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Method for increasing phytophthora capsici oospore output and promoting oospore germination
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A technology of Phytophthora capsicum, oospores, applied in the direction of using spores, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as different conditions
Active Publication Date: 2014-08-13
CHINA AGRI UNIV
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[0004] There are also relevant reports on the production and germination of Phytophthora oospores, but the conditions required for the production and germination of Phytophthora strains of the same genus and different species are different. Research on the production and germination conditions of oospores such as mold and Phytophthora phytophthora. There is no domestic report on the research on oospores of Phytophthora capsici
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Embodiment 1
[0031] Embodiment 1, improve oospore output
[0032] 1. Screening of medium
[0033] 10%V 8 Medium: V 8 Vegetable juice 100ml, CaCO 3 0.2g, 14g agar powder, add distilled water to make up to 1L, autoclave, 121°C, 20min, when cooled to 40~50°C, pour into sterilized petri dish for later use.
[0034] SLA medium: β-sitosterol 20mg, lecithin 500mg, CaCO 3 0.2g, aspartic acid 10mg, V 8 Vegetable juice 100ml, agar 2g and deionized water 900ml. Put the lecithin in 50ml water, homogenize it with a homogenizer (500r / min) for 3 minutes, fully break up the lecithin, add V 8 Juice, CaCO 3 , Agar, add water to 1000ml, dissolve β-sitosterol with a small amount (several milliliters) of dichloromethane and mix it with the medium. Dissolve aspartic acid in a small amount of water and pack it separately in the Erlenmeyer flask, sterilize at 121°C for 20 minutes, when the medium drops to about 50°C, add the aspartic acid solution into the medium, shake well and pour Store in a sterili...
Embodiment 2
[0049] Embodiment 2, the method for promoting Phytophthora capsici oospore germination
[0050] (1) Preparation of medium
[0051] S+L medium: 0.1g lecithin, 10ml basal culture solution [MgSO 4 ·7H 2 O 100mg, (NH 4 ) SO 4 100mg, K 2 HPO 4 60mg, CaCl 2 2H 2 O 30mg, KH 2 PO 4 30mg, ZnSO 4 ·7H 2 O 3mg, distilled water 100ml], glucose 0.04g, agar powder 14g, distilled water to 1L. Autoclaved, 121°C, 20min. After cooling to about 50°C, add the final concentration of 50 μg / ml rifampicin (rifampicin), 50 μg / ml ampicillin (penicillin), 50 μg / ml pentachloronitrobenzene (PCNB, pentachloronitrobenzene) and 50 μg / ml carbendazim (carbendazim) Shake well and pour into sterilized Petri dishes for later use;
[0052] (2) Treatment of Phytophthora capsici oospores at different post-ripening stages: hybrid combination Sx-3×Sx-22, cultured in 10% V 8 On the medium plate, place it under 25°C dark condition and cultivate it for 5-7d. After a large amount of oospores are observed ...
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Abstract
The invention provides a method for increasing phytophthora capsici oospore output and promoting oospore germination. The method comprises the following steps: culturing phytophthora capsici strains in a dark place at the temperature of 25-28 DEG C by 10% of V8 vegetable juice; keeping phytophthora capsici oospores in the dark place for 15-45 days; and processing the oospores by 0.05-0.25% by mass of potassium permanganate, preparing an oospore suspension solution, coating the oospore suspension solution onto an S+L culture medium, and inducing oospore germination by alternately illuminating for 16 h by a daylight lamp or 8 h by a black light lamp. According to the method, an economic, simple and effective method is provided for inducing a large amount of phytophthora capsici oospores to be generated and germinated in a short time, and a guarantee is provided for the application of a large amount of phytophthora capsici single oospore groups to drug resistance or other genetics researches.
Description
technical field [0001] The invention relates to a method for increasing the yield of oospores of Phytophthora capsici and promoting the germination of oospores. Background technique [0002] Pepper blight caused by Phytophthora capsici and blight on Solanaceae and Cucurbitaceae crops such as tomato and pumpkin are currently widespread in Asia, America, Europe and Australia, and also occur in most provinces and cities in my country. Pepper blight spreads rapidly and is highly destructive to host plants. It can occur from the seedling stage to the fruiting stage, seriously harming the stems, leaves, and fruits of the plant, and eventually leading to dead seedlings, rotten fruits, and even crop failure. Due to disease-resistant varieties and effective agricultural cultivation management measures, the damage has gradually increased and is difficult to control. It has become an important disease in vegetable production in my country. [0003] Phytophthora capsici belongs to heter...
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