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Method for extracting mogroside V and mannitol from corsvenor momordica fruit

A technology of mogroside and mannitol, which is applied in the field of extracting mogroside V and mannitol at the same time, extracting mogroside V and mannitol, can solve the problem that mannitol is not well utilized, and save resources and improve utilization rate , the effect of reducing production costs

Inactive Publication Date: 2012-09-19
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0039] To sum up, at present, the utilization of Luo Han Guo is mainly used to extract mogroside V, which is used as a sweetener, while mannitol, which is used for antitussive effect, has not been well utilized

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Use 100 kg of dried Luo Han Guo to break the shell and crush, mix 2000L of 50% ethanol, heat to 70°C for reflux extraction for about 4 hours; The solution was extracted with 300L ethyl acetate, and the solution containing ethyl acetate was concentrated under reduced pressure at 70°C until 2.35kg of dry matter was obtained; the dry matter was dissolved in 25L of water, and put on an AB-8 macroporous adsorption resin column , use ethanol concentration of 20-60%, gradient elution, collect 160L of eluent I with 60% ethanol; after concentrating eluent I to 25L, add 25L of acetone solution to a final concentration of about 50%, crystallize overnight at room temperature and Recrystallize to obtain 1.12 kg of pure mogroside V; wash the 7320 type strong acidic cation exchange resin on the ethyl acetate-free solution with water with a pH value of 4 for three times the column volume, and then use hydrochloric acid to adjust the pH value of the eluent to Adjust it at about 6.2-6.8;...

Embodiment 2

[0063] Use 200 kg of dried Luo Han Guo to break the shell and crush, mix 4500L of 50% ethanol, heat to 70°C for reflux extraction for about 4 hours; filter and remove residue, concentrate under reduced pressure at 70°C to 500L without alcohol smell; The solution was extracted with 500L ethyl acetate, and the solution containing ethyl acetate was concentrated under reduced pressure at 70°C until 4.86kg of dry matter was obtained; the dry matter was dissolved in 40L of water, and put on an AB-8 macroporous adsorption resin column , use ethanol concentration of 20-60%, gradient elution, collect 250L of eluent I with 60% ethanol; after concentrating eluent I to 30L, add 30L of acetone solution to a final concentration of about 50%, crystallize overnight at room temperature and Recrystallize to obtain 2.36 kg of pure mogroside V; wash the 7320 type strong acidic cation exchange resin with water with a pH value of 4 on the solution without ethyl acetate, and wash the eluent with hydr...

Embodiment 3

[0065] Use 400 kg of dried Luo Han Guo to break the shell and crush, mix 10000L of 50% ethanol, heat to 70°C for reflux extraction for about 4 hours; filter and remove residue, concentrate under reduced pressure at 70°C to 900L without ethanol smell; The solution was extracted with 900L ethyl acetate, and the solution containing ethyl acetate was concentrated under reduced pressure at 70°C until 10.38kg of dry matter was obtained; the dry matter was dissolved in 60L of water, and put on an AB-8 macroporous adsorption resin column , use ethanol concentration of 20-60%, gradient elution, collect 400L of eluent I with 60% ethanol; after concentrating eluent I to 50L, add 50L of acetone solution to a final concentration of about 50%, crystallize overnight at room temperature and Recrystallize to obtain 4.85 kg of pure mogroside V; wash the 7320 type strong acidic cation exchange resin with water with a pH value of 4 on the solution without ethyl acetate, and wash the eluent with hy...

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Abstract

Disclosed is a method for extracting mogroside V and mannitol from corsvenor momordica fruit. Dried siraitia grosvenori is subjected to shell breaking, water extraction, filtering, extraction, resin absorption and elution in ethanol with different concentration, different eluents are subjected to decoloration, and crystallization and recrystallization are adopted for obtaining the pure mogroside V and the pure mannitol respectively. According to the method, utilization rate of the corsvenor momordica fruit is greatly improved, the corsvenor momordica fruit resource is saved, the operation is simple, production costs of the mogroside V and the mannitol are greatly reduced, the production process is simplified, all reagents are non-toxic, cheap and mass-produced chemical reagents, mature routine techniques for reagent recovery can be used during the whole process, and therefore, the discharge of waste to the environment is greatly reduced.

Description

technical field [0001] The invention belongs to the field of natural product processing, and relates to a method for extracting mogroside V and mannitol, and more specifically relates to a method for simultaneously extracting mogroside V and mannitol from the fruit of Luo Han Guo. Background technique [0002] Luo Han Guo (Corsvenor Momordica Fruit), a climbing herb; root perennial, hypertrophic, spindle-shaped or nearly spherical; stems and branches slightly thick, with grooves, first batch of yellowish-brown pilose and black verrucous glandular scales, the back hairs gradually fall off and become closer hairless. The petiole is 3-10 cm long, covered with the same hairy indumentum and glandular scales as the branches; the leaves are membranous, ovate-cordate, triangular-ovate or broadly ovate-cordate, 12-23 cm long, 5-17 cm long, Apex acuminate or long acuminate, base heart-shaped, cove semicircular or nearly round, 2-3 cm deep, 3-4 cm wide, wavy-shaped margin, due to smal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00C07C31/26C07C29/76
Inventor 饶力群
Owner HUNAN AGRICULTURAL UNIV
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