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Preparing method of anthrax vaccines

A vaccine and anthrax technology, applied in the direction of bacterial antigen components, antibacterial drugs, etc., can solve the problems of low viable bacteria count and low viable bacteria rate, and achieve the effect of reducing production cost, improving product qualification rate and saving manpower

Active Publication Date: 2014-01-08
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The inventors of the present invention creatively proposed a new preparation method of live anthrax vaccine for humans with scratches on the skin through a large number of experimental studies, which solved the technical problems of the existing preparation method with low number of live bacteria and low live bacteria rate

Method used

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  • Preparing method of anthrax vaccines
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  • Preparing method of anthrax vaccines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Comparison of using 50% glycerol solution and water for injection to harvest cultures to prepare live anthrax vaccines (shaker shaker and mix well)

[0028] 1. Take 2 tubes of CMCC63001 (A16R) anthrax strains to inoculate thick Ginger agar medium, and incubate at 33-34°C for 18-24 hours.

[0029] 2. Rinse the bacterial lawn with Thick Ginger Broth, inoculate in large tubes of Thick Ginger Broth, inoculate 2m L of bacterial solution in each tube, and incubate at 33-34°C for 18-24 hours.

[0030] 3. Collect the seed culture in the large tube of thick Ginger broth, inoculate it into thick Ginger agar Kjeldahl flasks, inoculate 40 of them in total, and inoculate 2m L of bacterial solution in each Kjeffer flask, 33-34 Cultivate at ℃.

[0031] 4. Harvesting and preparing finished products

[0032] On the fifth day of thick Ginger agar Kirschner bottle culture, a bottle was randomly selected, and the bacterial lawn smear was taken for inspection. The mature typical...

Embodiment 2

[0085] Example 2 Comparison of using 50% glycerol solution and water for injection to harvest cultures to prepare live anthrax vaccines respectively (shaking and mixing by hand)

[0086] 1. Take two CMCC63001 (A16R) anthrax strains and inoculate thick Ginger agar medium, and incubate at 33-34°C for 18-20 hours.

[0087] 2. Rinse the bacterial lawn with Thick Ginger Broth, inoculate in large tubes of Thick Ginger Broth, inoculate 2m L of bacterial solution in each tube, and incubate at 33-34°C for 18-24 hours.

[0088] 3. Collect the seed culture in the large tube of thick Ginger broth, inoculate it into thick Ginger agar Kjeldahl flasks, inoculate 30 of them in total, and inoculate 2m L of bacterial solution in each Kjeffer flask, 33-34 Cultivate at ℃.

[0089] 4. Harvesting and preparing finished products

[0090] 4.1 Harvesting: On the fifth day of thick Ginger agar Kirschner bottle culture, randomly select a bottle, take a smear of the bacterial lawn for inspection, the m...

Embodiment 3

[0107] Example 3 Preparation of Live Anthrax Vaccine by Harvesting Cultures Using Water for Injection (Pipe and mix with a pipette and shake by hand)

[0108] 1. Take 2 tubes of CMCC63001 (A16R) anthrax strains and inoculate beef digestate agar medium, and incubate at 33-34°C for 18-24 hours.

[0109] 2. Rinse the bacterial lawn with beef digestive juice, inoculate in large tubes of beef digestive juice, inoculate 2m L bacterial solution in each tube, and incubate at 33-34°C for 18-24 hours.

[0110] 3. Collect the seed culture in the large tube of beef digestion solution, inoculate it into beef digestion solution agar Kirschner flasks, inoculate 30 of them in total, inoculate 2m L of bacterial liquid in each Kirschner flask, and incubate at 33-34°C.

[0111] 4. Harvesting and preparing finished products

[0112]4.1 Harvesting: On the fifth day of culturing beef digestate agar Kirschner flasks, randomly select a bottle and take a smear of the bacterial lawn for inspection. Th...

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Abstract

The invention discloses a preparing method of live anthrax vaccines for people with percutaneous scarification, comprising the steps of: scraping a culture into water for injection when harvesting; blowing and / or shaking by using a suction tube to form a uniform suspension; adding equivoluminal sterile glycerol to the suspension; and uniformly mixing to obtain a stock solution.

Description

technical field [0001] The invention relates to a preparation method of anthrax vaccine, in particular to a preparation method of live anthrax vaccine for human with skin scratches. Background technique [0002] Anthrax is a zoonotic acute infectious disease caused by Bacillus anthracis. It mainly exists in herbivores (sheep, cattle, horses, mules, donkeys, camels, etc.) and livestock communities, and causes extensive environmental pollution. Due to the strong resistance of Bacillus anthracis spores to the outside world, the pollution can continue to exist. Most people are infected by contact with sick animals or slaughtering and eating anthrax sick animals. Anthrax is prevalent all over the world, mainly in developing countries. Some countries have used Bacillus anthracis as a biological weapon. After 9 / 11, terrorists also used anthrax bacteria to carry out terrorist operations, which brought great panic to people. [0003] The human anthrax vaccine used in my country ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/07A61P31/04
Inventor 王国治魏东
Owner NAT INST FOR FOOD & DRUG CONTROL
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