Crude polysaccharide extract of mokara root and application
A technology of Morse's orchid root and crude polysaccharide, which is applied in the field of Morse's orchid root crude polysaccharide extract, to achieve the effect of excellent moisturizing and satisfying safety
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Embodiment 1
[0031] Example 1: Preparation of Morse blue root crude polysaccharide.
[0032] Take 100.0g of washed, dried and chopped Mohs orchid roots, use 2000ml of distilled water to sonicate at 60℃, ultrasonic frequency is 45kHz, ultrasonic power ratio is 80%, heat for 1h, filter, repeat the extraction of filter residue twice, and combine the filtrates three times Concentrate under reduced pressure at 80°C to 100ml, add 5 times the volume of 95% (v / v) ethanol to the concentrate to make the volume percentage of ethanol reach 80%, mix well, refrigerate at 4°C for 12 hours, and then remove For the supernatant, centrifuge the precipitate (3000 r / min, 10 min), take the precipitate and add distilled water to dissolve it, let it stand, and centrifuge (3000 r / min, 10 min), take the supernatant, add 5 times the volume of 95% (v / v) wash the precipitate with ethanol, add distilled water to dissolve the precipitate, then add 5 times the volume of absolute ethanol and stir evenly, then centrifuge (...
Embodiment 2
[0033] Example 2: Preparation of Morse blue root crude polysaccharide.
[0034] 100.0 g of washed, dried and chopped Morsemen's orchid roots were ultrasonicated with 4000 ml of distilled water at 50°C, the ultrasonic frequency was 45kHz, and the ultrasonic power ratio was 70%. Heat for 2 hours, filter, and extract the filter residue twice, combine the filtrates for three times and concentrate under reduced pressure at 70°C to 100ml, add 95% (v / v) ethanol to the concentrated solution to make the volume percentage of ethanol reach 80%, and mix well Afterwards, refrigerate at 4°C for 24 h, then remove the supernatant, centrifuge the precipitate (3000 r / min, 15 min), dissolve the precipitate in distilled water, let stand, centrifuge (3000 r / min, 10 min), and take Add 8 times the volume of 95% (v / v) ethanol to the supernatant to wash the precipitate, add distilled water to dissolve the precipitate, then add 8 times the volume of absolute ethanol to stir evenly, then centrifuge (300...
Embodiment 3
[0035] Example 3: Preparation of Morse blue root crude polysaccharide.
[0036] 100.0 g of washed, dried, and chopped Morsemen's orchid roots were ultrasonicated with 1000 ml of distilled water at 60°C, the ultrasonic frequency was 45 kHz, and the ultrasonic power ratio was 75%. Heat for 2 hours, filter, and extract the filter residue twice, combine the filtrates for three times and concentrate under reduced pressure at 80°C to 100ml, add 90% (v / v) ethanol to the concentrated solution to make the volume percentage of ethanol reach 80%, and mix well Afterwards, refrigerate at 4°C for 24 h, then remove the supernatant, centrifuge the precipitate (3000 r / min, 15 min), dissolve the precipitate in distilled water, let stand, centrifuge (3000 r / min, 10 min), and take Add 4 times the volume of 90% (v / v) ethanol to the supernatant to wash the precipitate, add distilled water to dissolve the precipitate, then add 4 times the volume of absolute ethanol to stir evenly, then centrifuge (3...
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