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Divalent combined oil-emulsion inactivated vaccine for preventing goat contagious pleuropneumonia and preparation method

A technology of pleuropneumonia and inactivated vaccines, applied in the direction of bacterial antigen components, antibacterial drugs, etc., can solve the problems of failure to obtain effective prevention and control, unsatisfactory use of preventive vaccines, and restrictions on the development of goat industry, and achieve the distribution of immune antigens Uniformity, high use safety and stability, good immune specificity and sensitivity

Inactive Publication Date: 2012-07-18
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the unsatisfactory effect of the preventive vaccine against goat infectious pleuropneumonia currently used in China, the disease has not been effectively prevented and controlled so far. Restricted the development of goat industry

Method used

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  • Divalent combined oil-emulsion inactivated vaccine for preventing goat contagious pleuropneumonia and preparation method
  • Divalent combined oil-emulsion inactivated vaccine for preventing goat contagious pleuropneumonia and preparation method
  • Divalent combined oil-emulsion inactivated vaccine for preventing goat contagious pleuropneumonia and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 : Take goat infectious pleuropneumonia main causative agent Mycoplasma ovis pneumonia "Y 98 " strain and Mycoplasma goat subspecies Guizhou local strain "Guiluo P G3 "The strain is a specific immune antigen, which is synthesized by inactivation and emulsification; each milliliter of "Y 98 "," Guiluo P G3 "The bacterial content is 10×10 6 ;

[0061] Its preparation steps are:

[0062] (1) Preparation of inactivated vaccine

[0063] ① Strain rejuvenation: take "Guiluo P G3 ", "Y 98 "The freeze-dried virus strain was injected into the trachea of ​​healthy goats, and observed for rejuvenation. After 7-10 days of onset, the lung, lymph, pleural effusion and other diseased tissues of the inoculated goat were cut to make a mother solution containing bacteria;

[0064] ② Inoculate healthy goats with the mother liquid twice, and after 7-10 days of onset, take lung, lymph, pleural effusion and other diseased tissues and inoculate them in the selective mycoplasm...

Embodiment 2

[0077] The main causative agent of goat infectious pleuropneumonia was Mycoplasma ovis pneumoniae "Y 98 " strain and Mycoplasma goat subspecies Guizhou local strain "Guiluo P G3 "The strain is a specific immune antigen, which is synthesized by inactivation and emulsification; each milliliter of "Y 98 "," Guiluo P G3 "The bacterial content is 10×10 9 ;

[0078] Its preparation steps are:

[0079] (1) Preparation of inactivated vaccine

[0080] ① Strain rejuvenation: take "Guiluo P G3 ", "Y 98 "The freeze-dried virus strain was injected into the trachea of ​​healthy goats, and observed for rejuvenation. After 7-10 days of onset, the lung, lymph, pleural effusion and other diseased tissues of the inoculated goat were cut to make a mother solution containing bacteria;

[0081] ② Inoculate healthy goats with the mother liquid twice, and after 7-10 days of onset, take lung, lymph, pleural effusion and other diseased tissues and inoculate them in the selective mycoplasma mediu...

Embodiment 3

[0093] Example 3 : Take goat infectious pleuropneumonia main causative agent Mycoplasma ovis pneumonia "Y 98 " strain and Mycoplasma goat subspecies Guizhou local strain "Guiluo P G3 "The strain is a specific immune antigen, which is synthesized by inactivation and emulsification; each milliliter of "Y 98 "," Guiluo P G3 "The bacterial content is 10×10 10 ;

[0094] Its preparation steps are:

[0095] (1) Preparation of inactivated vaccine

[0096]① Strain rejuvenation: take "Guiluo P G3 ", "Y 98 "The freeze-dried virus strain was injected into the trachea of ​​healthy goats, and rejuvenation was observed. After 7-10 days of onset, the lungs, lymph, pleural effusion and other diseased tissues of the inoculated goats were dissected to make a mother solution containing bacteria;

[0097] ② Inoculate healthy goats with the mother liquid twice, and after 7-10 days of onset, take lung, lymph, pleural effusion and other diseased tissues and inoculate them in the selective m...

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Abstract

The invention discloses a divalent combined oil-emulsion inactivated vaccine for preventing goat contagious pleuropneumonia and a preparation method. Sheep pneumonia mycoplasma 'Y98' strains and filiform mycoplasma goat subspecies Guizhou local plant 'Guiluo PG3' strains are taken as specific immunity antigens, and then a series of experimental studies, such as bacterial strain rejuvenation, bacterial strain amplifying culturing, inactivation, biopsy, emulsification, safety inspection, effect check, and application prevention, are performed, thereby synthesizing the divalent combined oil-emulsion inactivated vaccine. When the content of Y98 is more than or equal to 10*10<9> and the content of Guiluo PG3 is more than or equal to 10*10<9> in per millimeter antigen, the immune antibody positive rate is 100%, and the effective rate of anti-poison protection experiment is above 87.5%; the immune antibody positive rate in an application test reaches 90.7-96.3%; the test proves that the divalent combined oil-emulsion inactivated vaccine has excellent immunologic specificity and sensibility to the goat contagious pleuropneumonia, and the usage is reliable, safe and stable.

Description

technical field [0001] The invention relates to a veterinary oil emulsion inactivated vaccine and a preparation method thereof, in particular to a bivalent dual oil emulsion inactivated vaccine for preventing goat infectious pleuropneumonia and a preparation method thereof, belonging to the technical field of veterinary biological control. Background technique [0002] Goat infectious pleuropneumonia is a serious conditional infectious disease in the current goat breeding production in my country, and its pathogen is mainly Mycoplasma ovis pneumoniae "Y 98 " and Mycoplasma goat subspecies "P G3 ". Domestic experimental research shows that "Y 98 "Mainly popular in my country's Northwest, Northeast and other regions," P G3 "It is mainly prevalent in Southwest, South China and other regions. Due to the significant geographical differences in the epidemic characteristics of the disease in China, there is no unified vaccine product in China. In recent years, with the growing ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/116A61K39/02A61P31/04
Inventor 文正常潘淑惠尚以顺杨粤黔
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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