Double-mode imaging and medicine-loading integrated nano medicine carrier and preparation method thereof

A nano-drug carrier and dual-mode imaging technology, which is applied to medical preparations of non-active ingredients, drug combinations, drug delivery, etc., to achieve the effects of reducing toxic side effects and damage to normal tissues, simple process, and high accuracy

Inactive Publication Date: 2012-07-11
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
View PDF2 Cites 18 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the above-mentioned expected prospects, the purpose of the present invention is to provide a nano-drug carrier that integrate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Double-mode imaging and medicine-loading integrated nano medicine carrier and preparation method thereof
  • Double-mode imaging and medicine-loading integrated nano medicine carrier and preparation method thereof
  • Double-mode imaging and medicine-loading integrated nano medicine carrier and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Dissolve doxorubicin hydrochloride in water, add a small amount of triethylamine to extract doxorubicin into dichloromethane. Then carboxy-terminated poly(lactic-co-glycolic acid) (hereinafter referred to as PLGA, Mw=10000, LA:GA=75:25) and upconversion luminescent nanocrystals NaGdF4:Yb, Er were dissolved in dichloromethane. Take a certain amount of each of the three solutions and make 1ml of dichloromethane solution, in which 8mg of PLGA, 4mg of NaGdF4:Yb, Er and 500μg of doxorubicin are dissolved, and this dichloromethane solution is added dropwise to 10ml containing 1 In the aqueous solution of % PVA, stir at 16000rpm for 10min to form a stable O / W emulsion, then gently stir in a water bath at 40°C for 6h to volatilize dichloromethane and solidify the nanospheres. The nanospheres in the suspension are collected by centrifugation, washed several times with double distilled water, and freeze-dried to obtain the functional nanometer drug carrier.

Embodiment 2

[0031] Example 2: Paclitaxel, carboxy-terminal PLGA (Mw=10000, LA:GA=75:25) and upconversion luminescent nanocrystals NaGdF4:Yb, Er were dissolved in dichloromethane, respectively. Take a certain amount of each of the three solutions to make 1ml of dichloromethane solution, so that 8mg of PLGA, 4mg of NaGdF4:Yb, Er and 500μg paclitaxel are dissolved in it, and inject the dichloromethane solution into 10ml of 1% PVA-containing aqueous solution , stirred at 16000rpm for 10min to form a stable O / W emulsion, then gently stirred in a water bath at 40°C for 6h to volatilize dichloromethane and solidify the nanospheres. The nanospheres in the suspension are collected by centrifugation, washed several times with double distilled water, and freeze-dried to obtain the functional nanometer drug carrier.

Embodiment 3

[0032] Example 3: Paclitaxel, carboxy-terminal PLGA (Mw=10000, LA:GA=75:25) and upconversion luminescent nanocrystals NaGdF4:Yb, Er were dissolved in dichloromethane, respectively. A certain amount of each of the three solutions was prepared into 1 ml of dichloromethane solution, in which 8 mg of PLGA, 4 mg of NaGdF4:Yb, Er and 500 μg of paclitaxel were dissolved. Weigh 56mg of SDS and dissolve it in 10ml of deionized water, then inject this 1ml of dichloromethane solution into the SDS aqueous solution, stir at 16000rpm for 10min to form a stable O / W emulsion, then gently stir overnight to volatilize the dichloromethane and solidify nanospheres. The nanospheres in the suspension were collected by centrifugation, washed several times with double distilled water, and freeze-dried to obtain functional composite PLGA nanospheres.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Sizeaaaaaaaaaa
Login to view more

Abstract

The invention relates to a double-mode imaging and medicine-loading integrated nano medicine carrier and a preparation method thereof. The nano medicine carrier is characterized in that nanoparticles are formed by wrapping functional nanocrystal and anti-tumor medicines through using biodegradable macromolecular polymers; the size of the nano medicine carrier is less than 500 nm; and the nano medicine carrier has a smooth surface and is regular in particle size. The nano medicine carrier is prepared by at least one of a precipitation method, a micellar method and a microemulsion method. The microemulsion method comprises the following steps of: preparing the functional nanocrystal, the anti-tumor medicines and the biodegradable macromolecular polymers into liquid respectively; mixing and stirring to form microemulsion; and curing nanospheres with stirring, centrifugally separating the nanospheres, cleaning and freeze-drying to prepare the double-mode imaging and medicine-loading integrated nano medicine carrier. According to the technical scheme, double functions of tumor fluorescence diagnosis and magnetic resonance imaging of the nano medicine carrier are realized; sustained release of the medicines and targeted medicine supply are realized; and toxic or side effect and damage to the normal tissue caused by high-dose multiple administration are greatly reduced.

Description

technical field [0001] The invention relates to the application of a nano-medicine carrier and a preparation method thereof, in particular to a nano-medicine carrier capable of simultaneously carrying a dual-mode imaging material and an antitumor drug and a preparation method thereof. Background technique [0002] In the field of biomedicine, the development of imaging technology with high spatial resolution, high sensitivity and non-invasiveness has become a research hotspot in the field of nanobiomedicine. The imaging technologies currently used in clinical medical diagnosis include: X-ray computed tomography (CT), Positron emission tomography, magnetic resonance imaging, etc. However, the spatial resolution of these medical imaging technologies still cannot meet people's needs, and the imaging quality is closely related to the properties of contrast agents. A challenging research work. As we all know, fluorescence imaging technology has high sensitivity. For example, co...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K49/18A61K49/12A61K49/00A61K47/30A61K9/14A61P35/00
Inventor 王新杨辉朱毅敏
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap