Stem cell cryopreserving box and cryopreservation method

A cryopreservation and stem cell technology, which is applied in the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of long time to open the refrigerator door, poor position markability, cumbersome operation, etc., and achieve shortened door opening time and convenient operation. Easy to use and operate

Inactive Publication Date: 2012-07-25
THE SECOND AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] (1) Stem cells of multiple patients are often stored in a deep-low temperature refrigerator, and the stem cells of different patients can only be stacked and stacked in disorder, and the position can be marked poorly, making the inside of the refrigerator appear chaotic;
[0004] (2) Different storage boxes are often in a freeze-thaw bonded state under low temperature environment. When taking out the storage box located in the lower layer, an external force is required to separate the two bonded storage boxes. The operation is cumbersome, and this operation also makes the If the refrigerator door is opened for too long, the temperature in the refri

Method used

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  • Stem cell cryopreserving box and cryopreservation method
  • Stem cell cryopreserving box and cryopreservation method
  • Stem cell cryopreserving box and cryopreservation method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example one:

[0032] figure 1 This is a schematic cross-sectional structure diagram of a stem cell cryopreservation box provided in an embodiment of this application.

[0033] Such as figure 1 As shown, 1 in the figure is a collection bag. The dry cell cryopreservation box includes: a box body 2, a box cover 3 and a plurality of partitions 4, wherein: a plurality of collection bags 1 and a plurality of partitions 4 are placed in the box 2 , The box cover 3 is located on the top of the box body 2 and is connected with the box body 2. The box cover 3 presses a plurality of partitions 4 and a plurality of collection bags 1 into the box body 2.

[0034] The box body 2 has a box bottom and four side walls, the top of which is an open end, that is, the top is provided with an opening. In order to adapt to the low-temperature environment during the freezing of stem cells, the material of the box 2 is a low-temperature resistant material. In the embodiment of the present application...

Example Embodiment

[0043] Embodiment two:

[0044] in figure 1 with figure 2 In this case, the box cover 3 cooperates with the slot on the box body 2 through the buckle 6 to press the collection bag 1 placed in the box body 2.

[0045] In addition, in other embodiments of this application, such as image 3 As shown, the pressure regulating and fixing device can also be a clamp column.

[0046] Such as image 3 As shown, 10 in the figure is a clamping column, and the clamping buckle 6 can be fixed to the clamping column 10, so by changing the clamping buckle 6 to connect with the clamping column 10 of different heights, the pressure of the cover 3 on the collection bag 1 can also be adjusted .

Example Embodiment

[0047] Embodiment three:

[0048] Figure 4 A method for cryopreservation of stem cells provided in the embodiments of this application.

[0049] Such as Figure 4 As shown, the method includes the following steps:

[0050] S100: Place multiple collection bags and multiple partitions alternately in a box with a bottom and side walls, and the partition at the top is the partition.

[0051] When placing collection bags and partitions, in order to facilitate pressure on the topmost partition, the stack height of multiple collection bags and multiple partitions is greater than the depth of the box, so that when the collection bags and partitions are placed, The top partition will emerge from the outside of the box to facilitate pressure on the top partition.

[0052] S200: Use the pressure adjusting fixing device and the buckle to apply pressure to the topmost partition in a direction pointing to the box body to flatten the multiple collection bags.

[0053] Since the collection bag is fi...

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PUM

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Abstract

The invention discloses a stem cell cryopreserving box and a cryopreservation method. The stem cell cryopreserving box comprises a box body, a box cover and baffle plates, wherein an opening is arranged at the top part of the box body, and pressure adjusting and fixing devices are arranged outside two opposite side walls on the box body; a plurality of collection bags and a plurality of baffle plates are arranged in the box body alternately, and the baffle plates are located at the topmost part of the alternate arrangement of the collection bags and the baffle plates; and the box cover is located at the top part of the box body and is connected with the pressure adjusting and fixing devices on the side walls of the box body by fasteners. Compared with the prior art, when the stem cell cryopreserving box and the cryopreservation method are used, stem cell samples of the same patient can be placed in one box body, and the box cover can press the collection bags into a flat shape, so that stem cells can be uniformly cooled; each box body, as an independent storage unit, is convenient to take and use; and when the box body is taken and used, the operation is simple, and the consumed time is shorter, so that the refrigerator door opening time is shortened, and the temperatures of other box bodies are slightly influenced.

Description

technical field [0001] The present application relates to the technical field of medical equipment, in particular to a stem cell cryopreservation box and a cryopreservation method. Background technique [0002] Stem cells are usually stored in a low-temperature refrigerator / liquid nitrogen tank at -85°C or -196°C according to the required storage period. For stem cells that have a short period from collection to use (<6 months), usually use a deep-low temperature refrigerator to store them at -85°C. At present, there is no unified operating standard for the cryopreservation of this type of stem cells, and the storage boxes selected by each unit are not the same. Since the general hard plastic (referring to the storage box body) is easy to freeze and crack at deep low temperature, most of them Simply use some box-shaped paper materials to temporarily hold the stem cells collected in the blood transfusion bag. Above-mentioned preservation method has following shortcoming ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 张曦刘耀陈幸华高蕾
Owner THE SECOND AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
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