Ammonia (ammonia ion) content determination method and ammonia (ammonia ion) diagnosis/determination kit
A technology of ammonia ions and reagents, which is applied in the field of medical/food testing and determination, and can solve the problems of undiscovered blood ammonia determination methods, etc.
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Embodiment 1
[0132] 1) Preparation of standard samples
[0133] Dissolve a certain amount of ammonia salt in water or buffer solution, and then adjust the ammonia concentration to 100 micromole / liter;
[0134] 2) Pretreatment of samples to be tested
[0135] Plasma samples are used as samples to be tested without pretreatment;
[0136] 3) Blank sample
[0137] Described water or buffer solution are used as blank sample, and its ammonia concentration is 0 micromole / liter;
[0138] B. Preparation of reagent solution:
[0139] The ammonia diagnosis / measurement reagent of the present embodiment is a single-dose reagent, which contains:
[0140] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0141] Glycerol 1mol / L
[0142] NADPH0.25mmol / L
[0143] Ammonia kinase 16000U / L
[0144] Carbamyl phosphate synthase 18000U / L
[0145] Hydrogen cyanide synthase 12000U / L
[0146] Adenosine triphosphate 5mmol / L
[0147] Glutamic acid 5mmol / L
[0148] Carbamyl phosphate 5mmol / ...
Embodiment 2
[0164] 1) Preparation of standard samples
[0165] A certain amount of ammonia salt is dissolved in water, and then the concentration of ammonia (ammonia ion) is adjusted to 100 micromole / liter, as a standard sample;
[0166] 2) Preparation of samples to be tested
[0167] Plasma samples are used as samples to be tested without pretreatment;
[0168] 3) Blank sample
[0169] Described water is used as blank sample, and its ammonia (ammonia ion) concentration is 0 micromole / liter;
[0170] B. Preparation of reagent solution:
[0171] Ammonia (ammonia ion) diagnostic / determination reagent is a two-dose reagent, which contains:
[0172] Reagent 1 with the following composition:
[0173] Phosphate buffer 100mmol / L
[0174] NADH0.25mmol / L
[0175] Adenosine triphosphate 5mmol / L
[0176] Glutamic acid 5mmol / L
[0177] Carbamyl phosphate 5mmol / L
[0178] Monovalent phosphate 5mmol / L
[0179] Hydrogen cyanide 5mmol / L;
[0180] Reagent 2 with the following composition:
[...
Embodiment 3
[0203] The mode of operation of this embodiment is the same as embodiment 2, but this embodiment:
[0204] B. Preparation of reagent solution:
[0205] Ammonia (ammonia ion) diagnostic / assay reagent is a three-dose reagent that contains:
[0206] Reagent 1 with the following composition:
[0207] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0208] NADH0.25mmol / L
[0209] Adenosine triphosphate 5mmol / L
[0210] Glutamic acid 5mmol / L
[0211] Carbamyl phosphate 5mmol / L
[0212] Monovalent phosphate 5mmol / L
[0213] Hydrogen cyanide 5mmol / L;
[0214] Reagent 2 with the following composition:
[0215] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
[0216] Sodium chloride 2mol / L
[0217] Carbamyl phosphate synthase 28000U / L
[0218] Hydrogen cyanide synthase 50000U / L;
[0219] Reagent 3 with the following composition:
[0220] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L
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