Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof

A technology of cotton verticillium wilt and pseudomonas, applied in the field of pseudomonas, can solve the problems that the disease control methods and chemicals are difficult to achieve the ideal control effect, difficult to prevent and control, difficult to find anti-source materials, etc., and achieve good biological pesticides Development prospect, remarkable effect

Inactive Publication Date: 2012-07-04
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Verticillium wilt of cotton is a soil-borne vascular disease. It is difficult to control and find good resistant materials. Therefore, it has not

Method used

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  • Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof
  • Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof
  • Pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 Obtaining of Pseudomonas 841P-3

[0027] 1.1 Isolation of bacteria

[0028] Take healthy budding cotton M-8124-1159 flower roots, take the 5-10cm of the taproot with a sterilizing knife, soak in 70% alcohol for 1min, sterilize the surface of the sample with 1.05% sodium hypochlorite, and the sterilization time is 15-18min. Take 1.00 g of the material that has been thoroughly sterilized and place it in a sterile mortar, add sterile PBS buffer, and grind it. After standing for 15 minutes, add the buffer to make the volume to 10 mL, take 1 ml of the separating solution and apply 0.1 ml after gradient dilution. For the prepared S2 (agar 18g, sucrose 10g, glycerol 10ml, casein 5g, sodium bicarbonate 1g, MgSO 4 ·7H 2 O 1g, K 2 HPO 4 2.3g, 1.2g sodium lauroyl sarcosinate, deionized water to a volume of 1L) medium, used for the isolation of Pseudomonas, in three parallel groups, cultured at 28°C for 2 to 3 days, counting the number of colonies. Pick a single colony accor...

Embodiment 2

[0036] Example 2 Study of antagonistic mechanism:

[0037] Pick out the hyphae around the antagonistic Pseudomonas 841P-3 (CGMCC No.5583) in the opposite culture and collect the spores around the antagonistic treatment to observe the hypha growth and spore germination. The results showed that the hyphae of Verticillium dahliae V107 surrounding Pseudomonas 841P-3 (CGMCC No. 5583) appeared deformed, enlarged, grew abnormally, spores ruptured, and spore germination was inhibited ( Figure 4 , 5).

Embodiment 3

[0038] Example 3 Verification of induced resistance

[0039] 3.1 Improve cotton root resistance

[0040] Preparation of bacterial suspension: Inoculate Pseudomonas 841P-3 (CGMCC No.5583) strain in liquid LB, incubate at 37℃ for 24h, centrifuge to remove the supernatant, resuspend in sterile water, and dilute to OD 600 = 0.1 bacterial suspension. After soaking the sterilized cotton seeds for 30 minutes and culturing them at 28°C for 48 hours, they were transferred to a PDA plate with Verticillium dahliae V107 grown for 2 days, and the damage was observed after a total of 7 days. The results showed that the root damage of Pseudomonas 841P-3 (CGMCC No. 5583) after seed soaking was significantly less than that of the roots treated with water. Image 6 .

[0041] 3.2 Improve leaf resistance

[0042] The concentration of bacterial suspension is OD 600 =0.2 Pseudomonas 841P-3 (CGMCC No.5583) After irrigating the cotton plants with 2 true leaves for 48 hours, take the true cotton leaves and ...

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Abstract

The invention belongs to the technical field of biopesticides and relates to pseudomonas sp. 841P-3 for preventing cotton verticillium wilt and use thereof. The pseudomonas sp. 841P-3 was preserved in China General Microbiological Culutre Collection Center in December 13th, 2011. The collection number of the pseudomonas sp. 841P-3 is CGMCC No.5583. The invention also discloses the use of the pseudomonas sp. 841P-3 with the collection number being CGMCC No.5583 in prevention and control of cotton verticillium wilt. Through microbial fermentation, lots of pseudomonas sp. 841P-3 strains can be obtained. The strains can produce resistance to induction system, are safe to human beings, animals and crops, are environment-friendly, have obvious cotton verticillium wilt prevention and control effect and good biopesticide development prospect.

Description

Technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a Pseudomonas strain for preventing and treating Verticillium wilt of cotton and its application. Background technique [0002] Cotton Verticillium wilt (cotton Verticillium wilt) is a fungal vascular disease caused by Verticillium dahliae infection and is one of the most serious diseases of cotton. The mycelium of Verticillium dahliae can produce a large number of micro sclerotia. These micro sclerotia can survive in the soil for a long time, have a wide range of hosts and have many transmission channels, which seriously threaten cotton production and cause serious losses to my country's cotton production. So far there is no effective control method. Cotton verticillium wilt is a soil-borne vascular bundle disease. It is difficult to prevent and control and it is difficult to find a good source of resistance. Therefore, it has not been possible to cultivate new cotton va...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
Inventor 唐灿明赵明文韩琴李顺鹏
Owner NANJING AGRICULTURAL UNIVERSITY
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