏柯文焕松陈军郭建华刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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