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Method for measuring gibberellin in fruits by aid of molecularly imprinted electrochemically modified electrode

A technology of molecular imprinting and modified electrodes, which is applied to the electrochemical variables of materials, measuring devices, scientific instruments, etc., can solve the problems of cumbersome operation and a large amount of organic solvents, and achieve the effects of high sensitivity, long service life, and wide linear range of determination

Inactive Publication Date: 2012-10-03
CHANGZHOU HOUXIAO YUHENG CURTAIN WALL
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

These methods are cumbersome to operate and require the use of large amounts of organic solvents

Method used

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  • Method for measuring gibberellin in fruits by aid of molecularly imprinted electrochemically modified electrode
  • Method for measuring gibberellin in fruits by aid of molecularly imprinted electrochemically modified electrode

Examples

Experimental program
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Embodiment

[0017] (1) Dopamine-labeled gibberellin:

[0018] Take 5.0×10 -4 mol / L gibberellin standard solution 0.5mL in a stoppered colorimetric tube, add 0.01mol / L N-hydroxysuccinimide (NHS) 2mL, shake well, let stand for 10min, then add 2.5×10 -3 mol / L dopamine (DA) 2mL, shake well and add 0.01mol / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) 2mL as dehydration condensation agent Make gibberellin (GA) and dopamine (DA) carry out dehydration reaction; After reacting for 20min, the concentration obtained is 2.5×10 -5 mol / L dopamine-labeled gibberellin (DA-GA) standard solution;

[0019] (2) Preparation of molecularly imprinted modified electrodes:

[0020] Place the cleaned working electrode in a molar concentration of 5.0×10 -4 mol / L methylene blue monomer, the molar concentration is 2.5×10 -4 mol / L gibberellin and borax buffer solution with a molar concentration of 0.2mol / L and pH=9.2 were used for electrochemical polymerization, with 0.2mol / L KCl as the sup...

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Abstract

The invention discloses a method for measuring gibberellin in fruits by the aid of a molecularly imprinted electrochemically modified electrode. The gibberellin and methylene blue are mixed and are electrochemically polymerized to form a membrane on the surface of a glassy carbon electrode, and a molecularly imprinted electrochemical sensor is prepared after the gibberellin is eluted. Along with the increase of the quantity of the gibberellin which is marked by dopamine on various recognition sites on the molecular imprinted membrane and is eluted, current of oxidation peaks is greatly reduced, and accordingly the electrochemical analysis method for measuring the trace gibberellin is built. Good linear relation between peak current I and the gibberellin within the concentration range from 2.5X10-8 to 1.05X10-6mol / L is good. The method overcomes various shortcomings such as excessive complexity in the prior art, is high in measuring sensitivity, wide in measuring linear range and low in detection limit, and has an excellent effect in detecting the gibberellin with the concentration of 10-8mol / L in the fruits.

Description

technical field [0001] The invention relates to a method for measuring gibberellin in fruit by using a molecularly imprinted electrochemically modified electrode. Background technique [0002] Molecularly imprinted electrochemical sensors combine the advantages of molecularly imprinted polymers and electrochemical sensors, which not only exhibit high specificity, but also have low cost, easy miniaturization, automation, high detection limit and long service life. And the preparation process is simple and so on, so it is widely used. Gibberellin is a large class of plant hormones. As a macrolide antibiotic, the United States, Japan and other countries stipulate that the maximum residue limit of gibberellin (GA) in fruits and vegetables is 0.2 μg / g. The determination of gibberellin has attracted much attention. At present, the determination of gibberellin mainly adopts high performance liquid chromatography, square wave voltammetry, fluorescence photometry, Folin reagent pho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327
Inventor 李建平常川魏小平
Owner CHANGZHOU HOUXIAO YUHENG CURTAIN WALL
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