Porcine seneca valley virus vaccine and preparation method thereof

A virus vaccine, virus technology, applied in biochemical equipment and methods, viruses, vaccines, etc., can solve problems such as slow weight gain, economic loss, high mortality, etc.

Active Publication Date: 2021-02-02
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] It was first discovered in the United States in 2002, and then the disease broke out almost simultaneously around the world, proving that it has the possibility of further expanding the scope of the outbreak, especially as a new disease, there is no corresponding vaccine and appropriate treatment measures, once the epidemic occurs, the consequences will be caused Difficult to estimate
The disease is indistinguishable from vesicular diseases s

Method used

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  • Porcine seneca valley virus vaccine and preparation method thereof
  • Porcine seneca valley virus vaccine and preparation method thereof
  • Porcine seneca valley virus vaccine and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1——The gene characteristic of SVV-SD-2018 strain ORF fragment

[0066] The structural protein of the SVV-SD-2018 strain is the ORF region. The ORF fragment was amplified and sequenced by PCR using 5 pairs of primers. The homology comparison showed that the amino acid sequence of this fragment was compared with domestic reference strains / epidemic strains. Mutations occurred at positions 165, 495, 575, 770, 1119, 1476, 1609, 1854, 2065, 2104 and 2109; , 1119, 1469, 1476, 1609, 1854, 2065, 2104 and 2109 were mutated.

Embodiment 2

[0067] Embodiment 2——Preparation of poisonous seeds for production

[0068] Add the SVV-SD-2018 virus seed into the well-growing BHK-21 cell suspension culture at the ratio of MOI=0.01~0.5, culture at 37°C, harvest when the cell viability rate is lower than 50%, quantitatively aliquot, and cryopreserve. Indicate the date of harvest, algebra of poisonous species, etc. The virus content remained stable at 10 9.5 TCID 50 / mL or more.

Embodiment 3

[0069] Embodiment 3——Preparation of Porcine Seneca Virus Inactivated Vaccine

[0070] 1. Cell Culture and Inoculation

[0071] (1) Shake flask culture process

[0072] 1) Effects of different multiplicity of infection on virus content The initial cell density was 2.5×10 6 Cells / mL BHK-21 cells were inoculated with SVV-SD-2018 strain at MOI of 0.01, 0.05, 0.1, and 0.5, respectively, and samples were taken 24 hours, 36 hours, and 48 hours after inoculation to determine the virus content.

[0073] 2) Effect of cell density on virus content when inoculated BHK-21 cells were inoculated into shake flasks, when the cell density was 2.0×10 6 cells / mL, 2.5×10 6 cells / ml, 3.0×10 6 cells / mL, 3.5×10 6 cells / mL, 4.0×10 6 Cells / mL, the SVV-SD-2018 strain was inoculated according to MOI=0.05, samples were taken 24 hours, 36 hours, and 48 hours after inoculation, and the virus content was determined.

[0074] 3) Effect of different harvesting times on virus content Inoculate shake flas...

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Abstract

The invention relates to a porcine seneca valley virus vaccine and a preparation method thereof. A porcine seneca valley virus SVV-SD-2018 isolated strain is used for preparing a porcine seneca valleyvirus inactivated vaccine, the ORF of the strain has unique amino acid site arrangement, the infectivity to a suspended cell strain BHK is greatly enhanced, the virus production efficiency is high, and the strain has good immunogenicity. The SVV-SD-2018 strain is proliferated by a suspension cell BHK, and an adjuvant is added to a purified antigen to prepare the porcine an adjuvant inactivated vaccine. The vaccine is safe, and efficacy test results show that the inactivated vaccine has no adverse reaction after being used for immunizing pigs, and can generate good protective force. The resultshows that the vaccine prepared from the strain is safe and effective, and can be used for preventing porcine seneca valley virus disease.

Description

technical field [0001] The invention relates to a porcine Seneca virus vaccine and a preparation method thereof, belonging to the technical field of veterinary biological products. Background technique [0002] Seneca virus (Seneca Valley Virus, SVV) belongs to Picornaviridae, Senecavirus genus. The clinical symptoms are mainly blisters and ulcers in the nose and hooves, similar to FMDV, VSV and SVD infection symptoms; infected piglets have a certain mortality rate (30% to 70%). [0003] It was first discovered in the United States in 2002, and then the disease broke out almost simultaneously around the world, proving that it has the possibility of further expanding the scope of the outbreak, especially as a new disease, there is no corresponding vaccine and appropriate treatment measures, once the epidemic occurs, the consequences will be caused Difficult to estimate. Therefore, the vaccine research of this disease is imminent, and the application market is extensive. ...

Claims

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Application Information

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IPC IPC(8): A61K39/125A61P31/14C12N7/00C12N7/02C12R1/93
CPCA61K39/12A61K39/39A61P31/14C12N7/00A61K2039/552A61K2039/5252C12N2770/32021C12N2770/32034C12N2770/32051Y02A50/30
Inventor 宋丽丽张连秀于宗幸张广宋晓飞段小波王蕾徐龙涛
Owner QILU ANIMAL HEALTH PROD
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