Full-solid urase electrode and preparation method thereof

An all-solid-state, urease technology, applied in the field of electroanalytical chemical detection, can solve the problems of long time, high cost, need to add reagents, etc., and achieve the effects of fast response, simple production and low production cost.

Inactive Publication Date: 2014-06-11
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, spectrophotometry is mostly used in the determination of urea, which takes a long time and needs to add reagents, and is not suitable for the determination of urea in colored samples and biological fluids. Although the enzymatic method is more specific and accurate than the colorimetric method, it needs to be added every time. Enzyme, the cost is high, therefore, a simple, fast and cheap method is needed to replace the current clinical method

Method used

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  • Full-solid urase electrode and preparation method thereof
  • Full-solid urase electrode and preparation method thereof
  • Full-solid urase electrode and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0040] The preparation process and steps in this embodiment are as follows:

[0041] (1) Print a layer of silver paste on the polyvinyl chloride substrate by screen printing, and the silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10 -1 and the second reaction zone 10-2;

[0042] (2) Preparation of the reference electrode: the bovine serum albumin is formulated into an aqueous solution with a mass concentration of 5%; the nanometer MnO 2 Dispersed in carbon paste to form a mixture, nano MnO 2The mass concentration in carbon paste is 30%; Add the bovine serum albumin solution of mixture weight 12% in the mixture, be mixed with the second response layer slurry, print this response layer slurry with the method for screen printing, in the The second reaction area 10-2 prints the second response layer 4-2 so that it completely covers the second reaction area 10-2 and becomes ...

Embodiment 2

[0050] The preparation process and steps in this embodiment are as follows:

[0051] (1) Print a layer of silver paste on the polyvinyl chloride substrate by screen printing, and the silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10 -1 and the second reaction zone 10-2;

[0052] (2) Preparation of the reference electrode: the bovine serum albumin is prepared into an aqueous solution with a mass concentration of 5%; the nano-SnO 2 Dispersed in carbon paste to form a mixture, nano SnO 2 The mass concentration in carbon paste is 20%; Add the bovine serum albumin solution of mixture weight 7% in the mixture, prepare the second response layer slurry, print this response layer slurry with the method for screen printing, in the The second reaction area 10-2 prints the second response layer 4-2 so that it completely covers the second reaction area 10-2 and becomes a reference e...

Embodiment 3

[0060] The preparation process and steps in this embodiment are as follows:

[0061] (1) Print a layer of silver paste on the polyvinyl chloride substrate by screen printing, and the silver paste layer is divided into the first contact electrode 2-1, the second contact electrode 2-2, the conductive layer 9, and the first reaction zone 10 -1 and the second reaction zone 10-2;

[0062] (2) Preparation of reference electrode: bovine serum albumin is prepared into an aqueous solution with a mass concentration of 5%; 2 Dispersed in carbon paste to form a mixture, nano RuO 2 The mass concentration in carbon paste is 10%; Add the bovine serum albumin solution of mixture weight 2% in the mixture, prepare the second response layer slurry, print this response layer slurry with the method for screen printing, in the The second reaction area 10-2 prints the second response layer 4-2 so that it completely covers the second reaction area 10-2 and becomes a reference electrode, and the sec...

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Abstract

The invention discloses a full-solid urase electrode and a preparation method thereof and belongs to the technical field of electroanalytical chemistry detection. The urase electrode is prepared through the following steps: printing metal oxide and urase onto a substrate by utilizing a screen printing technology; and forming a layer of uniform complex film on the surface of an electrode. The urase electrode prepared by the invention can be directly applied to the rapid detection on the concetration of urea, is good in catalytic performance and is high in stability. The urase electrode is small and portable, rapid in response, simple in manufacturing process, low in manufacturing cost, suitable for volume production and especially suitable for real-time detection such as field rescue, clinical examination and environmental monitoring.

Description

technical field [0001] The invention belongs to the technical field of electroanalytical chemical detection, and in particular relates to an all-solid-state urease electrode and a preparation method thereof. Background technique [0002] In recent years, due to the development of chemical sensors, especially the research on electrochemical sensors, and their application expansion in the fields of biomedicine and the environment, a simple electrode production technology is needed, which has the same chemical properties in mass production, reproducibility, and Sensitive, inexpensive and disposable sensors. The process of screen printing is not only reproducible, but also provides a broad imagination space for people to design suitable electrode shapes. The size and shape of the sensor electrodes produced can be changed, suitable for mass production. Good stability and consistency Electrochemical sensor, easy miniaturization and integration. Screen printing equipment is simpl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327
Inventor 陈国松金青黄颖张之翼唐美华李成东
Owner NANJING TECH UNIV
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