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Protein AtLPT4 related to deficient-phosphorus stress tolerance of plants, and coding gene and application thereof

A technology for low-phosphorus tolerance and plants, which is applied to the protein AtLPT4 related to plant resistance to low-phosphorus stress and its encoding gene and application field, which can solve problems such as unclear functions

Active Publication Date: 2014-01-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It is now known that the Arabidopsis genome contains more than 1500 genes encoding transcription factors, including 74 WRKY genes. The role of these WRKY genes in plant response to low phosphorus stress is still unclear

Method used

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  • Protein AtLPT4 related to deficient-phosphorus stress tolerance of plants, and coding gene and application thereof
  • Protein AtLPT4 related to deficient-phosphorus stress tolerance of plants, and coding gene and application thereof
  • Protein AtLPT4 related to deficient-phosphorus stress tolerance of plants, and coding gene and application thereof

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Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, acquisition and detection analysis of transgenic AtLPT4 plants

[0048] 1. Obtaining transgenic AtLPT4 Arabidopsis

[0049] 1. Acquisition of cDNA

[0050] Total RNA was extracted from Arabidopsis thaliana (Columbia type (Col-0 ecotype), (purchased from the American Arabidopsis Biological Resource Center, ABRC)) seedlings for 10 days, and reverse-transcribed into cDNA. The cDNA was Template, utilize respectively to be provided with a pair of primers of XbaI and KpnI restriction site to carry out PCR amplification, obtain the cDNA full-length of the gene AtLPT4 of plant resistance to low phosphorus stress related protein.Primer sequence is as follows:

[0051] Primer1: 5′-GC tctaga ATGGAGGATAGGAGGTGTGA-3' (XbaI);

[0052] Primer2: 5′-gg ggtacc TCATTCCTTCAAGCAAAAGGGA-3' (KpnI).

[0053] The high-fidelity Pyrobest DNA polymerase from Takara Company was used for amplification. The amplification system was 50 μL, containing 5.0 μL of 10×Pyrobest PCR buff...

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Abstract

The invention discloses protein AtLPT4 related to deficient-phosphorus stress tolerance of plants, and coding gene and an application thereof. The protein provided by the invention is protein shown as 1) or 2), wherein 1) protein consists of amino acid sequence shown in a sequence 2 of a sequence table; and 2) protein is formed by substitution and / or deficiency and / or addition of one or more amino acid residues of amino acid residue sequence of the sequence 2 in the sequence table, is related to the deficient-phosphorus stress tolerance of the plants and is derived from 1). The coding gene AtLPT4 of the protein disclosed by the invention is applied to a target plant, and then a transgenic plant is obtained. The transgenic plant obtained by the invention shows phosphorus nutrition traits. The gene has an important significance in culturing new varieties of plants with deficient-phosphorus stress tolerance and studying the deficient-phosphorus tolerance of the plants.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a plant low phosphorus stress-related protein AtLPT4 and its encoding gene and application. Background technique [0002] Phosphorus is one of the essential macroelements for plants and is involved in the growth and development of plants. The phosphorus concentration in plant cells is generally at the mM level, while the soil phosphorus concentration is extremely low, generally below 10 μM, so plants often face low phosphorus stress, and soil phosphorus deficiency has become an important limiting factor limiting agricultural production. Phosphate rock is a non-renewable resource, and a large amount of phosphate fertilizer will cause environmental pollution. Therefore, it has become an important research work to understand the response mechanism of plants to low phosphorus stress, improve the plant's own tolerance to low phosphorus stress, and further improve crop yield, and has attr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C12N15/29C12N5/10C07K14/415C12N15/82
Inventor 陈益芳武维华王慧徐谦段军叶
Owner CHINA AGRI UNIV