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Microarray comprising immobilisation particles

A microarray and particle technology, applied in the field of microarrays, can solve the problem that the capture molecules cannot be directly combined and washed away.

Inactive Publication Date: 2012-11-21
ROBERT BOSCH GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, capture molecules cannot directly bind to untreated glass or plastic surfaces and are washed away in subsequent steps

Method used

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  • Microarray comprising immobilisation particles
  • Microarray comprising immobilisation particles
  • Microarray comprising immobilisation particles

Examples

Experimental program
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Embodiment Construction

[0052] Figure 1a It is shown that the microarray comprises a carrier substrate 1 and a large number of immobilized particles 2 for immobilizing the capture molecules, wherein each of the immobilized particles 2 has a first subregion connected to the carrier substrate 1 Section 2a and an open second partial section 2b. Figure 1a It is shown that the open second subsection 2 b is accessible here from outside the carrier substrate 1 . Figure 1a It is stated here that in this way the immobilization particles 2 provide local potential binding regions for the capture molecules to be immobilized.

[0053] also, Figure 1a It is shown that the first subsection 2 a is accordingly embedded or pressed into the carrier substrate 1 in a form-fitting manner, wherein the second subsection 2 b protrudes from the carrier substrate 1 . come out. as in Figure 1a As shown in , the immobilized particles 2 are embedded in the carrier substrate 1 such that a spherical hemisphere of the immobiliz...

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Abstract

The invention relates to a microarray which comprises a carrier substrate (1). In order, inter alia, to increase the sensitivity, the microarray comprises a plurality of immobilisation particles (2) for immobilising capture molecules. Each immobilisation particle comprises a first sub-section (2a) bonded to the carrier substrate (1) and a second sub-section (2b) which is exposed.

Description

technical field [0001] The present invention relates to a microarray, a device for producing such a microarray, a method for producing such a microarray and uses thereof. Background technique [0002] Microarrays are tools of biological analysis for the identification and quantification of individual molecular groups from complex mixtures by special key-lock-binding at precisely defined spots (Spots) on a carrier substrate. Microarrays are used for the identification and quantification of nucleic acids, proteins, cells and smaller molecules. [0003] Microarrays are produced conventionally by applying (spotting) capture molecules dissolved in a liquid onto a carrier substrate and subsequently immobilizing them, for example with drying of the liquid. The capture molecules can here be formed piecewise on the carrier substrate (synthesis on a chip) or printed onto the carrier substrate after synthesis. In this case, the capture molecules are attached to the surface of the car...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J19/00
CPCB01J2219/00468B01J2219/00387B01J2219/00382B01J2219/00585B01J2219/00648B01J2219/005B01J19/0046B01J2219/00533B01J2219/00466B01J2219/00596
Inventor M.施图姆贝尔M.道布J.鲁普
Owner ROBERT BOSCH GMBH