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Method for converting Qula acid-method casein into chymosin casein

A technology of casein and chymosin, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as low yield, unqualified quality, and many impurities, so as to improve utilization rate and expand application , to avoid the bad effect of coagulation

Active Publication Date: 2014-05-14
甘肃华羚乳品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the extensive production method used in Qula, there are many impurities and sour odor. When it is directly used to prepare rennet casein, it often has problems such as poor coagulation, low yield, bad smell, and poor quality.

Method used

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  • Method for converting Qula acid-method casein into chymosin casein
  • Method for converting Qula acid-method casein into chymosin casein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] a) Dissolution Make 10kg of raw material acid-process casein into a solution with a mass concentration of 5%, adjust the pH to 5.5 with NaOH, and fully stir to dissolve;

[0031] b) Activation Add 1.0% CaCl of raw material mass to the dissolved casein 2 , stir well and adjust the temperature to 39°C;

[0032] c) Curd Add 25g of microbial rennet with an activity of 2000U / g, and the curd will appear in 17 minutes;

[0033] d) Cutting When the cut of the curd is smooth, cut the curd into 1cm 3 small pieces;

[0034] e) Inactivation After cutting, heat up to 70°C and keep stirring for 1 min;

[0035] f) Filtration After inactivation, filter to remove water, leaving the precipitate wet casein;

[0036] g) Washing Wash the filtered wet casein twice with warm water at 40°C;

[0037] h) Dehydration After washing, squeeze and dehydrate to a moisture content of 45%;

[0038] i) Granulation After dehydration, use a 30-mesh screen to granulate through a granulator;

[0039] ...

Embodiment 2

[0041] a) Dissolution Make 10kg of acid-process casein as a raw material into a solution with a mass concentration of 20%, adjust the pH to 6.0 with NaOH, and fully stir to dissolve;

[0042] b) Activation Add 1.2% CaCl of raw material mass to the dissolved casein 2 , stir well and adjust the temperature to 45°C;

[0043] c) Curd Add 25g of microbial rennet with an activity of 2000U / g, and the curd will appear in 15 minutes;

[0044] d) Cutting When the cut of the curd is smooth, cut the curd into 3cm 3 small pieces;

[0045] e) Inactivation After cutting, heat up to 60°C and keep stirring for 3 minutes;

[0046] f) Filtration After inactivation, filter to remove water, leaving the precipitate wet casein;

[0047] g) Washing Wash the filtered wet casein with warm water at 45°C for 5 times;

[0048] h) Dehydration After washing, centrifuge and dehydrate to a moisture content of 50%;

[0049] i) Granulation After dehydration, use a 30-mesh screen to granulate through a gra...

Embodiment 3

[0052] a) Dissolution Make 10kg of acid-process casein as a raw material into a solution with a mass concentration of 20%, adjust the pH to 6.5 with NaOH, and fully stir to dissolve;

[0053] b) Activation Add 1.6% CaCl of raw material mass to the dissolved casein 2 , stir well and adjust the temperature to 45°C;

[0054] c) Add vitality 5000U / g calf rennet enzyme 10g to curd, and the curd will appear in 30min;

[0055] d) Cutting When the cut of the curd is smooth, cut the curd into 3cm 3 small pieces;

[0056] e) Inactivation After cutting, heat up to 70°C and keep stirring for 3 minutes;

[0057] f) Filtration After inactivation, filter to remove water, leaving the precipitate wet casein;

[0058] g) Washing Wash the filtered wet casein with warm water at 45°C for 5 times;

[0059] h) Dehydration After washing, centrifuge and dehydrate to a moisture content of 45%;

[0060]i) Granulation After dehydration, use a 60-mesh screen to granulate through a granulator;

[00...

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Abstract

The invention discloses a method for converting Qula acid-method casein into chymosin casein. The method adopts Qula acid-method casein as a raw material, and comprises steps of dissolution, activation, coagulation, cutting, inactivation, washing, dehydration, and drying. The invention performs enzyme method conversion by using traditional Qula acid-method casein, produces chymosin casein with a higher added value, avoids problems of poor coagulation, low yield, bad smell, and poor quality that does not reach the standard for direct preparation of chymosin casein by Qula, extends the applications of Qula acid-method casein, and increases the utilization rate of expensive chymosin.

Description

technical field [0001] The invention relates to a method for converting rennet casein with kojic acid method casein, which belongs to the technical field of dairy products. Background technique [0002] Casein, also known as casein, is a phosphorus-containing protein in animal milk, accounting for 80% of milk protein in milk, and can be used in food additives, casein gum, cosmetics and other fields. There are two ways to prepare it. One method is to add acid to adjust the pH after skimming the milk, so that the casein micelles lose their charge and coagulate and precipitate. The casein obtained by this method is called acid casein. There is little difference in the casein obtained from different types; another method is to react milk with rennet to form a coagulated precipitate, which is called rennet casein. [0003] Rennet casein is a kind of casein with high protein content, which has unique functional characteristics such as excellent dyeing adhesion ability, strong ant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435C07K1/30
Inventor 纪银莉甘伯中高维东何潇宋礼张玉平葛静微苏艳萍唐兴宇颉瑜卢玮玮周正刚马振贵
Owner 甘肃华羚乳品股份有限公司