Method for treating heavy metal-containing pollutant wastewater
A treatment method and technology for heavy metal ions, which are applied in biological water/sewage treatment, water/sludge/sewage treatment, water pollutants, etc., can solve the problems of high cost and complicated operation, and achieve low cost, simple operation, and reduced The effect of heavy metal content
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Embodiment 1
[0079] Embodiment 1: the treatment that contains heavy metal ion polluted waste water
[0080] The processing steps of this embodiment are as follows:
[0081] Step A: Bacterial Culture
[0082] (i) Rhodobacter sphaeroides was purchased from China General Microorganism Culture Collection (No. 1.2182). Use an inoculation loop to pick up the Rhodobacter sphaeroides on the solid medium, place it on a petri dish containing 8g of solid medium for streaking, and then culture it at a temperature of 30°C and a pH of 7 for 48 hours;
[0083] The composition of the solid medium is as follows: 15g / L agar, 1.5g / L sodium acetate, 0.5g / L peptone, 0.6g / L sodium bicarbonate, 0.4g / L sodium thiosulfate, 0.3g / L chloride Sodium, 0.12g / L magnesium sulfate and 0.04g / L potassium dihydrogen phosphate; the solid medium was sterilized at a temperature of 121°C for 20 minutes, and cooled for use;
[0084] (ii) From the solid medium cultivated in (i), select a single colony with a diameter greater tha...
Embodiment 2
[0098] Embodiment 2: the treatment that contains heavy metal ion polluted waste water
[0099] This embodiment is carried out in the same manner as Example 1, except that the heavy metal used is Pb 2+ , Pb in wastewater 2+ The concentration is 0-150mg / L.
[0100] ICP-AES was used to determine the change of metal Pb concentration in water before and after adding Rhodobacter sphaeroides, Pb 2+ The removal rate results are attached figure 2 shown. It can be seen from the figure that the removal rate of Pb decreases with the increase of metal concentration. When the concentrations of Pb in the solution are 0, 20, 50, 100, and 150 mg / L, the removal rates of Pb are 93%, 91%, 48%, 40%, and 32% when reacting in the microbial treatment device for different times , to achieve a good removal effect.
Embodiment 3
[0101] Embodiment 3: the treatment that contains heavy metal ion polluted waste water
[0102] This embodiment is carried out in the same manner as Example 1, except that the heavy metal used is Zn 2+ , Zn in wastewater 2+ The concentration is 500mg / L.
[0103] Determination of metal Zn in water before and after adding Rhodobacter sphaeroides by ICP-AES 2+ Concentration change, Zn 2+ The removal rate was 80%, achieving a good removal effect.
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