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Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same

A technology of elderberry and tea inchworm, which is applied in the fields of tea inchworm breeding and tea inchworm nuclear polyhedrosis virus propagation, can solve the problems of inability to produce EcobNPV on a large scale and high cost, and reduce premature death or pupation, strong virulence, and Reduce the effect of replacement

Inactive Publication Date: 2012-07-11
INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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Problems solved by technology

[0009] The purpose of the present invention is to overcome the defects and problems of being unable to produce EcobNPV on a large scale and having high costs in the prior art, to provide a kind of elderflower that can produce EcobNPV on a large scale and to raise tea geometrids with lower cost, and to use it to proliferate EcobNPV method

Method used

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  • Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same
  • Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same
  • Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same

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Embodiment 1

[0072] The method for raising the tea looper with elderberry, the method may further comprise the steps in turn:

[0073] Step 1: First collect the pupae of healthy tea inchworm (Ectropis obliqua Warren), and then place them in sterilized insect culture containers. Each insect culture container holds 400–500 pupae, and raise them at room temperature until they become adults Feathering;

[0074] Step 2: After the above pupae emerge, first put 10% sucrose solution in the insect-raising container to supplement nutrition for the adults, and then cover the insect-raising container with high-density gauze for the mated females to lay eggs on the surface;

[0075] Step 3: After the larvae hatch from the eggs, start to feed the tea geometrid larvae with elderflowers (Sambucus chinensis). The hatched larvae feed on the elderberry grown in water in the narrow-mouth bottle, and when the inoculation amount of the elderberry in each narrow-mouth bottle reaches 80-120 heads, then the fine-...

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Abstract

The invention relates to a method of raising ectropis oblique warren through sambucus chinensis and an Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing the same. The methods provided by the invention are characterized in that the sambucus chinensis rather than tea leaves are used for raising the sambucus chinensis, various raising operations and propagation operations are matched to improve the ectropis oblique warren raising effect and the EcobNPV propagation effect, the problems that the traditional feed can not be used for raising the ectropis oblique warren yearly and succeedingly can be overcome, and the difficulty of EcobNPV propagation on large scale can be solved. The design has the advantages of low cost, lower operation difficulty, high EcobNPV propagation effect and strong EcobNPV virulence, and can meet the requirement of scaled EcobNPV production.

Description

technical field [0001] The present invention relates to the field of tea geometrid breeding and tea geometrid nuclear polyhedrosis virus propagation, in particular to raising tea geometrids with elderflowers and a method for multiplying EcobNPV with them, and is specifically applicable to the method of raising tea geometrids with elderflowers to realize the annual scale Cultured propagation of EcobNPV. Background technique [0002] The tea inchworm Ectropis obliqua Warren, belonging to Lepidoptera and Geometridae, is one of the major tea tree pests in my country. It is distributed throughout the major tea-producing areas, especially in the middle and lower reaches of the Yangtze River, especially in Zhejiang, Jiangsu, Anhui, and Hubei. The larvae eat leaves and cause damage, often causing bare tea bushes, seriously affecting the tree vigor and tea yield of tea trees. In addition to harming tea trees, they also feed on plants such as soybeans, sunflowers, and pomegranates. ...

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Application Information

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IPC IPC(8): A01K67/033C12N7/00C12R1/93
Inventor 龚自明刘明炎毛迎新谭荣荣
Owner INST OF FRUIT & TEA HUBEI ACAD OF AGRI SCI
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