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Pretreatment method for improving enzyme hydrolysis rate of lignocellulose

A technology for lignocellulose and enzymatic hydrolysis, applied in the direction of fermentation, can solve the problem of unsatisfactory cellulase hydrolysis rate, and achieve the effect of clean and efficient separation

Active Publication Date: 2015-03-11
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology

Although the existing enzymatic hydrolysis methods are relatively mature, due to the influence of lignin in lignocellulose, the enzymatic hydrolysis rate of cellulose has not been ideal

Method used

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  • Pretreatment method for improving enzyme hydrolysis rate of lignocellulose
  • Pretreatment method for improving enzyme hydrolysis rate of lignocellulose
  • Pretreatment method for improving enzyme hydrolysis rate of lignocellulose

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Embodiment Construction

[0029] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but not as a limitation of the present invention.

[0030] The wood powder raw material in this example is fast-growing triploid Populus tomentosa, three-year-old, collected from Shandong Province. After the raw material is crushed, pass through a 0.8mm sieve, extract with phenyl alcohol Soxhlet in a volume ratio (2:1) for 6 hours, and take a dewaxed 80-100 mesh dry sample for use. Ionic liquid [C 2 mim]OAc was purchased from Lanzhou Institute of Chemical Physics with a purity of ≥98.5%. Cellulase (Celluclast 1.5L) and β-glucosidase were purchased from Sigma Aldrich. All other reagents used were of analytical grade or reagent grade and were directly purchased and used without further purification.

[0031] figure 1 It is a schematic flow chart of the lignocellulose pretreatment method for improving the enzymatic hydrolysis rate of t...

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Abstract

The invention discloses a pretreatment method for improving the enzyme hydrolysis rate of lignocellulose. The method comprises the following steps of: a, dissolving dewaxed wood flour into an ionic liquid to obtain an ionic liquid solution; b, transferring the ionic liquid solution into a reversed phase solvent to perform regeneration; c, filtering the ionic liquid solution which is subjected to the regeneration in the reversed phased solvent, and performing freeze-drying to obtain regenerated wood flour; d, performing mild alkali treatment on the regenerated wood flour which is obtained in the step c for removing lignin components and hemi-cellulose components to obtain residues after the alkali treatment; and e, performing enzyme hydrolysis on the residues after the alkali treatment to obtain glucose. By using the method, the enzyme hydrolysis conversion rate of cellulose in the lignocellulose is improved.

Description

technical field [0001] The invention relates to the technical field of lignocellulose treatment, in particular to a lignocellulose pretreatment method for improving enzymatic hydrolysis rate. Background technique [0002] With the decrease of oil resources, the continuous rise of oil prices and people's widespread concern about environmental issues, lignocellulosic resources have become an ideal substitute for petrochemical resources. The main components of lignocellulosic raw materials are cellulose (35%-50%), hemicellulose (20%-40%) and lignin (15%-25%). In the lignocellulosic raw material, these three main components constitute the supporting skeleton of the plant body, in which the cellulose constitutes microfibers and constitutes the network skeleton of the fiber cell wall, while hemicellulose and lignin are covalently bonded and filled in the fiber Between the microfibers, a "concrete structure" similar to the interlacing of reinforced concrete is formed. For the nee...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C08B37/14C08H7/00
Inventor 袁同琦许凤孙润仓张利鸣孙少妮
Owner BEIJING FORESTRY UNIVERSITY
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