Microbial environment protectant
An environmental protection agent and microorganism technology, applied in microorganisms, biocides, microorganism-based methods, etc., can solve the problems of microbial enzyme inactivation, affect the use effect, and cannot grow normally, and achieve strong degradation conversion ability, reduce content, reduce The effect of the generation of adult flies
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[0036] Preparation of Rhodopseudomonas palustris bacteria:
[0037] a. Make shake flask seed solution: inoculate a single colony of domesticated Rhodopseudomonas palustris into liquid medium, and cultivate at 30°C for 48 hours to obtain shake flask seed solution;
[0038] Described liquid culture medium is the mixed solution prepared in the following ratio: 0.2 gram of sodium acetate, 0.1 gram of sodium chloride, 0.1 gram of magnesium sulfate, 0.05 gram of potassium dihydrogen phosphate, 0.1 gram of ammonium sulfate, 0.01 gram of calcium chloride, 100ml of water, pH 6.6; sterilize at 121°C for 30 minutes, cool for later use;
[0039] b. Production of primary seed liquid: Inoculate the Rhodopseudomonas palustris shake flask seed liquid obtained in step a with an inoculation amount of 10% by volume into the primary seed liquid medium of Rhodopseudomonas palustris in an anaerobic environment at 30°C Cultivate for 72 hours, the light intensity is 1000lx, the number of bacteria...
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